Restriction map for
pFN21AE3010
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccIII AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstF5I BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DpnII
DraI DraIII EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FokI FseI HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SapI Sau3AI
SbfI ScaI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 76 GTCGAC GT^[AC][GT]AC
AccII 266 CGCG CG^CG
AclI 228 AACGTT AA^CGTT
AluI 131 AGCT AG^CT
ApaI 276 GGGCCC GGGCC^C
AvaI 97 CCCGAG C^[CT]CG[GA]G
AvaII 249 GGACC G^G[AT]CC
AviII 11 TGCGCA TGC^GCA
AvrII 63 CCTAGG C^CTAGG
BanII 276 GGGCCC G[GA]GC[CT]^C
BbsI 112 GAAGAC GAAGAC(2/6)
BbvI 183 GCAGC GCAGC(8/12)
BfaI 64 CTAG C^TAG
BlnI 63 CCTAGG C^CTAGG
BmyI 276 GGGCCC G[AGT]GC[ACT]^C
BpuAI 112 GAAGAC GAAGAC(2/6)
BseRI 127 GAGGAG GAGGAG(10/8)
BsiEI 268 CGGTCG CG[GA][CT]^CG
BsmAI 200 GAGAC GTCTC(1/5)
BsmBI 200 GAGACG CGTCTC(1/5)
BsoBI 97 CCCGAG C^[CT]CG[GA]G
Bsp1286I 276 GGGCCC G[AGT]GC[ACT]^C
BstEII 174 GGTCACC G^GT[ACGT]ACC
BstNI 171 CCTGG CC^[AT]GG
BstPI 174 GGTCACC G^GT[ACGT]ACC
BstUI 266 CGCG CG^CG
BstXI 280 CCAGACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 275 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 250 GACCGCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 19 CCGTGG C^C[GA][CT]GG
EcoO109I 275 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 174 GGTCACC G^GT[ACGT]ACC
EcoRII 171 CCTGG ^CC[AT]GG
EcoT14I 63 CCTAGG C^C[AT][AT]GG
FauI 25 CCCGC CCCGC(4/6)
Fnu4HI 183 GCAGC GC^[ACGT]GC
FspI 11 TGCGCA TGC^GCA
HaeII 69 GGCGCT [GA]GCGC^[CT]
HincII 76 GTCGAC GT[CT]^[GA]AC
HindII 76 GTCGAC GT[CT]^[GA]AC
HphI 176 TCACC GGTGA(8/7)
ItaI 183 GCAGC GC^[ACGT]GC
MaeI 64 CTAG C^TAG
MaeII 229 ACGT A^CGT
MaeIII 175 GTCAC ^GT[ACGT]AC
MspA1I 184 CAGCGG C[AC]G^C[GT]G
MvaI 171 CCTGG CC^[AT]GG
MvnI 266 CGCG CG^CG
Psp1406I 228 AACGTT AA^CGTT
SalI 76 GTCGAC G^TCGAC
SexAI 170 ACCTGGT A^CC[AT]GGT
StyI 63 CCTAGG C^C[AT][AT]GG
TaqI 77 TCGA T^CGA
TseI 183 GCAGC G^C[AT]GC
Tsp45I 175 GTCAC ^GT[GC]AC
TspRI 38 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 77 TCGA T^CGA
XcmI 35 CCACAGTGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
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AcyI 79 GACGCC G[GA]^CG[CT]C
115 GACGCC
BsaHI 79 GACGCC G[GA]^CG[CT]C
115 GACGCC
BsiYI 165 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
179 CCCGGCAGCGG
BslI 165 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
179 CCCGGCAGCGG
Cac8I 14 GCAGGC GC[ACGT]^[ACGT]GC
24 GCCCGC
HgaI 79 GACGC GACGC(5/10)
115 GACGC
Hin1I 79 GACGCC G[GA]^CG[CT]C
115 GACGCC
MboII 112 GAAGA GAAGA(8/7)
233 TCTTC
MwoI 123 GCCGGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
245 GCGAGGACCGC
NlaIV 102 GGCTCC GG[ACGT]^[ACGT]CC
276 GGGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI BcnI BsaJI BssKI CfoI
Cfr13I HaeIII HapII HhaI HinP1I
HpaII MnlI MspI NciI Sau96I
ScrFI