(Linear) (Six-base) MAPSORT of: KIEE6469.seq Check: 7258 from: 1 to: 1590 KIEE6469.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 142 1590 Size: 142 1448 AatII G_ACGT'C Cuts at: 0 260 1590 Size: 260 1330 AccI GT'mk_AC Cuts at: 0 1057 1590 Size: 1057 533 AclI AA'CG_TT Cuts at: 0 701 1590 Size: 701 889 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 850 1277 1590 Size: 380 470 427 313 Fragments arranged by size: 470 427 380 313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 202 1590 Size: 202 1388 ApaI G_GGCC'C Cuts at: 0 48 1590 Size: 48 1542 ApoI r'AATT_y Cuts at: 0 203 793 999 1590 Size: 203 590 206 591 Fragments arranged by size: 591 590 206 203 AseI AT'TA_AT Cuts at: 0 875 1590 Size: 875 715 AvaI C'yCGr_G Cuts at: 0 80 1590 Size: 80 1510 BanI G'GyrC_C Cuts at: 0 124 643 1590 Size: 124 519 947 Fragments arranged by size: 947 519 124 BanII G_rGCy'C Cuts at: 0 48 1299 1590 Size: 48 1251 291 Fragments arranged by size: 1251 291 48 BbsI GAAGACnn'nnnn_ Cuts at: 0 546 1571 1590 Size: 546 1025 19 Fragments arranged by size: 1025 546 19 BbvCI CC'TCA_GC Cuts at: 0 32 1590 Size: 32 1558 BfrBI ATG'CAT Cuts at: 0 682 1590 Size: 682 908 BglII A'GATC_T Cuts at: 0 1011 1590 Size: 1011 579 BlpI GC'TnA_GC Cuts at: 0 1403 1590 Size: 1403 187 Bme1580I G_kGCm'C Cuts at: 0 48 648 1590 Size: 48 600 942 Fragments arranged by size: 942 600 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 750 1590 Size: 750 840 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1247 1590 Size: 1247 343 Bpu10I CC'TnA_GC Cuts at: 0 32 248 1590 Size: 32 216 1342 Fragments arranged by size: 1342 216 32 BsaHI Gr'CG_yC Cuts at: 0 257 1590 Size: 257 1333 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1105 1590 Size: 1105 485 BseYI C'CCAG_C Cuts at: 0 1570 1590 Size: 1570 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 325 1590 Size: 170 155 1265 Fragments arranged by size: 1265 170 155 BsiHKAI G_wGCw'C Cuts at: 0 849 1299 1590 Size: 849 450 291 Fragments arranged by size: 849 450 291 BsmI GAATG_Cn' Cuts at: 0 1365 1590 Size: 1365 225 Bsp1286I G_dGCh'C Cuts at: 0 48 648 849 1299 1590 Size: 48 600 201 450 291 Fragments arranged by size: 600 450 291 201 48 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 142 1590 Size: 142 1448 BsrDI GCAATG_nn' Cuts at: 0 186 1256 1590 Size: 186 1070 334 Fragments arranged by size: 1070 334 186 BsrGI T'GTAC_A Cuts at: 0 323 1590 Size: 323 1267 BstAPI GCAn_nnn'nTGC Cuts at: 0 96 1471 1590 Size: 96 1375 119 Fragments arranged by size: 1375 119 96 BstEII G'GTnAC_C Cuts at: 0 547 1590 Size: 547 1043 BstYI r'GATC_y Cuts at: 0 1011 1590 Size: 1011 579 BstZ17I GTA'TAC Cuts at: 0 1058 1590 Size: 1058 532 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1226 1261 1590 Size: 1226 35 329 Fragments arranged by size: 1226 329 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1040 1590 Size: 1040 550 EaeI y'GGCC_r Cuts at: 0 992 1590 Size: 992 598 EarI CTCTTCn'nnn_ Cuts at: 0 568 755 941 1287 1590 Size: 568 187 186 346 303 Fragments arranged by size: 568 346 303 187 186 EcoICRI GAG'CTC Cuts at: 0 1297 1590 Size: 1297 293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 850 1247 1277 1590 Size: 380 470 397 30 313 Fragments arranged by size: 470 397 380 313 30 EcoO109I rG'GnC_Cy Cuts at: 0 115 336 1590 Size: 115 221 1254 Fragments arranged by size: 1254 221 115 EcoRI G'AATT_C Cuts at: 0 999 1590 Size: 999 591 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 854 886 1566 1590 Size: 854 32 680 24 Fragments arranged by size: 854 680 32 24 HincII GTy'rAC Cuts at: 0 21 1101 1590 Size: 21 1080 489 Fragments arranged by size: 1080 489 21 HindIII A'AGCT_T Cuts at: 0 1313 1590 Size: 1313 277 HpaI GTT'AAC Cuts at: 0 1101 1590 Size: 1101 489 MfeI C'AATT_G Cuts at: 0 1238 1590 Size: 1238 352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 238 1590 Size: 238 1352 MslI CAynn'nnrTG Cuts at: 0 133 1590 Size: 133 1457 NsiI A_TGCA'T Cuts at: 0 684 1590 Size: 684 906 NspI r_CATG'y Cuts at: 0 1039 1411 1590 Size: 1039 372 179 Fragments arranged by size: 1039 372 179 PfoI T'CCnGG_A Cuts at: 0 1224 1590 Size: 1224 366 PpuMI rG'GwC_Cy Cuts at: 0 115 1590 Size: 115 1475 PsiI TTA'TAA Cuts at: 0 749 1590 Size: 749 841 PspOMI G'GGCC_C Cuts at: 0 44 1590 Size: 44 1546 PstI C_TGCA'G Cuts at: 0 92 759 1590 Size: 92 667 831 Fragments arranged by size: 831 667 92 SacI G_AGCT'C Cuts at: 0 1299 1590 Size: 1299 291 SapI GCTCTTCn'nnn_ Cuts at: 0 941 1287 1590 Size: 941 346 303 Fragments arranged by size: 941 346 303 SexAI A'CCwGG_T Cuts at: 0 543 1590 Size: 543 1047 SfcI C'TryA_G Cuts at: 0 88 755 1019 1590 Size: 88 667 264 571 Fragments arranged by size: 667 571 264 88 StyI C'CwwG_G Cuts at: 0 26 621 1343 1590 Size: 26 595 722 247 Fragments arranged by size: 722 595 247 26 TatI w'GTAC_w Cuts at: 0 183 323 810 1590 Size: 183 140 487 780 Fragments arranged by size: 780 487 183 140 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 980 1012 1590 Size: 980 32 578 Fragments arranged by size: 980 578 32 Tth111I GACn'n_nGTC Cuts at: 0 838 1590 Size: 838 752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 33 1545 1590 Size: 33 1512 45 Fragments arranged by size: 1512 45 33 ZraI GAC'GTC Cuts at: 0 258 1590 Size: 258 1332 Enzymes that do cut: AarI AatII AccI AclI AcuI AlfI ApaI ApoI AseI AvaI BanI BanII BbsI BbvCI BfrBI BglII BlpI Bme1580I BplI BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstAPI BstEII BstYI BstZ17I CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI MfeI MmeI MslI NsiI NspI PfoI PpuMI PsiI PspOMI PstI SacI SapI SexAI SfcI StyI TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: Acc65I AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstXI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EagI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XhoI XmaI XmnI