(Linear) (Six-base) MAPSORT of: KIEE2015.seq Check: 3829 from: 1 to: 1776 KIEE2015.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AccI GT'mk_AC Cuts at: 0 736 1776 Size: 736 1040 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 866 1776 Size: 866 910 AhdI GACnn_n'nnGTC Cuts at: 0 354 1776 Size: 354 1422 AlwNI CAG_nnn'CTG Cuts at: 0 670 682 1776 Size: 670 12 1094 Fragments arranged by size: 1094 670 12 ApoI r'AATT_y Cuts at: 0 630 919 967 1119 1776 Size: 630 289 48 152 657 Fragments arranged by size: 657 630 289 152 48 BanII G_rGCy'C Cuts at: 0 229 1776 Size: 229 1547 BbsI GAAGACnn'nnnn_ Cuts at: 0 799 966 1776 Size: 799 167 810 Fragments arranged by size: 810 799 167 BclI T'GATC_A Cuts at: 0 366 405 1755 1776 Size: 366 39 1350 21 Fragments arranged by size: 1350 366 39 21 BglI GCCn_nnn'nGGC Cuts at: 0 1586 1776 Size: 1586 190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 1776 Size: 144 1632 Bpu10I CC'TnA_GC Cuts at: 0 887 1776 Size: 887 889 BsaI GGTCTCn'nnnn_ Cuts at: 0 1046 1232 1412 1457 1481 1592 1622 1673 Size: 1046 186 180 45 24 111 30 51 Cuts at: 1673 1697 1776 Size: 24 79 Fragments arranged by size: 1046 186 180 111 79 51 45 30 24 24 BsaBI GATnn'nnATC Cuts at: 0 371 1776 Size: 371 1405 BsaWI w'CCGG_w Cuts at: 0 796 1776 Size: 796 980 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 718 748 1776 Size: 718 30 1028 Fragments arranged by size: 1028 718 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 560 992 1187 1415 1430 1439 1454 1475 Size: 560 432 195 228 15 9 15 21 Cuts at: 1475 1508 1520 1529 1541 1550 1562 1571 1583 Size: 33 12 9 12 9 12 9 12 Cuts at: 1583 1610 1664 1691 1715 1776 Size: 27 54 27 24 61 Fragments arranged by size: 560 432 228 195 61 54 33 27 27 24 21 15 15 12 12 12 12 9 9 9 9 BsiHKAI G_wGCw'C Cuts at: 0 229 306 1776 Size: 229 77 1470 Fragments arranged by size: 1470 229 77 Bsp1286I G_dGCh'C Cuts at: 0 229 306 1776 Size: 229 77 1470 Fragments arranged by size: 1470 229 77 BspHI T'CATG_A Cuts at: 0 971 1776 Size: 971 805 BsrDI GCAATG_nn' Cuts at: 0 1143 1776 Size: 1143 633 BstAPI GCAn_nnn'nTGC Cuts at: 0 1133 1776 Size: 1133 643 BstEII G'GTnAC_C Cuts at: 0 610 1776 Size: 610 1166 BstXI CCAn_nnnn'nTGG Cuts at: 0 82 1776 Size: 82 1694 Bsu36I CC'TnA_GG Cuts at: 0 1167 1776 Size: 1167 609 BtgI C'CryG_G Cuts at: 0 615 924 1008 1602 1776 Size: 615 309 84 594 174 Fragments arranged by size: 615 594 309 174 84 BtsI GCAGTG_nn' Cuts at: 0 1620 1776 Size: 1620 156 DraI TTT'AAA Cuts at: 0 762 1776 Size: 762 1014 EarI CTCTTCn'nnn_ Cuts at: 0 294 576 1776 Size: 294 282 1200 Fragments arranged by size: 1200 294 282 EcoICRI GAG'CTC Cuts at: 0 227 1776 Size: 227 1549 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 866 1776 Size: 144 722 910 Fragments arranged by size: 910 722 144 EcoNI CCTnn'n_nnAGG Cuts at: 0 593 1776 Size: 593 1183 EcoO109I rG'GnC_Cy Cuts at: 0 586 1776 Size: 586 1190 EcoRI G'AATT_C Cuts at: 0 919 967 1119 1776 Size: 919 48 152 657 Fragments arranged by size: 919 657 152 48 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 323 1776 Size: 323 1453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 340 372 379 411 664 696 718 750 Size: 340 32 7 32 253 32 22 32 Cuts at: 750 1776 Size: 1026 Fragments arranged by size: 1026 340 253 32 32 32 32 22 7 HincII GTy'rAC Cuts at: 0 334 1776 Size: 334 1442 MslI CAynn'nnrTG Cuts at: 0 940 1776 Size: 940 836 MspA1I CmG'CkG Cuts at: 0 152 617 1298 1313 1776 Size: 152 465 681 15 463 Fragments arranged by size: 681 465 463 152 15 NcoI C'CATG_G Cuts at: 0 924 1776 Size: 924 852 NdeI CA'TA_TG Cuts at: 0 345 384 1776 Size: 345 39 1392 Fragments arranged by size: 1392 345 39 PflMI CCAn_nnn'nTGG Cuts at: 0 328 902 1776 Size: 328 574 874 Fragments arranged by size: 874 574 328 PpuMI rG'GwC_Cy Cuts at: 0 586 1776 Size: 586 1190 PshAI GACnn'nnGTC Cuts at: 0 329 1160 1776 Size: 329 831 616 Fragments arranged by size: 831 616 329 SacI G_AGCT'C Cuts at: 0 229 1776 Size: 229 1547 SacII CC_GC'GG Cuts at: 0 618 1776 Size: 618 1158 SapI GCTCTTCn'nnn_ Cuts at: 0 294 1776 Size: 294 1482 SfcI C'TryA_G Cuts at: 0 147 423 465 737 1212 1308 1746 1776 Size: 147 276 42 272 475 96 438 30 Fragments arranged by size: 475 438 276 272 147 96 42 30 StyI C'CwwG_G Cuts at: 0 75 291 924 1776 Size: 75 216 633 852 Fragments arranged by size: 852 633 216 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1256 1436 1481 1505 1646 1697 1721 1776 Size: 1256 180 45 24 141 51 24 55 Fragments arranged by size: 1256 180 141 55 51 45 24 24 Tth111I GACn'n_nGTC Cuts at: 0 173 1776 Size: 173 1603 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 325 1776 Size: 325 1451 XmnI GAAnn'nnTTC Cuts at: 0 1119 1776 Size: 1119 657 Enzymes that do cut: AccI AcuI AhdI AlwNI ApoI BanII BbsI BclI BglI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspHI BsrDI BstAPI BstEII BstXI Bsu36I BtgI BtsI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MslI MspA1I NcoI NdeI PflMI PpuMI PshAI SacI SacII SapI SfcI StyI TaqII Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI BsaHI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XhoI XmaI ZraI