(Linear) (Six-base) MAPSORT of: KIEE3277.seq Check: 8936 from: 1 to: 2076 KIEE3277.orf With 182 enzymes: SgfI * July 3, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1329 1452 2076 Size: 1329 123 624 Fragments arranged by size: 1329 624 123 AccI GT'mk_AC Cuts at: 0 711 2076 Size: 711 1365 Acc65I G'GTAC_C Cuts at: 0 493 2076 Size: 493 1583 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 2076 Size: 658 1418 AfeI AGC'GCT Cuts at: 0 283 2076 Size: 283 1793 AgeI A'CCGG_T Cuts at: 0 674 2076 Size: 674 1402 AleI CACnn'nnGTG Cuts at: 0 487 802 997 1306 2076 Size: 487 315 195 309 770 Fragments arranged by size: 770 487 315 309 195 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 988 2076 Size: 988 1088 AlwNI CAG_nnn'CTG Cuts at: 0 84 336 1723 2076 Size: 84 252 1387 353 Fragments arranged by size: 1387 353 252 84 ApaI G_GGCC'C Cuts at: 0 564 2076 Size: 564 1512 ApaLI G'TGCA_C Cuts at: 0 52 355 2076 Size: 52 303 1721 Fragments arranged by size: 1721 303 52 AvaI C'yCGr_G Cuts at: 0 896 1734 2076 Size: 896 838 342 Fragments arranged by size: 896 838 342 AvrII C'CTAG_G Cuts at: 0 1080 1266 2076 Size: 1080 186 810 Fragments arranged by size: 1080 810 186 BamHI G'GATC_C Cuts at: 0 17 2076 Size: 17 2059 BanI G'GyrC_C Cuts at: 0 37 493 652 964 1262 1432 1464 1780 Size: 37 456 159 312 298 170 32 316 Cuts at: 1780 1806 2076 Size: 26 270 Fragments arranged by size: 456 316 312 298 270 170 159 37 32 26 BanII G_rGCy'C Cuts at: 0 564 2076 Size: 564 1512 BbsI GAAGACnn'nnnn_ Cuts at: 0 221 2076 Size: 221 1855 BciVI GTATCCnnnnn_n' Cuts at: 0 901 2076 Size: 901 1175 BclI T'GATC_A Cuts at: 0 89 2076 Size: 89 1987 BglI GCCn_nnn'nGGC Cuts at: 0 1351 1832 2076 Size: 1351 481 244 Fragments arranged by size: 1351 481 244 BlpI GC'TnA_GC Cuts at: 0 1490 2076 Size: 1490 586 Bme1580I G_kGCm'C Cuts at: 0 56 359 564 783 967 1469 1708 1783 Size: 56 303 205 219 184 502 239 75 Cuts at: 1783 1809 2076 Size: 26 267 Fragments arranged by size: 502 303 267 239 219 205 184 75 56 26 BmgBI CAC'GTC Cuts at: 0 804 2076 Size: 804 1272 BmrI ACTGGGnnnn_n' Cuts at: 0 1128 1432 2076 Size: 1128 304 644 Fragments arranged by size: 1128 644 304 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1117 2076 Size: 1117 959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 742 1174 2076 Size: 319 423 432 902 Fragments arranged by size: 902 432 423 319 Bpu10I CC'TnA_GC Cuts at: 0 460 515 686 1642 2076 Size: 460 55 171 956 434 Fragments arranged by size: 956 460 434 171 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 370 580 1849 2076 Size: 148 222 210 1269 227 Fragments arranged by size: 1269 227 222 210 148 BsaI GGTCTCn'nnnn_ Cuts at: 0 301 374 2076 Size: 301 73 1702 Fragments arranged by size: 1702 301 73 BsaAI yAC'GTr Cuts at: 0 1185 1362 2076 Size: 1185 177 714 Fragments arranged by size: 1185 714 177 BsaHI Gr'CG_yC Cuts at: 0 1037 1053 2076 Size: 1037 16 1023 Fragments arranged by size: 1037 1023 16 BsaWI w'CCGG_w Cuts at: 0 674 2076 Size: 674 1402 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1147 1177 1579 1609 2076 Size: 1147 30 402 30 467 Fragments arranged by size: 1147 467 402 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 316 319 775 1621 1648 2076 Size: 316 3 456 846 27 428 Fragments arranged by size: 846 456 428 316 27 3 BseYI C'CCAG_C Cuts at: 0 731 847 960 1015 1320 1457 1578 2076 Size: 731 116 113 55 305 137 121 498 Fragments arranged by size: 731 498 305 137 121 116 113 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 349 358 1106 1357 1649 1993 2076 Size: 301 48 9 748 251 292 344 83 Fragments arranged by size: 748 344 301 292 251 83 48 9 BsiEI CG_ry'CG Cuts at: 0 2050 2076 Size: 2050 26 BsiHKAI G_wGCw'C Cuts at: 0 56 359 2076 Size: 56 303 1717 Fragments arranged by size: 1717 303 56 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1060 2076 Size: 1060 1016 Bsp1286I G_dGCh'C Cuts at: 0 56 359 564 783 967 1469 1708 1783 Size: 56 303 205 219 184 502 239 75 Cuts at: 1783 1809 2076 Size: 26 267 Fragments arranged by size: 502 303 267 239 219 205 184 75 56 26 BspHI T'CATG_A Cuts at: 0 644 1235 2076 Size: 644 591 841 Fragments arranged by size: 841 644 591 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1329 1452 2076 Size: 1329 123 624 Fragments arranged by size: 1329 624 123 BsrBI CCG'CTC Cuts at: 0 15 132 2076 Size: 15 117 1944 Fragments arranged by size: 1944 117 15 BsrDI GCAATG_nn' Cuts at: 0 1263 1853 2076 Size: 1263 590 223 Fragments arranged by size: 1263 590 223 BsrFI r'CCGG_y Cuts at: 0 674 1033 2064 2076 Size: 674 359 1031 12 Fragments arranged by size: 1031 674 359 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 336 354 1262 2076 Size: 336 18 908 814 Fragments arranged by size: 908 814 336 18 BstXI CCAn_nnnn'nTGG Cuts at: 0 1308 2076 Size: 1308 768 BstYI r'GATC_y Cuts at: 0 17 332 455 2076 Size: 17 315 123 1621 Fragments arranged by size: 1621 315 123 17 BtgI C'CryG_G Cuts at: 0 236 839 1272 1404 2022 2076 Size: 236 603 433 132 618 54 Fragments arranged by size: 618 603 433 236 132 54 BtsI GCAGTG_nn' Cuts at: 0 343 816 1121 1205 1706 2076 Size: 343 473 305 84 501 370 Fragments arranged by size: 501 473 370 343 305 84 ClaI AT'CG_AT Cuts at: 0 1511 2076 Size: 1511 565 DrdI GACnn_nn'nnGTC Cuts at: 0 2010 2076 Size: 2010 66 EaeI y'GGCC_r Cuts at: 0 587 736 1539 1966 2076 Size: 587 149 803 427 110 Fragments arranged by size: 803 587 427 149 110 EarI CTCTTCn'nnn_ Cuts at: 0 122 541 2076 Size: 122 419 1535 Fragments arranged by size: 1535 419 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1106 2076 Size: 1106 970 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 658 742 1174 2076 Size: 319 339 84 432 902 Fragments arranged by size: 902 432 339 319 84 EcoNI CCTnn'n_nnAGG Cuts at: 0 67 298 1066 1603 1675 2076 Size: 67 231 768 537 72 401 Fragments arranged by size: 768 537 401 231 72 67 EcoO109I rG'GnC_Cy Cuts at: 0 159 561 901 1071 1352 1638 2076 Size: 159 402 340 170 281 286 438 Fragments arranged by size: 438 402 340 286 281 170 159 HaeII r_GCGC'y Cuts at: 0 285 2076 Size: 285 1791 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1147 1179 1217 1249 1579 1611 2076 Size: 1147 32 38 32 330 32 465 Fragments arranged by size: 1147 465 330 38 32 32 32 KpnI G_GTAC'C Cuts at: 0 497 2076 Size: 497 1579 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1958 2076 Size: 1958 118 MscI TGG'CCA Cuts at: 0 589 1541 2076 Size: 589 952 535 Fragments arranged by size: 952 589 535 MslI CAynn'nnrTG Cuts at: 0 182 487 802 997 1234 1306 1840 2076 Size: 182 305 315 195 237 72 534 236 Fragments arranged by size: 534 315 305 237 236 195 182 72 MspA1I CmG'CkG Cuts at: 0 699 735 881 1015 1250 1415 1451 1720 Size: 699 36 146 134 235 165 36 269 Cuts at: 1720 2076 Size: 356 Fragments arranged by size: 699 356 269 235 165 146 134 36 36 NaeI GCC'GGC Cuts at: 0 1035 2076 Size: 1035 1041 NcoI C'CATG_G Cuts at: 0 236 1272 2022 2076 Size: 236 1036 750 54 Fragments arranged by size: 1036 750 236 54 NgoMIV G'CCGG_C Cuts at: 0 1033 2076 Size: 1033 1043 NspI r_CATG'y Cuts at: 0 822 2076 Size: 822 1254 PasI CC'CwG_GG Cuts at: 0 564 1599 2076 Size: 564 1035 477 Fragments arranged by size: 1035 564 477 PflMI CCAn_nnn'nTGG Cuts at: 0 84 756 1629 2076 Size: 84 672 873 447 Fragments arranged by size: 873 672 447 84 PfoI T'CCnGG_A Cuts at: 0 209 1151 2076 Size: 209 942 925 Fragments arranged by size: 942 925 209 PmlI CAC'GTG Cuts at: 0 1185 1362 2076 Size: 1185 177 714 Fragments arranged by size: 1185 714 177 PpuMI rG'GwC_Cy Cuts at: 0 159 901 2076 Size: 159 742 1175 Fragments arranged by size: 1175 742 159 PspOMI G'GGCC_C Cuts at: 0 560 2076 Size: 560 1516 PstI C_TGCA'G Cuts at: 0 886 2076 Size: 886 1190 PvuI CG_AT'CG Cuts at: 0 2050 2076 Size: 2050 26 PvuII CAG'CTG Cuts at: 0 699 1015 1451 1720 2076 Size: 699 316 436 269 356 Fragments arranged by size: 699 436 356 316 269 SapI GCTCTTCn'nnn_ Cuts at: 0 122 2076 Size: 122 1954 ScaI AGT'ACT Cuts at: 0 196 2076 Size: 196 1880 SfcI C'TryA_G Cuts at: 0 882 1874 2076 Size: 882 992 202 Fragments arranged by size: 992 882 202 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1351 2076 Size: 1351 725 SmaI CCC'GGG Cuts at: 0 898 2076 Size: 898 1178 SmlI C'TyrA_G Cuts at: 0 163 385 595 1828 2076 Size: 163 222 210 1233 248 Fragments arranged by size: 1233 248 222 210 163 SphI G_CATG'C Cuts at: 0 822 2076 Size: 822 1254 StuI AGG'CCT Cuts at: 0 478 1826 2076 Size: 478 1348 250 Fragments arranged by size: 1348 478 250 StyI C'CwwG_G Cuts at: 0 122 236 1080 1266 1272 1821 2022 2076 Size: 122 114 844 186 6 549 201 54 Fragments arranged by size: 844 549 201 186 122 114 54 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 376 1250 2040 2076 Size: 376 874 790 36 Fragments arranged by size: 874 790 376 36 TatI w'GTAC_w Cuts at: 0 194 1901 2076 Size: 194 1707 175 Fragments arranged by size: 1707 194 175 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 858 890 2076 Size: 858 32 1186 Fragments arranged by size: 1186 858 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1012 1182 2076 Size: 1012 170 894 Fragments arranged by size: 1012 894 170 XmaI C'CCGG_G Cuts at: 0 896 2076 Size: 896 1180 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AgeI AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbsI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI ClaI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuI PvuII SapI ScaI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AclI AflII AflIII AhdI AloI ApoI AscI AseI AsiSI BaeI BbeI BbvCI BcgI BfrBI BglII BmtI BsaBI BsiWI BsmI BspEI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI RsrII SacI SacII SalI SanDI SbfI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI