Restriction map for
pFN21AE3277
(Circular) (Six-base) MAPSORT of: pFN21AE3277.seq Check: 8313 from: 1 to: 6781
pFN21AE3277 6781 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3334 3457 3334
Size: 123 6658
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4591 278
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2716 4124 1937
Size: 779 1408 4594
Fragments arranged by size:
4594 1408 779
Acc65I G'GTAC_C
Cuts at: 2498 4102 2498
Size: 1604 5177
AclI AA'CG_TT
Cuts at: 1563 4908 5281 1563
Size: 3345 373 3063
Fragments arranged by size:
3345 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2663 4844 6228 6496 1632
Size: 1031 2181 1384 268 1917
Fragments arranged by size:
2181 1917 1384 1031 268
AfeI AGC'GCT
Cuts at: 2288 2288
Size: 6781
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6584
AflIII A'CryG_T
Cuts at: 1203 5680 6657 1203
Size: 4477 977 1327
Fragments arranged by size:
4477 1327 977
AgeI A'CCGG_T
Cuts at: 2679 5612 2679
Size: 2933 3848
AhdI GACnn_n'nnGTC
Cuts at: 5510 5510
Size: 6781
AleI CACnn'nnGTG
Cuts at: 1166 2492 2807 3002 3311 1166
Size: 1326 315 195 309 4636
Fragments arranged by size:
4636 1326 315 309 195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2993 1184
Size: 1809 4972
AlwNI CAG_nnn'CTG
Cuts at: 2089 2341 3728 4524 6096 2089
Size: 252 1387 796 1572 2774
Fragments arranged by size:
2774 1572 1387 796 252
ApaI G_GGCC'C
Cuts at: 2569 2569
Size: 6781
ApaLI G'TGCA_C
Cuts at: 2057 2360 4838 5994 2057
Size: 303 2478 1156 2844
Fragments arranged by size:
2844 2478 1156 303
ApoI r'AATT_y
Cuts at: 1493 4090 4316 4468 6577 1493
Size: 2597 226 152 2109 1697
Fragments arranged by size:
2597 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5335 160
Size: 872 4303 1606
Fragments arranged by size:
4303 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
AvaI C'yCGr_G
Cuts at: 1870 1943 2901 3739 4106 1870
Size: 73 958 838 367 4545
Fragments arranged by size:
4545 958 838 367 73
AvrII C'CTAG_G
Cuts at: 3085 3271 3085
Size: 186 6595
BamHI G'GATC_C
Cuts at: 2022 4111 4478 2022
Size: 2089 367 4325
Fragments arranged by size:
4325 2089 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2498 2657 2969 3267
Size: 325 217 627 255 456 159 312 298
Cuts at: 3267 3437 3469 3785 3811 4102 5557 618
Size: 170 32 316 26 291 1455 1842
Fragments arranged by size:
1842 1455 627 456 325 316 312 298
291 255 217 170 159 32 26
BanII G_rGCy'C
Cuts at: 1391 2569 4100 1391
Size: 1178 1531 4072
Fragments arranged by size:
4072 1531 1178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 928
Size: 971 327 5483
Fragments arranged by size:
5483 971 327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4972 5006 1903
Size: 34 3035 34 3678
Fragments arranged by size:
3678 3035 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2906 4673 5883 2906
Size: 1767 1210 3804
Fragments arranged by size:
3804 1767 1210
BclI T'GATC_A
Cuts at: 1548 2094 1548
Size: 546 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3356 3837 5392
Size: 107 122 71 631 605 1684 481 1555
Cuts at: 5392 136
Size: 1525
Fragments arranged by size:
1684 1555 1525 631 605 481 122 107
71
BglII A'GATC_T
Cuts at: 6776 6776
Size: 6781
BlpI GC'TnA_GC
Cuts at: 3495 4198 3495
Size: 703 6078
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2364 2569 2788 2972 3474 3713 3788
Size: 271 303 205 219 184 502 239 75
Cuts at: 3788 3814 4842 5998 1790
Size: 26 1028 1156 2573
Fragments arranged by size:
2573 1156 1028 502 303 271 239 219
205 184 75 26
BmgBI CAC'GTC
Cuts at: 1318 2809 1318
Size: 1491 5290
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3133 3437 4512
Size: 416 53 83 399 24 1716 304 1075
Cuts at: 4512 5470 442
Size: 958 1753
Fragments arranged by size:
1753 1716 1075 958 416 399 304 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3122
Size: 6781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2747 3179 5441 1239
Size: 1085 423 432 2262 2579
Fragments arranged by size:
2579 2262 1085 432 423
Bpu10I CC'TnA_GC
Cuts at: 2465 2520 2691 3647 2465
Size: 55 171 956 5599
Fragments arranged by size:
5599 956 171 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2375 2585 3854 4906 5771 6069 2153
Size: 222 210 1269 1052 865 298 2865
Fragments arranged by size:
2865 1269 1052 865 298 222 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2306 2379 5444 882
Size: 617 807 73 3065 2219
Fragments arranged by size:
3065 2219 807 617 73
BsaAI yAC'GTr
Cuts at: 493 1204 3190 3367 4572 493
Size: 711 1986 177 1205 2702
Fragments arranged by size:
2702 1986 1205 711 177
BsaBI GATnn'nnATC
Cuts at: 6775 6775
Size: 6781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3042 3058 4588 4970
Size: 53 83 186 936 1509 16 1530 382
Cuts at: 4970 5605 275
Size: 635 1451
Fragments arranged by size:
1530 1509 1451 936 635 382 186 83
53 16
BsaWI w'CCGG_w
Cuts at: 2679 5214 5612 5886 6033 6444 2679
Size: 2535 398 274 147 411 3016
Fragments arranged by size:
3016 2535 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3152 3182 3584 3614 1178
Size: 30 165 30 1749 30 402 30 4345
Fragments arranged by size:
4345 1749 402 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 2324 2780 3626 3653 1186
Size: 1135 3 456 846 27 4314
Fragments arranged by size:
4314 1135 846 456 27 3
BseYI C'CCAG_C
Cuts at: 2736 2852 2965 3020 3325 3462 3583 5984 6480
Size: 116 113 55 305 137 121 2401 496
Cuts at: 6480 2736
Size: 3037
Fragments arranged by size:
3037 2401 496 305 137 121 116 113
55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2354 2363 3111 3362 3654 3998
Size: 573 570 48 9 748 251 292 344
Cuts at: 3998 1163
Size: 3946
Fragments arranged by size:
3946 748 573 570 344 292 251 48
9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4055 4231 4992 6020 1540
Size: 275 186 2054 176 761 1028 2301
Fragments arranged by size:
2301 2054 1028 761 275 186 176
BsiHKAI G_wGCw'C
Cuts at: 2061 2364 4842 4927 5998 2061
Size: 303 2478 85 1071 2844
Fragments arranged by size:
2844 2478 1071 303 85
BsmI GAATG_Cn'
Cuts at: 4292 4385 4292
Size: 93 6688
BsmBI CGTCTCn'nnnn_
Cuts at: 3065 3065
Size: 6781
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2364 2569 2788 2972 3474 3713
Size: 399 271 303 205 219 184 502 239
Cuts at: 3713 3788 3814 4100 4842 4927 5998 1391
Size: 75 26 286 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 303 286 271
239 219 205 184 85 75 26
BspEI T'CCGG_A
Cuts at: 6444 6444
Size: 6781
BspHI T'CATG_A
Cuts at: 2649 3240 4670 6400 2649
Size: 591 1430 1730 3030
Fragments arranged by size:
3030 1730 1430 591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3334 3457 4136 844
Size: 2490 123 679 3489
Fragments arranged by size:
3489 2490 679 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 2137 4668 1394
Size: 429 197 117 2531 3507
Fragments arranged by size:
3507 2531 429 197 117
BsrDI GCAATG_nn'
Cuts at: 65 1248 3268 3858 5276 5450 65
Size: 1183 2020 590 1418 174 1396
Fragments arranged by size:
2020 1418 1396 1183 590 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2679 3038 4069 5425 5612 1700
Size: 110 869 359 1031 1356 187 2869
Fragments arranged by size:
2869 1356 1031 869 359 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6781
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6781
BssSI C'ACGA_G
Cuts at: 4841 5853 4841
Size: 1012 5769
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2341 2359 3267 794
Size: 1547 18 908 4308
Fragments arranged by size:
4308 1547 908 18
BstBI TT'CG_AA
Cuts at: 5585 5585
Size: 6781
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6781
BstXI CCAn_nnnn'nTGG
Cuts at: 3313 3313
Size: 6781
BstYI r'GATC_y
Cuts at: 1972 2022 2337 2460 4111 4478 4863 4880 6332
Size: 50 315 123 1651 367 385 17 1452
Cuts at: 6332 6418 6430 6776 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1651 1452 385 367 346 315 123
86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2241 2844 3277 3409 4027 513
Size: 552 939 237 603 433 132 618 3267
Fragments arranged by size:
3267 939 618 603 552 433 237 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6042
Fragments arranged by size:
6042 683 56
BtsI GCAGTG_nn'
Cuts at: 2348 2821 3126 3210 3711 4297 5091 5111 2348
Size: 473 305 84 501 586 794 20 4018
Fragments arranged by size:
4018 794 586 501 473 305 84 20
ClaI AT'CG_AT
Cuts at: 1554 3516 1554
Size: 1962 4819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6746
DraI TTT'AAA
Cuts at: 4085 4085
Size: 6781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4015 5788 809
Size: 507 2699 1773 1802
Fragments arranged by size:
2699 1802 1773 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2592 2741 3544
Size: 54 1425 50 275 18 762 149 803
Cuts at: 3544 3971 4228 5117 6746 8
Size: 427 257 889 1629 43
Fragments arranged by size:
1629 1425 889 803 762 427 275 257
149 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4228 1537
Size: 275 2416 4090
Fragments arranged by size:
4090 2416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2546 4711 1353
Size: 774 419 2165 3423
Fragments arranged by size:
3423 2165 774 419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3111 5368 5742 5888 387
Size: 1064 138 1522 2257 374 146 1280
Fragments arranged by size:
2257 1522 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2663 2747 3179 4844 5441 6228
Size: 393 692 339 84 432 1665 597 787
Cuts at: 6228 6496 1239
Size: 268 1524
Fragments arranged by size:
1665 1524 787 692 597 432 393 339
268 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2303 3071 3608 3680 2072
Size: 231 768 537 72 5173
Fragments arranged by size:
5173 768 537 231 72
EcoO109I rG'GnC_Cy
Cuts at: 2164 2566 2906 3076 3357 3643 2164
Size: 402 340 170 281 286 5302
Fragments arranged by size:
5302 402 340 286 281 170
EcoRI G'AATT_C
Cuts at: 4090 4468 4090
Size: 378 6403
FspI TGC'GCA
Cuts at: 5287 5287
Size: 6781
HaeII r_GCGC'y
Cuts at: 1678 2290 5928 1678
Size: 612 3638 2531
Fragments arranged by size:
3638 2531 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3152 3184 3222 3254 3584
Size: 32 135 32 1580 32 38 32 330
Cuts at: 3584 3616 5422 5454 5496 5528 1373
Size: 32 1806 32 42 32 2626
Fragments arranged by size:
2626 1806 1580 330 135 42 38 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4125 4371 669
Size: 988 281 2187 246 3079
Fragments arranged by size:
3079 2187 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6781
HpaI GTT'AAC
Cuts at: 4371 4371
Size: 6781
KpnI G_GTAC'C
Cuts at: 2502 4106 2502
Size: 1604 5177
MfeI C'AATT_G
Cuts at: 4380 4380
Size: 6781
MluI A'CGCG_T
Cuts at: 6657 6657
Size: 6781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3963 5895 6079 1305
Size: 2658 1932 184 2007
Fragments arranged by size:
2658 2007 1932 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2594 3546 10
Size: 54 1425 343 762 952 3245
Fragments arranged by size:
3245 1425 952 762 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2492 2807 3002 3239 3311 3845
Size: 648 1021 305 315 195 237 72 534
Cuts at: 3845 4739 5098 5257 518
Size: 894 359 159 2042
Fragments arranged by size:
2042 1021 894 648 534 359 315 305
237 195 159 72
MspA1I CmG'CkG
Cuts at: 1606 2704 2740 2886 3020 3255 3420 3456 3725
Size: 1098 36 146 134 235 165 36 269
Cuts at: 3725 4197 4874 6022 6267 1606
Size: 472 677 1148 245 2120
Fragments arranged by size:
2120 1148 1098 677 472 269 245 235
165 146 134 36 36
NaeI GCC'GGC
Cuts at: 3040 3040
Size: 6781
NcoI C'CATG_G
Cuts at: 513 1065 2004 2241 3277 4027 513
Size: 552 939 237 1036 750 3267
Fragments arranged by size:
3267 1036 939 750 552 237
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6781
NgoMIV G'CCGG_C
Cuts at: 3038 3038
Size: 6781
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6781
NotI GC'GGCC_GC
Cuts at: 4228 4228
Size: 6781
NspI r_CATG'y
Cuts at: 1132 1229 2827 4139 5684 1132
Size: 97 1598 1312 1545 2229
Fragments arranged by size:
2229 1598 1545 1312 97
PasI CC'CwG_GG
Cuts at: 1348 2569 3604 1348
Size: 1221 1035 4525
Fragments arranged by size:
4525 1221 1035
PciI A'CATG_T
Cuts at: 5680 5680
Size: 6781
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 2761 3634 1108
Size: 981 672 873 4255
Fragments arranged by size:
4255 981 873 672
PfoI T'CCnGG_A
Cuts at: 2214 3156 2214
Size: 942 5839
PmeI GTTT'AAAC
Cuts at: 4085 4085
Size: 6781
PmlI CAC'GTG
Cuts at: 3190 3367 3190
Size: 177 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 6363 6395 4850
Size: 32 1481 32 5236
Fragments arranged by size:
5236 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2906 2164
Size: 742 6039
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6781
PsiI TTA'TAA
Cuts at: 4351 4351
Size: 6781
PspOMI G'GGCC_C
Cuts at: 2565 2565
Size: 6781
PstI C_TGCA'G
Cuts at: 830 2891 4133 830
Size: 2061 1242 3478
Fragments arranged by size:
3478 2061 1242
PvuI CG_AT'CG
Cuts at: 2001 4055 2001
Size: 2054 4727
PvuII CAG'CTG
Cuts at: 2704 3020 3456 3725 2704
Size: 316 436 269 5760
Fragments arranged by size:
5760 436 316 269
SalI G'TCGA_C
Cuts at: 1936 4123 1936
Size: 2187 4594
SapI GCTCTTCn'nnn_
Cuts at: 2127 2127
Size: 6781
SbfI CC_TGCA'GG
Cuts at: 4133 4133
Size: 6781
ScaI AGT'ACT
Cuts at: 2201 5029 6741 2201
Size: 2828 1712 2241
Fragments arranged by size:
2828 2241 1712
SfcI C'TryA_G
Cuts at: 826 1045 2887 3879 4129 5264 5945 6136 826
Size: 219 1842 992 250 1135 681 191 1471
Fragments arranged by size:
1842 1471 1135 992 681 250 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3356 3356
Size: 6781
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6781
SmaI CCC'GGG
Cuts at: 1872 2903 4108 1872
Size: 1031 1205 4545
Fragments arranged by size:
4545 1205 1031
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 2390 2600 3833 4885 5786
Size: 197 926 225 222 210 1233 1052 901
Cuts at: 5786 6048 820
Size: 262 1553
Fragments arranged by size:
1553 1233 1052 926 901 262 225 222
210 197
SnaBI TAC'GTA
Cuts at: 493 4572 493
Size: 4079 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6781
SphI G_CATG'C
Cuts at: 1132 2827 4139 1132
Size: 1695 1312 3774
Fragments arranged by size:
3774 1695 1312
SspI AAT'ATT
Cuts at: 5 52 4705 5
Size: 47 4653 2081
Fragments arranged by size:
4653 2081 47
StuI AGG'CCT
Cuts at: 1517 2483 3831 1517
Size: 966 1348 4467
Fragments arranged by size:
4467 1348 966
StyI C'CwwG_G
Cuts at: 513 1065 2004 2127 2241 3085 3271 3277 3826
Size: 552 939 123 114 844 186 6 549
Cuts at: 3826 4027 4220 513
Size: 201 193 3074
Fragments arranged by size:
3074 939 844 552 549 201 193 186
123 114 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2381 3255 4045 4808 4825 4978 5163
Size: 1255 801 874 790 763 17 153 185
Cuts at: 5163 5608 325
Size: 445 1498
Fragments arranged by size:
1498 1255 874 801 790 763 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 3906 5027
Size: 274 80 33 51 1443 222 1707 1121
Cuts at: 5027 6739 96
Size: 1712 138
Fragments arranged by size:
1712 1707 1443 1121 274 222 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2863 2895 6741 6773 2863
Size: 32 3846 32 2871
Fragments arranged by size:
3846 2871 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6781
XbaI T'CTAG_A
Cuts at: 4117 4117
Size: 6781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3017 3187 3017
Size: 170 6611
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6781
XmaI C'CCGG_G
Cuts at: 1870 2901 4106 1870
Size: 1031 1205 4545
Fragments arranged by size:
4545 1205 1031
XmnI GAAnn'nnTTC
Cuts at: 4910 4910
Size: 6781
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4589 276
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfoI SrfI SwaI