(Circular) (Six-base) MAPSORT of: pFN21AE2226.seq Check: 2334 from: 1 to: 5764 pFN21AE2226 5764 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3015 3827 5211 5479 1632 Size: 1383 812 1384 268 1917 Fragments arranged by size: 1917 1384 1383 812 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2604 4663 5640 1203 Size: 1401 2059 977 1327 Fragments arranged by size: 2059 1401 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 2040 2175 4493 2040 Size: 135 2318 3311 Fragments arranged by size: 3311 2318 135 AleI CACnn'nnGTG Cuts at: 1166 2355 1166 Size: 1189 4575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 2323 3507 5079 2323 Size: 1184 1572 3008 Fragments arranged by size: 3008 1572 1184 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2476 2895 3073 3299 3451 5560 1493 Size: 983 419 178 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 419 226 178 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 3089 1870 Size: 73 1146 4545 Fragments arranged by size: 4545 1146 73 BamHI G'GATC_C Cuts at: 2460 2467 2698 3094 3461 2460 Size: 7 231 396 367 4763 Fragments arranged by size: 4763 396 367 231 7 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 2844 3085 4540 618 Size: 325 217 627 347 710 241 1455 1842 Fragments arranged by size: 1842 1455 710 627 347 325 241 217 BanII G_rGCy'C Cuts at: 1391 2378 2420 2786 3003 3083 1391 Size: 987 42 366 217 80 4072 Fragments arranged by size: 4072 987 366 217 80 42 BbeI G_GCGC'C Cuts at: 2848 2848 Size: 5764 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3021 928 Size: 971 1122 3671 Fragments arranged by size: 3671 1122 971 BbvCI CC'TCA_GC Cuts at: 2983 2983 Size: 5764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1998 2032 3955 3989 1903 Size: 34 61 34 1923 34 3678 Fragments arranged by size: 3678 1923 61 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2314 2411 4375 Size: 107 122 71 631 605 642 97 1964 Cuts at: 4375 136 Size: 1525 Fragments arranged by size: 1964 1525 642 631 605 122 107 97 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 2108 3002 3012 3181 2108 Size: 894 10 169 4691 Fragments arranged by size: 4691 894 169 10 Bme1580I G_kGCm'C Cuts at: 1790 3825 4981 1790 Size: 2035 1156 2573 Fragments arranged by size: 2573 2035 1156 BmgBI CAC'GTC Cuts at: 1318 2605 1318 Size: 1287 4477 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2698 3495 4453 Size: 416 53 83 399 24 1281 797 958 Cuts at: 4453 442 Size: 1753 Fragments arranged by size: 1753 1281 958 797 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2210 2544 4424 1239 Size: 932 39 334 1880 2579 Fragments arranged by size: 2579 1880 932 334 39 Bpu10I CC'TnA_GC Cuts at: 2785 2983 2785 Size: 198 5566 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2885 3889 4754 5052 2885 Size: 1004 865 298 3597 Fragments arranged by size: 3597 1004 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3555 493 Size: 711 2351 2702 Fragments arranged by size: 2702 2351 711 BsaBI GATnn'nnATC Cuts at: 2720 5758 2720 Size: 3038 2726 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2845 3571 3953 4588 Size: 53 83 186 936 1312 726 382 635 Cuts at: 4588 275 Size: 1451 Fragments arranged by size: 1451 1312 936 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 2627 4197 4595 4869 5016 5427 2271 Size: 356 1570 398 274 147 411 2608 Fragments arranged by size: 2608 1570 411 398 356 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2594 2624 1178 Size: 30 165 30 741 30 420 30 4318 Fragments arranged by size: 4318 741 420 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2816 1186 Size: 931 699 4134 Fragments arranged by size: 4134 931 699 BseYI C'CCAG_C Cuts at: 2240 2433 2649 2770 4967 5463 2240 Size: 193 216 121 2197 496 2541 Fragments arranged by size: 2541 2197 496 216 193 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 4102 Fragments arranged by size: 4102 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 3214 3975 5003 1540 Size: 275 186 21 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 21 BsiHKAI G_wGCw'C Cuts at: 3825 3910 4981 3825 Size: 85 1071 4608 Fragments arranged by size: 4608 1071 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 BsmBI CGTCTCn'nnnn_ Cuts at: 2884 2884 Size: 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2378 2420 2786 3003 3083 3825 3910 Size: 399 588 42 366 217 80 742 85 Cuts at: 3910 4981 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 588 399 366 217 85 80 42 BspEI T'CCGG_A Cuts at: 2271 5427 2271 Size: 3156 2608 BspHI T'CATG_A Cuts at: 2724 3653 5383 2724 Size: 929 1730 3105 Fragments arranged by size: 3105 1730 929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3119 844 Size: 2275 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2089 2205 2554 3651 1394 Size: 429 266 116 349 1097 3507 Fragments arranged by size: 3507 1097 429 349 266 116 BsrDI GCAATG_nn' Cuts at: 65 1248 2009 4259 4433 65 Size: 1183 761 2250 174 1396 Fragments arranged by size: 2250 1396 1183 761 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2669 4408 4595 1700 Size: 110 859 1739 187 2869 Fragments arranged by size: 2869 1739 859 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2663 3824 4836 2663 Size: 1161 1012 3591 Fragments arranged by size: 3591 1161 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstYI r'GATC_y Cuts at: 1972 2460 2467 2698 2715 3094 3461 3846 3863 Size: 488 7 231 17 379 367 385 17 Cuts at: 3863 5315 5401 5413 5759 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 488 385 379 367 346 231 86 17 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 3280 4074 4094 3280 Size: 794 20 4950 Fragments arranged by size: 4950 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DraIII CAC_nnn'GTG Cuts at: 2334 2334 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2667 3211 Size: 54 1425 50 275 18 447 390 544 Cuts at: 3211 4100 5729 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 544 447 390 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3694 1353 Size: 2341 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2210 2544 3015 3827 4424 5211 Size: 393 539 39 334 471 812 597 787 Cuts at: 5211 5479 1239 Size: 268 1524 Fragments arranged by size: 1524 812 787 597 539 471 393 334 268 39 EcoNI CCTnn'n_nnAGG Cuts at: 2225 2986 2225 Size: 761 5003 EcoRI G'AATT_C Cuts at: 2895 3073 3451 2895 Size: 178 378 5208 Fragments arranged by size: 5208 378 178 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2848 4911 1678 Size: 1170 2063 2531 Fragments arranged by size: 2531 2063 1170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2161 2193 2452 Size: 32 135 32 454 32 103 32 259 Cuts at: 2452 2484 2594 2626 2690 2722 4405 4437 4479 Size: 32 110 32 64 32 1683 32 42 Cuts at: 4479 4511 1373 Size: 32 2626 Fragments arranged by size: 2626 1683 454 259 135 110 103 64 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KasI G'GCGC_C Cuts at: 2844 2844 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4878 5062 1305 Size: 3573 184 2007 Fragments arranged by size: 3573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 2369 3722 4081 4240 518 Size: 648 1189 14 1353 359 159 2042 Fragments arranged by size: 2042 1353 1189 648 359 159 14 MspA1I CmG'CkG Cuts at: 1606 2380 2547 2851 3180 3857 5005 5250 1606 Size: 774 167 304 329 677 1148 245 2120 Fragments arranged by size: 2120 1148 774 677 329 304 245 167 NaeI GCC'GGC Cuts at: 2671 2671 Size: 5764 NarI GG'CG_CC Cuts at: 2845 2845 Size: 5764 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NgoMIV G'CCGG_C Cuts at: 2669 2669 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 3020 1348 Size: 1672 4092 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5764 PfoI T'CCnGG_A Cuts at: 2463 2857 2463 Size: 394 5370 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2866 1613 Size: 1253 4511 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PstI C_TGCA'G Cuts at: 830 2193 2250 2893 3116 830 Size: 1363 57 643 223 3478 Fragments arranged by size: 3478 1363 643 223 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2547 2851 2547 Size: 304 5460 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SexAI A'CCwGG_T Cuts at: 2130 2343 2130 Size: 213 5551 SfcI C'TryA_G Cuts at: 826 1045 2189 2246 2889 3112 4247 4928 5119 Size: 219 1144 57 643 223 1135 681 191 Cuts at: 5119 826 Size: 1471 Fragments arranged by size: 1471 1144 1135 681 643 223 219 191 57 SfoI GGC'GCC Cuts at: 2846 2846 Size: 5764 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2864 3868 4769 5031 820 Size: 197 926 921 1004 901 262 1553 Fragments arranged by size: 1553 1004 926 921 901 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 2810 1517 Size: 1293 4471 StyI C'CwwG_G Cuts at: 513 1065 2004 2393 3203 513 Size: 552 939 389 810 3074 Fragments arranged by size: 3074 939 810 552 389 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3033 3791 3808 3961 4146 4591 325 Size: 1255 1453 758 17 153 185 445 1498 Fragments arranged by size: 1498 1453 1255 758 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 2683 2715 5724 5756 2318 Size: 32 333 32 3009 32 2326 Fragments arranged by size: 3009 2326 333 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2855 2855 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 2930 3893 2930 Size: 963 4801 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI