(Circular) (Six-base) MAPSORT of: pFN21AE2015.seq Check: 3784 from: 1 to: 6481 pFN21AE2015 6481 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2741 3824 1937 Size: 804 1083 4594 Fragments arranged by size: 4594 1083 804 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2871 4544 5928 6196 1632 Size: 1239 1673 1384 268 1917 Fragments arranged by size: 1917 1673 1384 1239 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 5380 6357 1203 Size: 4177 977 1327 Fragments arranged by size: 4177 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 2359 5210 2359 Size: 2851 3630 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 2675 2687 4224 5796 2675 Size: 12 1537 1572 3360 Fragments arranged by size: 3360 1572 1537 12 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 2635 2924 2972 3124 3790 4016 4168 6277 Size: 1142 289 48 152 666 226 152 2109 Cuts at: 6277 1493 Size: 1697 Fragments arranged by size: 2109 1697 1142 666 289 226 152 152 48 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 3806 1870 Size: 73 1863 4545 Fragments arranged by size: 4545 1863 73 BamHI G'GATC_C Cuts at: 3811 4178 3811 Size: 367 6114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3802 5257 618 Size: 325 217 627 2015 1455 1842 Fragments arranged by size: 2015 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2234 3800 1391 Size: 843 1566 4072 Fragments arranged by size: 4072 1566 843 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2804 2971 928 Size: 971 905 167 4438 Fragments arranged by size: 4438 971 905 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4672 4706 1903 Size: 34 2735 34 3678 Fragments arranged by size: 3678 2735 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4373 5583 4373 Size: 1210 5271 BclI T'GATC_A Cuts at: 1548 2371 2410 3760 1548 Size: 823 39 1350 4269 Fragments arranged by size: 4269 1350 823 39 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3591 5092 136 Size: 107 122 71 631 605 1919 1501 1525 Fragments arranged by size: 1919 1525 1501 631 605 122 107 71 BglII A'GATC_T Cuts at: 6476 6476 Size: 6481 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 4542 5698 1790 Size: 2752 1156 2573 Fragments arranged by size: 2752 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4212 5170 442 Size: 416 53 83 399 24 2795 958 1753 Fragments arranged by size: 2795 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 5141 1239 Size: 910 2992 2579 Fragments arranged by size: 2992 2579 910 Bpu10I CC'TnA_GC Cuts at: 2892 2892 Size: 6481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4606 5471 5769 4606 Size: 865 298 5318 Fragments arranged by size: 5318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3051 3237 3417 3462 3486 3597 3627 Size: 617 1552 186 180 45 24 111 30 Cuts at: 3627 3678 3702 5144 882 Size: 51 24 1442 2219 Fragments arranged by size: 2219 1552 1442 617 186 180 111 51 45 30 24 24 BsaAI yAC'GTr Cuts at: 493 1204 4272 493 Size: 711 3068 2702 Fragments arranged by size: 3068 2702 711 BsaBI GATnn'nnATC Cuts at: 2376 6475 2376 Size: 4099 2382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4670 5305 275 Size: 53 83 186 936 2755 382 635 1451 Fragments arranged by size: 2755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2801 4914 5312 5586 5733 6144 2801 Size: 2113 398 274 147 411 3138 Fragments arranged by size: 3138 2113 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2723 2753 1178 Size: 30 165 30 1320 30 4906 Fragments arranged by size: 4906 1320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2565 2997 3192 3420 3435 3444 3459 3480 Size: 1379 432 195 228 15 9 15 21 Cuts at: 3480 3513 3525 3534 3546 3555 3567 3576 3588 Size: 33 12 9 12 9 12 9 12 Cuts at: 3588 3615 3669 3696 3720 1186 Size: 27 54 27 24 3947 Fragments arranged by size: 3947 1379 432 228 195 54 33 27 27 24 21 15 15 12 12 12 12 9 9 9 9 BseYI C'CCAG_C Cuts at: 5684 6180 5684 Size: 496 5985 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5908 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2234 2311 4542 4627 5698 2234 Size: 77 2231 85 1071 3017 Fragments arranged by size: 3017 2231 1071 85 77 BsmI GAATG_Cn' Cuts at: 3992 4085 3992 Size: 93 6388 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2311 3800 4542 4627 5698 1391 Size: 399 444 77 1489 742 85 1071 2174 Fragments arranged by size: 2174 1489 1071 742 444 399 85 77 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 2976 4370 6100 2976 Size: 1394 1730 3357 Fragments arranged by size: 3357 1730 1394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3836 844 Size: 2992 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3148 4976 5150 65 Size: 1183 1900 1828 174 1396 Fragments arranged by size: 1900 1828 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5125 5312 1700 Size: 110 3315 187 2869 Fragments arranged by size: 3315 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 3138 794 Size: 2344 4137 BstBI TT'CG_AA Cuts at: 5285 5285 Size: 6481 BstEII G'GTnAC_C Cuts at: 1184 2615 1184 Size: 1431 5050 BstXI CCAn_nnnn'nTGG Cuts at: 2087 2087 Size: 6481 BstYI r'GATC_y Cuts at: 1972 3811 4178 4563 4580 6032 6118 6130 6476 Size: 1839 367 385 17 1452 86 12 346 Cuts at: 6476 1972 Size: 1977 Fragments arranged by size: 1977 1839 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3172 3172 Size: 6481 BtgI C'CryG_G Cuts at: 513 1065 2620 2929 3013 3607 513 Size: 552 1555 309 84 594 3387 Fragments arranged by size: 3387 1555 594 552 309 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5742 Fragments arranged by size: 5742 683 56 BtsI GCAGTG_nn' Cuts at: 3625 3997 4791 4811 3625 Size: 372 794 20 5295 Fragments arranged by size: 5295 794 372 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 2767 3785 2767 Size: 1018 5463 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3928 4817 6446 Size: 54 1425 50 275 18 2098 889 1629 Cuts at: 6446 8 Size: 43 Fragments arranged by size: 2098 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2299 2581 4411 1353 Size: 946 282 1830 3423 Fragments arranged by size: 3423 1830 946 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2232 2232 Size: 6481 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2871 4544 5141 5928 6196 1239 Size: 393 517 722 1673 597 787 268 1524 Fragments arranged by size: 1673 1524 787 722 597 517 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2598 2598 Size: 6481 EcoO109I rG'GnC_Cy Cuts at: 2591 2591 Size: 6481 EcoRI G'AATT_C Cuts at: 2924 2972 3124 3790 4168 2924 Size: 48 152 666 378 5237 Fragments arranged by size: 5237 666 378 152 48 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2328 2328 Size: 6481 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 5628 1678 Size: 3950 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2384 2416 2669 Size: 32 135 32 773 32 7 32 253 Cuts at: 2669 2701 2723 2755 5122 5154 5196 5228 1373 Size: 32 22 32 2367 32 42 32 2626 Fragments arranged by size: 2626 2367 773 253 135 42 32 32 32 32 32 32 32 32 22 7 HincII GTy'rAC Cuts at: 669 1657 1938 2339 3825 4071 669 Size: 988 281 401 1486 246 3079 Fragments arranged by size: 3079 1486 988 401 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6481 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 5595 5779 1305 Size: 684 3606 184 2007 Fragments arranged by size: 3606 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4659 Fragments arranged by size: 4659 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2945 4439 4798 4957 518 Size: 648 1779 1494 359 159 2042 Fragments arranged by size: 2042 1779 1494 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2157 2622 3303 3318 3897 4574 5722 5967 Size: 551 465 681 15 579 677 1148 245 Cuts at: 5967 1606 Size: 2120 Fragments arranged by size: 2120 1148 681 677 579 551 465 245 15 NcoI C'CATG_G Cuts at: 513 1065 2929 513 Size: 552 1864 4065 Fragments arranged by size: 4065 1864 552 NdeI CA'TA_TG Cuts at: 387 2350 2389 387 Size: 1963 39 4479 Fragments arranged by size: 4479 1963 39 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 3839 5384 1132 Size: 97 2610 1545 2229 Fragments arranged by size: 2610 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 2333 2907 1108 Size: 1225 574 4682 Fragments arranged by size: 4682 1225 574 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2591 2591 Size: 6481 PshAI GACnn'nnGTC Cuts at: 1613 2334 3165 1613 Size: 721 831 4929 Fragments arranged by size: 4929 831 721 PsiI TTA'TAA Cuts at: 4051 4051 Size: 6481 PstI C_TGCA'G Cuts at: 830 3833 830 Size: 3003 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 SacI G_AGCT'C Cuts at: 2234 2234 Size: 6481 SacII CC_GC'GG Cuts at: 2623 2623 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SapI GCTCTTCn'nnn_ Cuts at: 2299 2299 Size: 6481 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SfcI C'TryA_G Cuts at: 826 1045 2152 2428 2470 2742 3217 3313 3751 Size: 219 1107 276 42 272 475 96 438 Cuts at: 3751 3829 4964 5645 5836 826 Size: 78 1135 681 191 1471 Fragments arranged by size: 1471 1135 1107 681 475 438 276 272 219 191 96 78 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4585 5486 5748 820 Size: 197 926 2642 901 262 1553 Fragments arranged by size: 2642 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 4405 5 Size: 47 4353 2081 Fragments arranged by size: 4353 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6481 StyI C'CwwG_G Cuts at: 513 1065 2080 2296 2929 3920 513 Size: 552 1015 216 633 991 3074 Fragments arranged by size: 3074 1015 991 633 552 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3261 3441 3486 3510 3651 3702 3726 Size: 1255 1681 180 45 24 141 51 24 Cuts at: 3726 4508 4525 4678 4863 5308 325 Size: 782 17 153 185 445 1498 Fragments arranged by size: 1681 1498 1255 782 445 185 180 153 141 51 45 24 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4727 6439 96 Size: 274 80 33 51 1443 2750 1712 138 Fragments arranged by size: 2750 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6441 6473 6441 Size: 32 6449 Tth111I GACn'n_nGTC Cuts at: 1316 2178 1316 Size: 862 5619 XbaI T'CTAG_A Cuts at: 3817 3817 Size: 6481 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2330 2330 Size: 6481 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 3124 4610 3124 Size: 1486 4995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI