################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BbvCI BcgI BclI BfaI BfrI BglII BlnI BsaBI BsaHI BsiWI Bsp1407I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I DraI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII SacII SbfI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1577 GTCGAC GT^[AC][GT]AC AccIII 377 TCCGGA T^CCGGA AflIII 239 ACATGT A^C[GA][CT]GT Aor51HI 500 AGCGCT AGC^GCT Asp700I 1165 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 177 TGCGCA TGC^GCA BbsI 1033 GTCTTC GAAGAC(2/6) BciVI 840 GGATAC GTATCC(6/5) BpmI 598 CTCCAG CTGGAG(16/14) BpuAI 1033 GTCTTC GAAGAC(2/6) BsaI 301 GAGACC GGTCTC(1/5) BseAI 377 TCCGGA T^CCGGA BsiEI 833 CGACCG CG[GA][CT]^CG BsmBI 1136 GAGACG CGTCTC(1/5) BspDI 589 ATCGAT AT^CGAT BspEI 377 TCCGGA T^CCGGA BspHI 524 TCATGA T^CATGA BspLU11I 239 ACATGT A^CATGT BspMI 1059 GCAGGT ACCTGC(4/8) BsrBI 714 CCGCTC CCGCTC(-3/-3) BstEII 1398 GGTGACC G^GT[ACGT]ACC BstPI 1398 GGTGACC G^GT[ACGT]ACC BstXI 521 CCATCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 589 ATCGAT AT^CGAT CpoI 1703 CGGTCCG CG^G[AT]CCG DrdI 1677 GACCTACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 500 AGCGCT AGC^GCT EcoNI 1518 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1398 GGTGACC G^GT[ACGT]ACC FspI 177 TGCGCA TGC^GCA HincII 1577 GTCGAC GT[CT]^[GA]AC HindII 1577 GTCGAC GT[CT]^[GA]AC MroI 377 TCCGGA T^CCGGA NaeI 800 GCCGGC GCC^GGC NgoMIV 800 GCCGGC G^CCGGC PflMI 1347 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1061 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 524 TCATGA T^CATGA RsrII 1703 CGGTCCG CG^G[AT]CCG SalI 1577 GTCGAC G^TCGAC SapI 348 GCTCTTC GCTCTTC(1/4) ScaI 1167 AGTACT AGT^ACT SexAI 1115 ACCAGGT A^CC[AT]GGT SfiI 406 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 1085 GCATGC GCATG^C Van91I 1347 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1178 CCATGCTTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1165 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AlwI 1275 GATCC GGATC(4/5) 536 GGATC AspI 1144 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1633 GACAATGTC AvaI 219 CCCGGG C^[CT]CG[GA]G 276 CCCGGG BalI 117 TGGCCA TGG^CCA 485 TGGCCA BanI 272 GGCACC G^G[CT][GA]CC 1069 GGTGCC BbrPI 1267 CACGTG CAC^GTG 1285 CACGTG BglI 7 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 407 GCCGTGAAGGC BlpI 1257 GCTCAGC GC^T[ACGT]AGC 1605 GCTGAGC Bpu1102I 1257 GCTCAGC GC^T[ACGT]AGC 1605 GCTGAGC BsaAI 1267 CACGTG [CT]AC^GT[GA] 1285 CACGTG BsaWI 377 TCCGGA [AT]^CCGG[AT] 533 ACCGGA BsmFI 896 GGGAC GGGAC(10/14) 1041 GTCCC BsmI 3 GAATGC GAATGC(1/-1) 152 GCATTC BsoBI 219 CCCGGG C^[CT]CG[GA]G 276 CCCGGG BsrFI 254 GCCGGT [GA]^CCGG[CT] 800 GCCGGC BstYI 536 GGATCT [GA]^GATC[CT] 1274 AGATCC CelII 1257 GCTCAGC GC^T[ACGT]AGC 1605 GCTGAGC Cfr10I 254 GCCGGT [GA]^CCGG[CT] 800 GCCGGC DraIII 647 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1348 CACCCTGTG EcoT14I 845 CCTTGG C^C[AT][AT]GG 1565 CCAAGG HaeII 500 AGCGCT [GA]GCGC^[CT] 609 GGCGCT MflI 536 GGATCT [GA]^GATC[CT] 1274 AGATCC MluNI 117 TGGCCA TGG^CCA 485 TGGCCA MscI 117 TGGCCA TGG^CCA 485 TGGCCA MseI 311 TTAA T^TAA 1556 TTAA NspI 239 ACATGT [GA]CATG^[CT] 1085 GCATGC PflFI 1144 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1633 GACAATGTC PmaCI 1267 CACGTG CAC^GTG 1285 CACGTG PmlI 1267 CACGTG CAC^GTG 1285 CACGTG PstI 266 CTGCAG CTGCA^G 1421 CTGCAG SacI 34 GAGCTC GAGCT^C 595 GAGCTC SmaI 219 CCCGGG CCC^GGG 276 CCCGGG StuI 368 AGGCCT AGG^CCT 1232 AGGCCT StyI 845 CCTTGG C^C[AT][AT]GG 1565 CCAAGG TfiI 584 GAATC G^A[AT]TC 883 GAATC Tru9I 311 TTAA T^TAA 1556 TTAA TspRI 1404 CAGTG CA[GC]TG[ACGT][ACGT]^ 1504 CAGTG Tth111I 1144 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1633 GACAATGTC XhoII 536 GGATCT [GA]^GATC[CT] 1274 AGATCC XmaCI 219 CCCGGG C^CCGGG 276 CCCGGG XmaI 219 CCCGGG C^CCGGG 276 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I TthHB8I