(Linear) (Six-base) MAPSORT of: KIEE3972.seq Check: 5307 from: 1 to: 1308 KIEE3972.orf With 182 enzymes: SgfI * March 22, 2012 17:01 .. AfeI AGC'GCT Cuts at: 0 1282 1308 Size: 1282 26 AflIII A'CryG_T Cuts at: 0 400 473 1308 Size: 400 73 835 Fragments arranged by size: 835 400 73 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 360 1308 Size: 360 948 AlwNI CAG_nnn'CTG Cuts at: 0 161 191 1295 1308 Size: 161 30 1104 13 Fragments arranged by size: 1104 161 30 13 ApaLI G'TGCA_C Cuts at: 0 650 1308 Size: 650 658 ApoI r'AATT_y Cuts at: 0 1025 1308 Size: 1025 283 AseI AT'TA_AT Cuts at: 0 949 1308 Size: 949 359 BanI G'GyrC_C Cuts at: 0 437 734 1308 Size: 437 297 574 Fragments arranged by size: 574 437 297 BbsI GAAGACnn'nnnn_ Cuts at: 0 283 1308 Size: 283 1025 BclI T'GATC_A Cuts at: 0 1010 1308 Size: 1010 298 BfrBI ATG'CAT Cuts at: 0 322 1108 1308 Size: 322 786 200 Fragments arranged by size: 786 322 200 Bme1580I G_kGCm'C Cuts at: 0 654 1308 Size: 654 654 BmgBI CAC'GTC Cuts at: 0 12 1308 Size: 12 1296 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 63 1013 1308 Size: 63 950 295 Fragments arranged by size: 950 295 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 1087 1135 1308 Size: 138 949 48 173 Fragments arranged by size: 949 173 138 48 Bpu10I CC'TnA_GC Cuts at: 0 146 412 1308 Size: 146 266 896 Fragments arranged by size: 896 266 146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 622 1162 1308 Size: 574 48 540 146 Fragments arranged by size: 574 540 146 48 BsaI GGTCTCn'nnnn_ Cuts at: 0 156 592 718 1308 Size: 156 436 126 590 Fragments arranged by size: 590 436 156 126 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 18 1308 Size: 18 1290 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 597 1308 Size: 597 711 BsiHKAI G_wGCw'C Cuts at: 0 147 654 1121 1308 Size: 147 507 467 187 Fragments arranged by size: 507 467 187 147 BsmI GAATG_Cn' Cuts at: 0 557 801 1308 Size: 557 244 507 Fragments arranged by size: 557 507 244 Bsp1286I G_dGCh'C Cuts at: 0 147 654 1121 1308 Size: 147 507 467 187 Fragments arranged by size: 507 467 187 147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 23 1308 Size: 23 1285 BsrBI CCG'CTC Cuts at: 0 1146 1308 Size: 1146 162 BsrGI T'GTAC_A Cuts at: 0 379 457 1308 Size: 379 78 851 Fragments arranged by size: 851 379 78 BstAPI GCAn_nnn'nTGC Cuts at: 0 734 1295 1308 Size: 734 561 13 Fragments arranged by size: 734 561 13 BtgI C'CryG_G Cuts at: 0 817 1308 Size: 817 491 BtsI GCAGTG_nn' Cuts at: 0 654 1308 Size: 654 654 EarI CTCTTCn'nnn_ Cuts at: 0 914 1120 1308 Size: 914 206 188 Fragments arranged by size: 914 206 188 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 1087 1135 1308 Size: 138 949 48 173 Fragments arranged by size: 949 173 138 48 EcoO109I rG'GnC_Cy Cuts at: 0 158 1046 1202 1308 Size: 158 888 156 106 Fragments arranged by size: 888 158 156 106 EcoRI G'AATT_C Cuts at: 0 1025 1308 Size: 1025 283 HaeII r_GCGC'y Cuts at: 0 1284 1308 Size: 1284 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1283 1308 Size: 1283 25 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 491 797 978 1308 Size: 491 306 181 330 Fragments arranged by size: 491 330 306 181 MspA1I CmG'CkG Cuts at: 0 140 1065 1308 Size: 140 925 243 Fragments arranged by size: 925 243 140 NsiI A_TGCA'T Cuts at: 0 324 1110 1308 Size: 324 786 198 Fragments arranged by size: 786 324 198 NspI r_CATG'y Cuts at: 0 404 477 1308 Size: 404 73 831 Fragments arranged by size: 831 404 73 PciI A'CATG_T Cuts at: 0 400 473 1308 Size: 400 73 835 Fragments arranged by size: 835 400 73 PflMI CCAn_nnn'nTGG Cuts at: 0 161 1308 Size: 161 1147 PpuMI rG'GwC_Cy Cuts at: 0 1202 1308 Size: 1202 106 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 83 115 1308 Size: 83 32 1193 Fragments arranged by size: 1193 83 32 PstI C_TGCA'G Cuts at: 0 349 1291 1308 Size: 349 942 17 Fragments arranged by size: 942 349 17 PvuII CAG'CTG Cuts at: 0 1065 1308 Size: 1065 243 SapI GCTCTTCn'nnn_ Cuts at: 0 1120 1308 Size: 1120 188 ScaI AGT'ACT Cuts at: 0 362 1308 Size: 362 946 SfcI C'TryA_G Cuts at: 0 345 1287 1308 Size: 345 942 21 Fragments arranged by size: 942 345 21 SmlI C'TyrA_G Cuts at: 0 589 637 1141 1308 Size: 589 48 504 167 Fragments arranged by size: 589 504 167 48 SpeI A'CTAG_T Cuts at: 0 567 1308 Size: 567 741 SspI AAT'ATT Cuts at: 0 252 830 1218 1308 Size: 252 578 388 90 Fragments arranged by size: 578 388 252 90 StuI AGG'CCT Cuts at: 0 595 1308 Size: 595 713 StyI C'CwwG_G Cuts at: 0 548 1308 Size: 548 760 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 365 1308 Size: 365 943 TatI w'GTAC_w Cuts at: 0 360 379 403 457 641 1308 Size: 360 19 24 54 184 667 Fragments arranged by size: 667 360 184 54 24 19 XbaI T'CTAG_A Cuts at: 0 117 1308 Size: 117 1191 Enzymes that do cut: AfeI AflIII AlfI AlwNI ApaLI ApoI AseI BanI BbsI BclI BfrBI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrGI BstAPI BtgI BtsI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI MspA1I NsiI NspI PciI PflMI PpuMI PsrI PstI PvuII SapI ScaI SfcI SmlI SpeI SspI StuI StyI TaqII TatI XbaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AleI AloI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI BmrI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TstI Tth111I XcmI XhoI XmaI XmnI ZraI