(Linear) (Six-base) MAPSORT of: KIEE2226.seq Check: 8936 from: 1 to: 1059 KIEE2226.orf With 182 enzymes: SgfI * July 3, 2012 17:16 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1010 1059 Size: 1010 49 AflIII A'CryG_T Cuts at: 0 599 1059 Size: 599 460 AhdI GACnn_n'nnGTC Cuts at: 0 35 170 1059 Size: 35 135 889 Fragments arranged by size: 889 135 35 AleI CACnn'nnGTG Cuts at: 0 350 1059 Size: 350 709 AlwNI CAG_nnn'CTG Cuts at: 0 318 1059 Size: 318 741 ApoI r'AATT_y Cuts at: 0 471 890 1059 Size: 471 419 169 Fragments arranged by size: 471 419 169 BamHI G'GATC_C Cuts at: 0 455 462 693 1059 Size: 455 7 231 366 Fragments arranged by size: 455 366 231 7 BanI G'GyrC_C Cuts at: 0 129 839 1059 Size: 129 710 220 Fragments arranged by size: 710 220 129 BanII G_rGCy'C Cuts at: 0 373 415 781 998 1059 Size: 373 42 366 217 61 Fragments arranged by size: 373 366 217 61 42 BbeI G_GCGC'C Cuts at: 0 843 1059 Size: 843 216 BbsI GAAGACnn'nnnn_ Cuts at: 0 1016 1059 Size: 1016 43 BbvCI CC'TCA_GC Cuts at: 0 978 1059 Size: 978 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 27 1059 Size: 27 1032 BglI GCCn_nnn'nGGC Cuts at: 0 309 406 1059 Size: 309 97 653 Fragments arranged by size: 653 309 97 BlpI GC'TnA_GC Cuts at: 0 103 997 1007 1059 Size: 103 894 10 52 Fragments arranged by size: 894 103 52 10 BmgBI CAC'GTC Cuts at: 0 600 1059 Size: 600 459 BmrI ACTGGGnnnn_n' Cuts at: 0 693 1059 Size: 693 366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 205 539 1059 Size: 166 39 334 520 Fragments arranged by size: 520 334 166 39 Bpu10I CC'TnA_GC Cuts at: 0 780 978 1059 Size: 780 198 81 Fragments arranged by size: 780 198 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 880 1059 Size: 880 179 BsaBI GATnn'nnATC Cuts at: 0 715 1059 Size: 715 344 BsaHI Gr'CG_yC Cuts at: 0 840 1059 Size: 840 219 BsaWI w'CCGG_w Cuts at: 0 266 622 1059 Size: 266 356 437 Fragments arranged by size: 437 356 266 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 139 169 589 619 1059 Size: 139 30 420 30 440 Fragments arranged by size: 440 420 139 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 811 1059 Size: 112 699 248 Fragments arranged by size: 699 248 112 BseYI C'CCAG_C Cuts at: 0 235 428 644 765 1059 Size: 235 193 216 121 294 Fragments arranged by size: 294 235 216 193 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 820 1059 Size: 820 239 BsiEI CG_ry'CG Cuts at: 0 17 1059 Size: 17 1042 BsmBI CGTCTCn'nnnn_ Cuts at: 0 879 1059 Size: 879 180 Bsp1286I G_dGCh'C Cuts at: 0 373 415 781 998 1059 Size: 373 42 366 217 61 Fragments arranged by size: 373 366 217 61 42 BspEI T'CCGG_A Cuts at: 0 266 1059 Size: 266 793 BspHI T'CATG_A Cuts at: 0 719 1059 Size: 719 340 BsrBI CCG'CTC Cuts at: 0 84 200 549 1059 Size: 84 116 349 510 Fragments arranged by size: 510 349 116 84 BsrDI GCAATG_nn' Cuts at: 0 4 1059 Size: 4 1055 BsrFI r'CCGG_y Cuts at: 0 664 1059 Size: 664 395 BssSI C'ACGA_G Cuts at: 0 658 1059 Size: 658 401 BstYI r'GATC_y Cuts at: 0 455 462 693 710 1059 Size: 455 7 231 17 349 Fragments arranged by size: 455 349 231 17 7 DraIII CAC_nnn'GTG Cuts at: 0 329 1059 Size: 329 730 EaeI y'GGCC_r Cuts at: 0 272 662 1059 Size: 272 390 397 Fragments arranged by size: 397 390 272 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 205 539 1010 1059 Size: 166 39 334 471 49 Fragments arranged by size: 471 334 166 49 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 220 981 1059 Size: 220 761 78 Fragments arranged by size: 761 220 78 EcoRI G'AATT_C Cuts at: 0 890 1059 Size: 890 169 HaeII r_GCGC'y Cuts at: 0 843 1059 Size: 843 216 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 21 53 156 188 447 479 589 621 Size: 21 32 103 32 259 32 110 32 Cuts at: 621 685 717 1059 Size: 64 32 342 Fragments arranged by size: 342 259 110 103 64 32 32 32 32 32 21 KasI G'GCGC_C Cuts at: 0 839 1059 Size: 839 220 MslI CAynn'nnrTG Cuts at: 0 350 364 1059 Size: 350 14 695 Fragments arranged by size: 695 350 14 MspA1I CmG'CkG Cuts at: 0 375 542 846 1059 Size: 375 167 304 213 Fragments arranged by size: 375 304 213 167 NaeI GCC'GGC Cuts at: 0 666 1059 Size: 666 393 NarI GG'CG_CC Cuts at: 0 840 1059 Size: 840 219 NgoMIV G'CCGG_C Cuts at: 0 664 1059 Size: 664 395 PasI CC'CwG_GG Cuts at: 0 1015 1059 Size: 1015 44 PfoI T'CCnGG_A Cuts at: 0 458 852 1059 Size: 458 394 207 Fragments arranged by size: 458 394 207 PshAI GACnn'nnGTC Cuts at: 0 861 1059 Size: 861 198 PstI C_TGCA'G Cuts at: 0 188 245 888 1059 Size: 188 57 643 171 Fragments arranged by size: 643 188 171 57 PvuII CAG'CTG Cuts at: 0 542 846 1059 Size: 542 304 213 Fragments arranged by size: 542 304 213 SexAI A'CCwGG_T Cuts at: 0 125 338 1059 Size: 125 213 721 Fragments arranged by size: 721 213 125 SfcI C'TryA_G Cuts at: 0 184 241 884 1059 Size: 184 57 643 175 Fragments arranged by size: 643 184 175 57 SfoI GGC'GCC Cuts at: 0 841 1059 Size: 841 218 SmlI C'TyrA_G Cuts at: 0 859 1059 Size: 859 200 StuI AGG'CCT Cuts at: 0 805 1059 Size: 805 254 StyI C'CwwG_G Cuts at: 0 388 1059 Size: 388 671 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1028 1059 Size: 1028 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 313 345 678 710 1059 Size: 313 32 333 32 349 Fragments arranged by size: 349 333 313 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 850 1059 Size: 850 209 XmnI GAAnn'nnTTC Cuts at: 0 925 1059 Size: 925 134 Enzymes that do cut: AcuI AflIII AhdI AleI AlwNI ApoI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssSI BstYI DraIII EaeI Eco57MI EcoNI EcoRI HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV PasI PfoI PshAI PstI PvuII SexAI SfcI SfoI SmlI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BciVI BclI BfrBI BglII Bme1580I BmtI BplI BsaI BsaAI BsiHKAI BsiWI BsmI BspMI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI ZraI