################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AclI AfaI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BalI BbrPI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 266 TCCGGA T^CCGGA AcyI 839 GGCGCC G[GA]^CG[CT]C AflIII 599 ACGTGT A^C[GA][CT]GT AlwNI 313 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 921 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 342 GGTCC G^G[AT]CC BbeI 839 GGCGCC GGCGC^C BbsI 1023 GTCTTC GAAGAC(2/6) BbvCI 977 CCTCAGC CCTCAGC(-5/-2) BcgI 4 GCATTGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1023 GTCTTC GAAGAC(2/6) BsaBI 711 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 839 GGCGCC G[GA]^CG[CT]C BseAI 266 TCCGGA T^CCGGA BsgI 799 GTGCAG GTGCAG(16/14) BsiEI 14 CGACCG CG[GA][CT]^CG BsmBI 873 CGTCTC CGTCTC(1/5) BsmFI 284 GGGAC GGGAC(10/14) BspEI 266 TCCGGA T^CCGGA BspHI 719 TCATGA T^CATGA BsrDI 5 CATTGC GCAATG(2/0) BsrFI 664 GCCGGC [GA]^CCGG[CT] BssSI 658 CTCGTG CACGAG(-5/-1) Cfr10I 664 GCCGGC [GA]^CCGG[CT] DraIII 324 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 890 GAATTC G^AATTC EcoT14I 388 CCTTGG C^C[AT][AT]GG HaeII 839 GGCGCC [GA]GCGC^[CT] Hin1I 839 GGCGCC G[GA]^CG[CT]C HinfI 862 GAGTC G^A[ACGT]TC KasI 839 GGCGCC G^GCGCC MaeIII 46 GTCAC ^GT[ACGT]AC MamI 711 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 266 TCCGGA T^CCGGA NaeI 664 GCCGGC GCC^GGC NarI 839 GGCGCC GG^CGCC NgoMIV 664 GCCGGC G^CCGGC PleI 862 GAGTC GAGTC(4/5) PshAI 857 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 719 TCATGA T^CATGA SfoI 839 GGCGCC GGC^GCC SmlI 859 CTTGAG C^T[CT][GA]AG StuI 803 AGGCCT AGG^CCT StyI 388 CCTTGG C^C[AT][AT]GG Tsp45I 46 GTCAC ^GT[GC]AC TspRI 609 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 843 CCAGCTGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 921 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 471 GAATTT [GA]^AATT[CT] 890 GAATTC AhdI 30 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 165 GACCCCGTGTC ApoI 471 GAATTT [GA]^AATT[CT] 890 GAATTC AspEI 30 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 165 GACCCCGTGTC BanI 129 GGTGCC G^G[CT][GA]CC 839 GGCGCC BglI 303 GCCCAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 400 GCCGGGGAGGC BsaWI 266 TCCGGA [AT]^CCGG[AT] 622 ACCGGA BseRI 820 CTCCTC GAGGAG(10/8) 97 GAGGAG BsmAI 173 GTCTC GTCTC(1/5) 874 GTCTC EaeI 272 TGGCCG [CT]^GGCC[GA] 662 TGGCCG Eam1105I 30 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 165 GACCCCGTGTC EcoNI 216 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 977 CCTCAGCCAGG MaeII 599 ACGT A^CGT 872 ACGT MslI 346 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 360 CATGGAGGTG PvuII 540 CAGCTG CAG^CTG 844 CAGCTG SexAI 125 ACCTGGT A^CC[AT]GGT 338 ACCAGGT TaqI 13 TCGA T^CGA 449 TCGA Tsp509I 472 AATT ^AATT 891 AATT TthHB8I 13 TCGA T^CGA 449 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AluI AlwI BamHI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BsiYI BslI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TseI XhoII