################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AlwI AocI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 1025 GAATTC [GA]^AATT[CT] Alw44I 650 GTGCAC G^TGCAC Aor51HI 1280 AGCGCT AGC^GCT ApaLI 650 GTGCAC G^TGCAC ApoI 1025 GAATTC [GA]^AATT[CT] AseI 948 ATTAAT AT^TAAT AsnI 948 ATTAAT AT^TAAT BbsI 290 GTCTTC GAAGAC(2/6) BclI 1010 TGATCA T^GATCA BpuAI 290 GTCTTC GAAGAC(2/6) BseRI 3 GAGGAG GAGGAG(10/8) BsgI 612 CTGCAC GTGCAG(16/14) BsmFI 8 GGGAC GGGAC(10/14) BspMI 32 GCAGGT ACCTGC(4/8) BsrBI 1144 GAGCGG CCGCTC(-3/-3) DpnII 1011 GATC ^GATC DsaI 817 CCACGG C^C[GA][CT]GG Eco47III 1280 AGCGCT AGC^GCT EcoRI 1025 GAATTC G^AATTC EcoT14I 548 CCAAGG C^C[AT][AT]GG FbaI 1010 TGATCA T^GATCA HaeII 1280 AGCGCT [GA]GCGC^[CT] HgaI 394 GACGC GACGC(5/10) MaeII 11 ACGT A^CGT MboI 1011 GATC ^GATC NdeII 1011 GATC ^GATC PflMI 155 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1201 AGGACCC [GA]G^G[AT]CC[CT] PshBI 948 ATTAAT AT^TAAT PvuII 1063 CAGCTG CAG^CTG SapI 1125 GAAGAGC GCTCTTC(1/4) Sau3AI 1011 GATC ^GATC ScaI 360 AGTACT AGT^ACT SpeI 567 ACTAGT A^CTAGT StuI 593 AGGCCT AGG^CCT StyI 548 CCAAGG C^C[AT][AT]GG TaqI 975 TCGA T^CGA TthHB8I 975 TCGA T^CGA Van91I 155 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 117 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 138 CCGC CCGC(-3/-1) 1146 GCGG AflIII 400 ACATGT A^C[GA][CT]GT 473 ACATGT AvaII 912 GGACC G^G[AT]CC 1202 GGACC BanI 437 GGCACC G^G[CT][GA]CC 734 GGTGCC BsmI 795 GAATGC GAATGC(1/-1) 557 GCATTC Bsp1407I 379 TGTACA T^GTACA 457 TGTACA BspLU11I 400 ACATGT A^CATGT 473 ACATGT BsrGI 379 TGTACA T^GTACA 457 TGTACA BsrI 191 ACTGG ACTGG(1/-1) 666 ACTGG BstAPI 728 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1289 GCAGATGCTGC CfoI 1052 GCGC GCG^C 1281 GCGC EarI 919 GAAGAG CTCTTC(1/4) 1125 GAAGAG EcoT22I 320 ATGCAT ATGCA^T 1106 ATGCAT HhaI 1052 GCGC GCG^C 1281 GCGC HinP1I 1052 GCGC G^CGC 1281 GCGC Ksp632I 919 GAAGAG CTCTTC(1/4) 1125 GAAGAG MspA1I 138 CCGCTG C[AC]G^C[GT]G 1063 CAGCTG NsiI 320 ATGCAT ATGCA^T 1106 ATGCAT NspI 400 ACATGT [GA]CATG^[CT] 473 ACATGT PleI 1196 GACTC GAGTC(4/5) 990 GAGTC PstI 345 CTGCAG CTGCA^G 1287 CTGCAG SfcI 345 CTGCAG C^T[GA][CT]AG 1287 CTGCAG SspBI 379 TGTACA T^GTACA 457 TGTACA TfiI 366 GAATC G^A[AT]TC 1187 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AfaI AluI AlwNI AspHI BbvI BfaI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SmlI SspI Tru9I TseI Tsp45I Tsp509I TspRI