(Circular) (Six-base) MAPSORT of: pFN21AE3972.seq Check: 3217 from: 1 to: 6013 pFN21AE3972 6013 bp With 182 enzymes: SgfI * March 28, 2012 11:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3356 1937 Size: 1419 4594 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4076 5460 5728 1632 Size: 2444 1384 268 1917 Fragments arranged by size: 2444 1917 1384 268 AfeI AGC'GCT Cuts at: 3287 3287 Size: 6013 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 2405 2478 4912 5889 1203 Size: 1202 73 2434 977 1327 Fragments arranged by size: 2434 1327 1202 977 73 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2365 1184 Size: 1181 4832 AlwNI CAG_nnn'CTG Cuts at: 2166 2196 3300 3756 5328 2166 Size: 30 1104 456 1572 2851 Fragments arranged by size: 2851 1572 1104 456 30 ApaLI G'TGCA_C Cuts at: 2655 4070 5226 2655 Size: 1415 1156 3442 Fragments arranged by size: 3442 1415 1156 ApoI r'AATT_y Cuts at: 1493 3030 3322 3548 3700 5809 1493 Size: 1537 292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1537 292 226 152 AseI AT'TA_AT Cuts at: 160 1032 2954 4567 160 Size: 872 1922 1613 1606 Fragments arranged by size: 1922 1613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 3338 1870 Size: 73 1395 4545 Fragments arranged by size: 4545 1395 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2442 2739 3334 4789 618 Size: 325 217 627 655 297 595 1455 1842 Fragments arranged by size: 1842 1455 655 627 595 325 297 217 BanII G_rGCy'C Cuts at: 1391 3332 1391 Size: 1941 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 928 Size: 971 389 4653 Fragments arranged by size: 4653 971 389 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 3015 1548 Size: 1467 4546 BfrBI ATG'CAT Cuts at: 2327 3113 2327 Size: 786 5227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6008 6008 Size: 6013 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2659 4074 5230 1790 Size: 869 1415 1156 2573 Fragments arranged by size: 2573 1415 1156 869 BmgBI CAC'GTC Cuts at: 1318 2017 1318 Size: 699 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3744 4702 442 Size: 416 53 83 399 24 2327 958 1753 Fragments arranged by size: 2327 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 3018 2068 Size: 950 5063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 3092 3140 4673 1239 Size: 904 949 48 1533 2579 Fragments arranged by size: 2579 1533 949 904 48 Bpu10I CC'TnA_GC Cuts at: 2151 2417 2151 Size: 266 5747 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2579 2627 3167 4138 5003 5301 2579 Size: 48 540 971 865 298 3291 Fragments arranged by size: 3291 971 865 540 298 48 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2161 2597 2723 4676 882 Size: 617 662 436 126 1953 2219 Fragments arranged by size: 2219 1953 662 617 436 126 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3820 4202 4837 275 Size: 53 83 186 936 2287 382 635 1451 Fragments arranged by size: 2287 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5788 Fragments arranged by size: 5788 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 1186 Size: 837 5176 BseYI C'CCAG_C Cuts at: 5216 5712 5216 Size: 496 5517 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2602 1163 Size: 573 866 4574 Fragments arranged by size: 4574 866 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 2659 3126 4074 4159 5230 2152 Size: 507 467 948 85 1071 2935 Fragments arranged by size: 2935 1071 948 507 467 85 BsmI GAATG_Cn' Cuts at: 2562 2806 3524 3617 2562 Size: 244 718 93 4958 Fragments arranged by size: 4958 718 244 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2659 3126 3332 4074 4159 5230 Size: 399 362 507 467 206 742 85 1071 Cuts at: 5230 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 507 467 399 362 206 85 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3902 5632 3902 Size: 1730 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 3368 844 Size: 1184 1340 3489 Fragments arranged by size: 3489 1340 1184 BsrBI CCG'CTC Cuts at: 1394 1823 3151 3900 1394 Size: 429 1328 749 3507 Fragments arranged by size: 3507 1328 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4844 1700 Size: 110 2847 187 2869 Fragments arranged by size: 2869 2847 187 110 BsrGI T'GTAC_A Cuts at: 96 2384 2462 96 Size: 2288 78 3647 Fragments arranged by size: 3647 2288 78 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2739 3300 794 Size: 1945 561 3507 Fragments arranged by size: 3507 1945 561 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstYI r'GATC_y Cuts at: 1972 3343 3710 4095 4112 5564 5650 5662 6008 Size: 1371 367 385 17 1452 86 12 346 Cuts at: 6008 1972 Size: 1977 Fragments arranged by size: 1977 1452 1371 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2822 513 Size: 552 1757 3704 Fragments arranged by size: 3704 1757 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 2659 3529 4323 4343 2659 Size: 870 794 20 4329 Fragments arranged by size: 4329 870 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978 Size: 54 1425 50 275 18 1630 889 1629 Cuts at: 5978 8 Size: 43 Fragments arranged by size: 1630 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2919 3125 3943 1353 Size: 1566 206 818 3423 Fragments arranged by size: 3423 1566 818 206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 3092 3140 4076 4673 5460 5728 Size: 393 511 949 48 936 597 787 268 Cuts at: 5728 1239 Size: 1524 Fragments arranged by size: 1524 949 936 787 597 511 393 268 48 EcoO109I rG'GnC_Cy Cuts at: 2163 3051 3207 2163 Size: 888 156 4969 Fragments arranged by size: 4969 888 156 EcoRI G'AATT_C Cuts at: 3030 3322 3700 3030 Size: 292 378 5343 Fragments arranged by size: 5343 378 292 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 3289 5160 1678 Size: 1611 1871 2531 Fragments arranged by size: 2531 1871 1611 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3288 3320 4654 4686 4728 Size: 32 135 32 1716 32 1334 32 42 Cuts at: 4728 4760 1373 Size: 32 2626 Fragments arranged by size: 2626 1716 1334 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 2802 2983 5127 5311 1305 Size: 1191 306 181 2144 184 2007 Fragments arranged by size: 2144 2007 1191 306 184 181 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3971 4330 4489 518 Size: 648 2805 359 159 2042 Fragments arranged by size: 2805 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2145 3070 3429 4106 5254 5499 1606 Size: 539 925 359 677 1148 245 2120 Fragments arranged by size: 2120 1148 925 677 539 359 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5461 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NsiI A_TGCA'T Cuts at: 2329 3115 2329 Size: 786 5227 NspI r_CATG'y Cuts at: 1132 1229 2409 2482 3371 4916 1132 Size: 97 1180 73 889 1545 2229 Fragments arranged by size: 2229 1545 1180 889 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 2405 2478 4912 2405 Size: 73 2434 3506 Fragments arranged by size: 3506 2434 73 PflMI CCAn_nnn'nTGG Cuts at: 1108 2166 1108 Size: 1058 4955 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3207 3207 Size: 6013 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2088 2120 2088 Size: 32 5981 PstI C_TGCA'G Cuts at: 830 2354 3296 3365 830 Size: 1524 942 69 3478 Fragments arranged by size: 3478 1524 942 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 PvuII CAG'CTG Cuts at: 3070 3070 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SapI GCTCTTCn'nnn_ Cuts at: 3125 3125 Size: 6013 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 2367 4261 5973 2367 Size: 1894 1712 2407 Fragments arranged by size: 2407 1894 1712 SfcI C'TryA_G Cuts at: 826 1045 2350 3292 3361 4496 5177 5368 826 Size: 219 1305 942 69 1135 681 191 1471 Fragments arranged by size: 1471 1305 1135 942 681 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2594 2642 3146 4117 5018 5280 Size: 197 926 651 48 504 971 901 262 Cuts at: 5280 820 Size: 1553 Fragments arranged by size: 1553 971 926 901 651 504 262 197 48 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 2572 152 Size: 2420 3593 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 2257 2835 3223 3937 5 Size: 47 2205 578 388 714 2081 Fragments arranged by size: 2205 2081 714 578 388 47 StuI AGG'CCT Cuts at: 1517 2600 1517 Size: 1083 4930 StyI C'CwwG_G Cuts at: 513 1065 2553 3452 513 Size: 552 1488 899 3074 Fragments arranged by size: 3074 1488 899 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2370 4040 4057 4210 4395 4840 325 Size: 1255 790 1670 17 153 185 445 1498 Fragments arranged by size: 1670 1498 1255 790 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2365 2384 2408 Size: 274 80 33 51 1443 388 19 24 Cuts at: 2408 2462 2646 4259 5971 96 Size: 54 184 1613 1712 138 Fragments arranged by size: 1712 1613 1443 388 274 184 138 80 54 51 33 24 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 2122 3349 2122 Size: 1227 4786 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 4142 4142 Size: 6013 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI