################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NarI NciI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 95 CCGC CCGC(-3/-1) AcsI 135 GAATTT [GA]^AATT[CT] ApoI 135 GAATTT [GA]^AATT[CT] AspHI 171 GTGCTC G[AT]GC[AT]^C BmyI 171 GTGCTC G[AGT]GC[ACT]^C BpmI 265 CTGGAG CTGGAG(16/14) BsaI 6 GGTCTC GGTCTC(1/5) BsaJI 41 CCATGG C^C[ACGT][ACGT]GG BsiEI 92 CGACCG CG[GA][CT]^CG BsiHKAI 171 GTGCTC G[AT]GC[AT]^C BsiYI 228 CCCCAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 228 CCCCAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 157 GAGACG CGTCTC(1/5) Bsp1286I 171 GTGCTC G[AGT]GC[ACT]^C BsrFI 209 GCCGGC [GA]^CCGG[CT] Cfr10I 209 GCCGGC [GA]^CCGG[CT] DdeI 271 CTGAG C^T[ACGT]AG DraII 59 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 41 CCATGG C^C[GA][CT]GG EcoO109I 59 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 41 CCATGG C^C[AT][AT]GG HaeIII 208 GGCC GG^CC HapII 210 CCGG C^CGG HinfI 126 GATTC G^A[ACGT]TC HpaII 210 CCGG C^CGG MaeII 254 ACGT A^CGT MboII 191 TCTTC GAAGA(8/7) MspI 210 CCGG C^CGG NaeI 209 GCCGGC GCC^GGC NcoI 41 CCATGG C^CATGG NgoMIV 209 GCCGGC G^CCGGC NlaIII 42 CATG CATG^ NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC PpuMI 59 AGGTCCT [GA]G^G[AT]CC[CT] SexAI 56 ACCAGGT A^CC[AT]GGT StyI 41 CCATGG C^C[AT][AT]GG TfiI 126 GATTC G^A[AT]TC Tsp509I 136 AATT ^AATT ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 60 GGTCC G^G[AT]CC 243 GGTCC BseRI 31 CTCCTC GAGGAG(10/8) 235 GAGGAG BsgI 36 CTGCAC GTGCAG(16/14) 68 CTGCAC BsmAI 157 GAGAC GTCTC(1/5) 7 GTCTC BspMI 3 GCAGGT ACCTGC(4/8) 213 GCAGGT BssKI 57 CCAGG ^CC[ACGT]GG 264 CCTGG BstAPI 70 GCACCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 164 GCAGCCAGTGC BstNI 57 CCAGG CC^[AT]GG 264 CCTGG Cac8I 97 GCCTGC GC[ACGT]^[ACGT]GC 209 GCCGGC Cfr13I 60 GGTCC G^G[ACGT]CC 243 GGTCC EcoRII 57 CCAGG ^CC[AT]GG 264 CCTGG MvaI 57 CCAGG CC^[AT]GG 264 CCTGG Sau96I 60 GGTCC G^G[ACGT]CC 243 GGTCC ScrFI 57 CCAGG CC^[ACGT]GG 264 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AluI BbvI BsrI Fnu4HI ItaI MnlI MwoI TseI TspRI