Restriction map for
pFN21AE3277
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AhdI AocI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII BbeI BbvCI BcgI BfrI
BglII BsaBI BseAI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I RsrII SacI
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 493 GGTACC G^GTACC
AccI 710 GTCTAC GT^[AC][GT]AC
AgeI 674 ACCGGT A^CCGGT
Aor51HI 281 AGCGCT AGC^GCT
ApaI 560 GGGCCC GGGCC^C
Asp718I 493 GGTACC G^GTACC
BamHI 17 GGATCC G^GATCC
BanII 560 GGGCCC G[GA]GC[CT]^C
BbsI 214 GAAGAC GAAGAC(2/6)
BciVI 890 GTATCC GTATCC(6/5)
BclI 89 TGATCA T^GATCA
BlpI 1489 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1489 GCTCAGC GC^T[ACGT]AGC
BpuAI 214 GAAGAC GAAGAC(2/6)
BsaWI 674 ACCGGT [AT]^CCGG[AT]
BsiEI 2047 CGATCG CG[GA][CT]^CG
BsmBI 1054 CGTCTC CGTCTC(1/5)
BspDI 1510 ATCGAT AT^CGAT
BstXI 1301 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1489 GCTCAGC GC^T[ACGT]AGC
ClaI 1510 ATCGAT AT^CGAT
DrdI 2004 GACTTCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 281 AGCGCT AGC^GCT
FbaI 89 TGATCA T^GATCA
HaeII 281 AGCGCT [GA]GCGC^[CT]
KpnI 493 GGTACC GGTAC^C
NaeI 1033 GCCGGC GCC^GGC
NgoMIV 1033 GCCGGC G^CCGGC
NspI 818 GCATGC [GA]CATG^[CT]
PinAI 674 ACCGGT A^CCGGT
PstI 882 CTGCAG CTGCA^G
PvuI 2047 CGATCG CGAT^CG
SapI 127 GAAGAGC GCTCTTC(1/4)
ScaI 194 AGTACT AGT^ACT
SfiI 1344 GGCCTGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 896 CCCGGG CCC^GGG
SphI 818 GCATGC GCATG^C
Tsp509I 289 AATT ^AATT
XmaCI 896 CCCGGG C^CCGGG
XmaI 896 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 1036 GGCGTC G[GA]^CG[CT]C
1052 GGCGTC
Alw44I 52 GTGCAC G^TGCAC
355 GTGCAC
ApaLI 52 GTGCAC G^TGCAC
355 GTGCAC
AspHI 52 GTGCAC G[AT]GC[AT]^C
355 GTGCAC
AvaI 896 CCCGGG C^[CT]CG[GA]G
1734 CTCGGG
AvrII 1080 CCTAGG C^CTAGG
1266 CCTAGG
BalI 587 TGGCCA TGG^CCA
1539 TGGCCA
BbrPI 1183 CACGTG CAC^GTG
1360 CACGTG
BcnI 896 CCCGG CC^[GC]GG
897 CCGGG
BglI 1345 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1826 GCCTTGAGGGC
BlnI 1080 CCTAGG C^CTAGG
1266 CCTAGG
BsaAI 1183 CACGTG [CT]AC^GT[GA]
1360 CACGTG
BsaHI 1036 GGCGTC G[GA]^CG[CT]C
1052 GGCGTC
BsaI 307 GAGACC GGTCTC(1/5)
380 GAGACC
BsiHKAI 52 GTGCAC G[AT]GC[AT]^C
355 GTGCAC
BsoBI 896 CCCGGG C^[CT]CG[GA]G
1734 CTCGGG
BspHI 644 TCATGA T^CATGA
1235 TCATGA
BspMI 1338 GCAGGT ACCTGC(4/8)
1461 GCAGGT
BsrBI 13 GAGCGG CCGCTC(-3/-3)
130 GAGCGG
BsrDI 1256 GCAATG GCAATG(2/0)
1846 GCAATG
EarI 127 GAAGAG CTCTTC(1/4)
546 GAAGAG
HgaI 1037 GCGTC GACGC(5/10)
1053 GCGTC
Hin1I 1036 GGCGTC G[GA]^CG[CT]C
1052 GGCGTC
Ksp632I 127 GAAGAG CTCTTC(1/4)
546 GAAGAG
MluNI 587 TGGCCA TGG^CCA
1539 TGGCCA
MscI 587 TGGCCA TGG^CCA
1539 TGGCCA
NciI 896 CCCGG CC^[GC]GG
897 CCGGG
PmaCI 1183 CACGTG CAC^GTG
1360 CACGTG
PmlI 1183 CACGTG CAC^GTG
1360 CACGTG
PpuMI 158 AGGTCCT [GA]G^G[AT]CC[CT]
900 GGGACCT
RcaI 644 TCATGA T^CATGA
1235 TCATGA
SfcI 882 CTGCAG C^T[GA][CT]AG
1874 CTGTAG
StuI 476 AGGCCT AGG^CCT
1824 AGGCCT
TfiI 102 GATTC G^A[AT]TC
1513 GATTC
XcmI 1005 CCAGGTGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1175 CCATCAAGCACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII