Restriction map for
pFN21AE6469
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
BstBI BstXI Bsu36I Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV FbaI FseI FspI
HaeII HapII HgaI HpaII KasI
KpnI KspI MamI MluI MluNI
MroI MscI MspA1I MspI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 256 GACGTC GACGT^C
AccI 1056 GTATAC GT^[AC][GT]AC
AccII 41 CGCG CG^CG
AclI 700 AACGTT AA^CGTT
AcyI 256 GACGTC G[GA]^CG[CT]C
ApaI 44 GGGCCC GGGCC^C
AseI 874 ATTAAT AT^TAAT
AsnI 874 ATTAAT AT^TAAT
AspI 835 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 80 CCCGAG C^[CT]CG[GA]G
BbvCI 31 GCTGAGG CCTCAGC(-5/-2)
BglII 1011 AGATCT A^GATCT
BlpI 1402 GCTGAGC GC^T[ACGT]AGC
BpmI 1226 CTGGAG CTGGAG(16/14)
Bpu1102I 1402 GCTGAGC GC^T[ACGT]AGC
BsaHI 256 GACGTC G[GA]^CG[CT]C
BseRI 1090 GAGGAG GAGGAG(10/8)
BsmFI 1032 GGGAC GGGAC(10/14)
BsmI 1359 GAATGC GAATGC(1/-1)
BsoBI 80 CCCGAG C^[CT]CG[GA]G
Bsp1407I 323 TGTACA T^GTACA
BspMI 151 GCAGGT ACCTGC(4/8)
BsrGI 323 TGTACA T^GTACA
Bst1107I 1056 GTATAC GTA^TAC
BstEII 547 GGTTACC G^GT[ACGT]ACC
BstPI 547 GGTTACC G^GT[ACGT]ACC
BstUI 41 CGCG CG^CG
BstYI 1011 AGATCT [GA]^GATC[CT]
BstZ17I 1056 GTATAC GTA^TAC
CelII 1402 GCTGAGC GC^T[ACGT]AGC
DrdI 1034 GACATGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 992 TGGCCG [CT]^GGCC[GA]
EcoO65I 547 GGTTACC G^GT[ACGT]ACC
EcoRI 999 GAATTC G^AATTC
EcoT22I 680 ATGCAT ATGCA^T
FauI 42 GCGGG CCCGC(4/6)
Hin1I 256 GACGTC G[GA]^CG[CT]C
HindIII 1313 AAGCTT A^AGCTT
HpaI 1099 GTTAAC GTT^AAC
MfeI 1238 CAATTG C^AATTG
MflI 1011 AGATCT [GA]^GATC[CT]
MslI 129 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1238 CAATTG C^AATTG
MvnI 41 CGCG CG^CG
NsiI 680 ATGCAT ATGCA^T
PflFI 835 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 114 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 874 ATTAAT AT^TAAT
Psp1406I 700 AACGTT AA^CGTT
SacI 1295 GAGCTC GAGCT^C
SexAI 543 ACCAGGT A^CC[AT]GGT
SspBI 323 TGTACA T^GTACA
Tth111I 835 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1011 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 845 GTGCTC G[AT]GC[AT]^C
1295 GAGCTC
BanI 124 GGCACC G^G[CT][GA]CC
643 GGTGCC
BanII 44 GGGCCC G[GA]GC[CT]^C
1295 GAGCTC
BbsI 539 GAAGAC GAAGAC(2/6)
1578 GTCTTC
BfaI 347 CTAG C^TAG
455 CTAG
BpuAI 539 GAAGAC GAAGAC(2/6)
1578 GTCTTC
BsgI 340 CTGCAC GTGCAG(16/14)
149 GTGCAG
BsiHKAI 845 GTGCTC G[AT]GC[AT]^C
1295 GAGCTC
BsiYI 77 CCGCCCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1225 CCTGGAGCTGG
BslI 77 CCGCCCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1225 CCTGGAGCTGG
BsrDI 179 GCAATG GCAATG(2/0)
1249 GCAATG
BsrI 489 ACTGG ACTGG(1/-1)
708 ACTGG
BstAPI 90 GCAGGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1465 GCAGCAGATGC
CfoI 40 GCGC GCG^C
74 GCGC
DraII 114 GGGTCCT [GA]G^G[ACGT]CC[CT]
335 AGGGCCT
EcoO109I 114 GGGTCCT [GA]G^G[ACGT]CC[CT]
335 AGGGCCT
HhaI 40 GCGC GCG^C
74 GCGC
HinP1I 40 GCGC G^CGC
74 GCGC
HincII 19 GTCAAC GT[CT]^[GA]AC
1099 GTTAAC
HindII 19 GTCAAC GT[CT]^[GA]AC
1099 GTTAAC
MaeI 347 CTAG C^TAG
455 CTAG
MaeII 257 ACGT A^CGT
701 ACGT
NspI 1035 ACATGC [GA]CATG^[CT]
1407 GCATGT
PstI 88 CTGCAG CTGCA^G
755 CTGCAG
SapI 1292 GAAGAGC GCTCTTC(1/4)
934 GCTCTTC
TaqI 191 TCGA T^CGA
1412 TCGA
TfiI 520 GATTC G^A[AT]TC
907 GATTC
TthHB8I 191 TCGA T^CGA
1412 TCGA
XcmI 26 CCAAGGCTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1538 CCAACATTCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BmyI BsaJI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI