(Circular) (Six-base) MAPSORT of: pFN21AE6469.seq Check: 8931 from: 1 to: 6295 pFN21AE6469 6295 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2147 2147 Size: 6295 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2265 4105 278 Size: 53 83 186 936 729 1840 2468 Fragments arranged by size: 2468 1840 936 729 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 3638 1937 Size: 1125 576 4594 Fragments arranged by size: 4594 1125 576 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 2706 4422 4795 1563 Size: 1143 1716 373 3063 Fragments arranged by size: 3063 1716 1143 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2385 2855 3282 4358 5742 6010 1632 Size: 753 470 427 1076 1384 268 1917 Fragments arranged by size: 1917 1384 1076 753 470 427 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 5194 6171 1203 Size: 3991 977 1327 Fragments arranged by size: 3991 1327 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2207 1184 Size: 1023 5272 AlwNI CAG_nnn'CTG Cuts at: 4038 5610 4038 Size: 1572 4723 ApaI G_GGCC'C Cuts at: 2053 2053 Size: 6295 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2208 2798 3004 3604 3830 3982 6091 1493 Size: 715 590 206 600 226 152 2109 1697 Fragments arranged by size: 2109 1697 715 600 590 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 2880 4849 160 Size: 872 1848 1969 1606 Fragments arranged by size: 1969 1848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2085 3620 1870 Size: 73 142 1535 4545 Fragments arranged by size: 4545 1535 142 73 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2648 3616 5071 618 Size: 325 217 627 342 519 968 1455 1842 Fragments arranged by size: 1842 1455 968 627 519 342 325 217 BanII G_rGCy'C Cuts at: 1391 2053 3304 3614 1391 Size: 662 1251 310 4072 Fragments arranged by size: 4072 1251 662 310 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2551 3576 928 Size: 971 652 1025 3647 Fragments arranged by size: 3647 1025 971 652 BbvCI CC'TCA_GC Cuts at: 2037 2037 Size: 6295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4486 4520 1903 Size: 34 2549 34 3678 Fragments arranged by size: 3678 2549 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BfrBI ATG'CAT Cuts at: 2687 2687 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4906 136 Size: 107 122 71 631 605 3234 1525 Fragments arranged by size: 3234 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3016 6290 3016 Size: 3274 3021 BlpI GC'TnA_GC Cuts at: 3408 3712 3408 Size: 304 5991 Bme1580I G_kGCm'C Cuts at: 1790 2053 2653 4356 5512 1790 Size: 263 600 1703 1156 2573 Fragments arranged by size: 2573 1703 1156 600 263 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4026 4984 442 Size: 416 53 83 399 24 2609 958 1753 Fragments arranged by size: 2609 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2755 2755 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3252 4955 1239 Size: 2013 1703 2579 Fragments arranged by size: 2579 2013 1703 Bpu10I CC'TnA_GC Cuts at: 2037 2253 2037 Size: 216 6079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4420 5285 5583 4420 Size: 865 298 5132 Fragments arranged by size: 5132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4086 493 Size: 711 2882 2702 Fragments arranged by size: 2882 2702 711 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 4102 4484 5119 Size: 53 83 186 936 729 1840 382 635 Cuts at: 5119 275 Size: 1451 Fragments arranged by size: 1840 1451 936 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6070 Fragments arranged by size: 6070 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3110 1186 Size: 1924 4371 BseYI C'CCAG_C Cuts at: 3575 5498 5994 3575 Size: 1923 496 3876 Fragments arranged by size: 3876 1923 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2175 2330 1163 Size: 573 439 155 5128 Fragments arranged by size: 5128 573 439 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2854 3304 4356 4441 5512 2854 Size: 450 1052 85 1071 3637 Fragments arranged by size: 3637 1071 1052 450 85 BsmI GAATG_Cn' Cuts at: 3370 3806 3899 3370 Size: 436 93 5766 Fragments arranged by size: 5766 436 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2653 2854 3304 3614 4356 4441 Size: 399 263 600 201 450 310 742 85 Cuts at: 4441 5512 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 600 450 399 310 263 201 85 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2147 3650 844 Size: 1303 1503 3489 Fragments arranged by size: 3489 1503 1303 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2191 3261 4790 4964 65 Size: 1183 943 1070 1529 174 1396 Fragments arranged by size: 1529 1396 1183 1070 943 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4939 5126 1700 Size: 110 3129 187 2869 Fragments arranged by size: 3129 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2328 96 Size: 2232 4063 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 2101 3476 794 Size: 1307 1375 3613 Fragments arranged by size: 3613 1375 1307 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 2552 1184 Size: 1368 4927 BstYI r'GATC_y Cuts at: 1972 3016 3625 3992 4377 4394 5846 5932 5944 Size: 1044 609 367 385 17 1452 86 12 Cuts at: 5944 6290 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1044 609 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3063 3063 Size: 6295 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4804 Fragments arranged by size: 4804 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 3811 4605 4625 3811 Size: 794 20 5481 Fragments arranged by size: 5481 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3231 3266 529 Size: 35 2667 35 3558 Fragments arranged by size: 3558 2667 35 35 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3045 5302 809 Size: 507 1729 2257 1802 Fragments arranged by size: 2257 1802 1729 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2997 3742 4631 Size: 54 1425 50 275 18 1167 745 889 Cuts at: 4631 6260 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1167 889 745 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2573 2760 2946 3292 4225 1353 Size: 1220 187 186 346 933 3423 Fragments arranged by size: 3423 1220 933 346 187 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3302 3302 Size: 6295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2385 2855 3252 3282 4358 4955 5742 Size: 393 753 470 397 30 1076 597 787 Cuts at: 5742 6010 1239 Size: 268 1524 Fragments arranged by size: 1524 1076 787 753 597 470 397 393 268 30 EcoO109I rG'GnC_Cy Cuts at: 2120 2341 2120 Size: 221 6074 EcoRI G'AATT_C Cuts at: 3004 3604 3982 3004 Size: 600 378 5317 Fragments arranged by size: 5317 600 378 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 5442 1678 Size: 3764 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2859 2891 3571 3603 4936 Size: 32 135 32 1287 32 680 32 1333 Cuts at: 4936 4968 5010 5042 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1333 1287 680 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2026 3106 3639 3885 669 Size: 988 281 88 1080 533 246 3079 Fragments arranged by size: 3079 1080 988 533 281 246 88 HindIII A'AGCT_T Cuts at: 748 3318 748 Size: 2570 3725 HpaI GTT'AAC Cuts at: 3106 3885 3106 Size: 779 5516 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3243 3894 3243 Size: 651 5644 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2243 5409 5593 1305 Size: 938 3166 184 2007 Fragments arranged by size: 3166 2007 938 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4473 Fragments arranged by size: 4473 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 4253 4612 4771 518 Size: 648 972 2115 359 159 2042 Fragments arranged by size: 2115 2042 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3711 4388 5536 5781 1606 Size: 2105 677 1148 245 2120 Fragments arranged by size: 2120 2105 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4804 Fragments arranged by size: 4804 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NsiI A_TGCA'T Cuts at: 2689 2689 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 3044 3416 3653 5198 1132 Size: 97 1815 372 237 1545 2229 Fragments arranged by size: 2229 1815 1545 372 237 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6295 PfoI T'CCnGG_A Cuts at: 3229 3229 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2120 2120 Size: 6295 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 2754 3865 2754 Size: 1111 5184 PspOMI G'GGCC_C Cuts at: 2049 2049 Size: 6295 PstI C_TGCA'G Cuts at: 830 2097 2764 3647 830 Size: 1267 667 883 3478 Fragments arranged by size: 3478 1267 883 667 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 SacI G_AGCT'C Cuts at: 3304 3304 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SapI GCTCTTCn'nnn_ Cuts at: 2946 3292 2946 Size: 346 5949 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SexAI A'CCwGG_T Cuts at: 2548 2548 Size: 6295 SfcI C'TryA_G Cuts at: 826 1045 2093 2760 3024 3643 4778 5459 5650 Size: 219 1048 667 264 619 1135 681 191 Cuts at: 5650 826 Size: 1471 Fragments arranged by size: 1471 1135 1048 681 667 619 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4399 5300 5562 820 Size: 197 926 2456 901 262 1553 Fragments arranged by size: 2456 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3653 1132 Size: 2521 3774 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2626 3348 3734 513 Size: 552 939 27 595 722 386 3074 Fragments arranged by size: 3074 939 722 595 552 386 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4322 4339 4492 4677 5122 325 Size: 1255 2742 17 153 185 445 1498 Fragments arranged by size: 2742 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2188 2328 2815 Size: 274 80 33 51 1443 211 140 487 Cuts at: 2815 4541 6253 96 Size: 1726 1712 138 Fragments arranged by size: 1726 1712 1443 487 274 211 140 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2985 3017 6255 6287 2985 Size: 32 3238 32 2993 Fragments arranged by size: 3238 2993 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2843 1316 Size: 1527 4768 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 3550 2038 Size: 1512 4783 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 4424 4424 Size: 6295 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2263 4103 276 Size: 53 83 186 936 729 1840 2468 Fragments arranged by size: 2468 1840 936 729 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI