################################## # NO CUT ENZYMES ( 202 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseAI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AflII 85 CTTAAG C^TTAAG AluI 48 AGCT AG^CT AlwI 268 GATCC GGATC(4/5) BanII 105 GGGCTC G[GA]GC[CT]^C BcnI 72 CCGGG CC^[GC]GG BfrI 85 CTTAAG C^TTAAG BmyI 105 GGGCTC G[AGT]GC[ACT]^C BsaWI 111 TCCGGT [AT]^CCGG[AT] BseRI 64 GAGGAG GAGGAG(10/8) BsgI 44 GTGCAG GTGCAG(16/14) BsmBI 53 GAGACG CGTCTC(1/5) Bsp1286I 105 GGGCTC G[AGT]GC[ACT]^C BsrDI 218 GCAATG GCAATG(2/0) BstF5I 167 CATCC GGATG(2/0) Cac8I 135 GCAAGC GC[ACGT]^[ACGT]GC DdeI 203 CTCAG C^T[ACGT]AG DpnII 268 GATC ^GATC EcoNI 225 CCTATTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 167 CATCC GGATG(9/13) HgaI 55 GACGC GACGC(5/10) MboI 268 GATC ^GATC MboII 13 TCTTC GAAGA(8/7) MspA1I 47 CAGCTG C[AC]G^C[GT]G NciI 72 CCGGG CC^[GC]GG NdeII 268 GATC ^GATC NlaIV 74 GGGTCC GG[ACGT]^[ACGT]CC PvuII 47 CAGCTG CAG^CTG Sau3AI 268 GATC ^GATC SfcI 250 CTACAG C^T[GA][CT]AG TspRI 276 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 170 CCGC CCGC(-3/-1) 99 GCGG BbvI 46 GCAGC GCAGC(8/12) 117 GCTGC BpmI 176 CTCCAG CTGGAG(16/14) 50 CTGGAG BsiYI 72 CCGGGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 225 CCTATTTAAGG BslI 72 CCGGGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 225 CCTATTTAAGG BsmAI 53 GAGAC GTCTC(1/5) 201 GTCTC BstNI 78 CCAGG CC^[AT]GG 298 CCAGG EcoRII 78 CCAGG ^CC[AT]GG 298 CCAGG Fnu4HI 46 GCAGC GC^[ACGT]GC 117 GCTGC HapII 72 CCGG C^CGG 112 CCGG HpaII 72 CCGG C^CGG 112 CCGG HphI 61 GGTGA GGTGA(8/7) 16 TCACC ItaI 46 GCAGC GC^[ACGT]GC 117 GCTGC MseI 86 TTAA T^TAA 230 TTAA MspI 72 CCGG C^CGG 112 CCGG MvaI 78 CCAGG CC^[AT]GG 298 CCAGG MwoI 49 GCTGGAGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 172 GCACCTCCAGC SmlI 85 CTTAAG C^T[CT][GA]AG 324 CTTGAG Tru9I 86 TTAA T^TAA 230 TTAA TseI 46 GCAGC G^C[AT]GC 117 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AvaII BssKI Cfr13I MnlI Sau96I ScrFI