(Circular) (Six-base) MAPSORT of: pFN21AB8941.seq Check: 1803 from: 1 to: 5608 pFN21AB8941 5608 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2381 2381 Size: 5608 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 2951 1937 Size: 927 87 4594 Fragments arranged by size: 4594 927 87 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2496 3671 5055 5323 1632 Size: 864 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1175 864 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2651 2683 2651 Size: 32 5576 AlwNI CAG_nnn'CTG Cuts at: 3351 4923 3351 Size: 1572 4036 ApaI G_GGCC'C Cuts at: 2359 2698 2359 Size: 339 5269 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2248 2344 2933 1870 Size: 73 305 96 589 4545 Fragments arranged by size: 4545 589 305 96 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2224 2929 4384 618 Size: 325 217 627 437 705 1455 1842 Fragments arranged by size: 1842 1455 705 627 437 325 217 BanII G_rGCy'C Cuts at: 1391 2359 2609 2698 2730 2927 1391 Size: 968 250 89 32 197 4072 Fragments arranged by size: 4072 968 250 197 89 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 1903 Size: 34 1862 34 3678 Fragments arranged by size: 3678 1862 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2229 2359 2698 3669 4825 1790 Size: 439 130 339 971 1156 2573 Fragments arranged by size: 2573 1156 971 439 339 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2607 3339 4297 Size: 416 53 83 399 24 1190 732 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1190 958 732 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2744 2865 4268 1239 Size: 1505 121 1403 2579 Fragments arranged by size: 2579 1505 1403 121 Bpu10I CC'TnA_GC Cuts at: 2112 2687 2112 Size: 575 5033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2790 2820 1178 Size: 30 165 30 1387 30 3966 Fragments arranged by size: 3966 1387 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 1186 Size: 1285 4323 BseYI C'CCAG_C Cuts at: 2060 2199 2234 2276 2358 2812 2891 4811 5307 Size: 139 35 42 82 454 79 1920 496 Cuts at: 5307 2060 Size: 2361 Fragments arranged by size: 2361 1920 496 454 139 82 79 42 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5035 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 3669 3754 4825 2730 Size: 939 85 1071 3513 Fragments arranged by size: 3513 1071 939 85 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 BsmBI CGTCTCn'nnnn_ Cuts at: 2316 2316 Size: 5608 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2359 2609 2698 2730 2927 3669 Size: 399 439 130 250 89 32 197 742 Cuts at: 3669 3754 4825 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 250 197 130 89 85 32 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2381 2963 844 Size: 1537 582 3489 Fragments arranged by size: 3489 1537 582 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2399 2464 4252 4439 1700 Size: 110 589 65 1788 187 2869 Fragments arranged by size: 2869 1788 589 187 110 65 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603 Size: 966 367 385 17 1452 86 12 346 Cuts at: 5603 1972 Size: 1977 Fragments arranged by size: 1977 1452 966 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2529 513 Size: 552 939 525 3592 Fragments arranged by size: 3592 939 552 525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 3124 3918 3938 3124 Size: 794 20 4794 Fragments arranged by size: 4794 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573 Size: 54 1425 50 275 18 1225 889 1629 Cuts at: 5573 8 Size: 43 Fragments arranged by size: 1629 1425 1225 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3538 1353 Size: 2185 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2728 2728 Size: 5608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2496 2744 2865 3671 4268 5055 5323 Size: 393 864 248 121 806 597 787 268 Cuts at: 5323 1239 Size: 1524 Fragments arranged by size: 1524 864 806 787 597 393 268 248 121 EcoNI CCTnn'n_nnAGG Cuts at: 2486 2486 Size: 5608 EcoO109I rG'GnC_Cy Cuts at: 2057 2355 2806 2057 Size: 298 451 4859 Fragments arranged by size: 4859 451 298 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2067 2076 2085 4755 1678 Size: 389 9 9 2670 2531 Fragments arranged by size: 2670 2531 389 9 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2790 2822 4249 Size: 32 135 32 896 32 290 32 1427 Cuts at: 4249 4281 4323 4355 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1427 896 290 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2122 2865 2952 3198 669 Size: 988 281 184 743 87 246 3079 Fragments arranged by size: 3079 988 743 281 246 184 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3566 3925 4084 518 Size: 648 2400 359 159 2042 Fragments arranged by size: 2400 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2647 2843 3024 3701 4849 5094 1606 Size: 1041 196 181 677 1148 245 2120 Fragments arranged by size: 2120 1148 1041 677 245 196 181 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4117 Fragments arranged by size: 4117 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2871 2966 4511 1132 Size: 97 1642 95 1545 2229 Fragments arranged by size: 2229 1642 1545 97 95 PasI CC'CwG_GG Cuts at: 1348 2218 1348 Size: 870 4738 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2806 2057 Size: 749 4859 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2355 2694 2355 Size: 339 5269 PspXI vC'TCGA_Gb Cuts at: 2344 2344 Size: 5608 PstI C_TGCA'G Cuts at: 830 2273 2960 830 Size: 1443 687 3478 Fragments arranged by size: 3478 1443 687 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2647 2843 2647 Size: 196 5412 RsrII CG'GwC_CG Cuts at: 2402 2402 Size: 5608 SacI G_AGCT'C Cuts at: 2730 2730 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2863 2950 1936 Size: 927 87 4594 Fragments arranged by size: 4594 927 87 SanDI GG'GwC_CC Cuts at: 2057 2806 2057 Size: 749 4859 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 2297 3856 5568 2297 Size: 1559 1712 2337 Fragments arranged by size: 2337 1712 1559 SfcI C'TryA_G Cuts at: 826 1045 2068 2269 2956 4091 4772 4963 826 Size: 219 1023 201 687 1135 681 191 1471 Fragments arranged by size: 1471 1135 1023 687 681 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2344 3712 4613 4875 820 Size: 197 926 401 1368 901 262 1553 Fragments arranged by size: 1553 1368 926 901 401 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 2601 1517 Size: 1084 4524 StyI C'CwwG_G Cuts at: 513 1065 2004 2832 3047 513 Size: 552 939 828 215 3074 Fragments arranged by size: 3074 939 828 552 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2587 2905 3635 3652 3805 3990 4435 Size: 1255 1007 318 730 17 153 185 445 Cuts at: 4435 325 Size: 1498 Fragments arranged by size: 1498 1255 1007 730 445 318 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 3854 5566 Size: 274 80 33 51 1443 318 1559 1712 Cuts at: 5566 96 Size: 138 Fragments arranged by size: 1712 1559 1443 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 2344 1943 Size: 401 5207 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 2027 3737 2027 Size: 1710 3898 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI