(Circular) (Six-base) MAPSORT of: pFN21AE2004.seq Check: 3574 from: 1 to: 6442 pFN21AE2004 6442 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3583 3785 1937 Size: 1646 202 4594 Fragments arranged by size: 4594 1646 202 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 3741 4569 4942 1563 Size: 2178 828 373 3063 Fragments arranged by size: 3063 2178 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2341 2810 2937 3674 4505 5889 6157 1632 Size: 709 469 127 737 831 1384 268 1917 Fragments arranged by size: 1917 1384 831 737 709 469 268 127 AfeI AGC'GCT Cuts at: 2507 2507 Size: 6442 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 2244 5341 6318 1203 Size: 1041 3097 977 1327 Fragments arranged by size: 3097 1327 1041 977 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 2072 3572 1166 Size: 906 1500 4036 Fragments arranged by size: 4036 1500 906 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2090 1184 Size: 906 5536 AlwNI CAG_nnn'CTG Cuts at: 3070 3487 3607 4185 5757 3070 Size: 417 120 578 1572 3755 Fragments arranged by size: 3755 1572 578 417 120 ApaI G_GGCC'C Cuts at: 2232 2981 3443 2232 Size: 749 462 5231 Fragments arranged by size: 5231 749 462 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 3751 3977 4129 6238 1493 Size: 2258 226 152 2109 1697 Fragments arranged by size: 2258 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 2224 2281 3767 1870 Size: 73 281 57 1486 4545 Fragments arranged by size: 4545 1486 281 73 57 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2838 2871 2838 Size: 33 6409 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2277 3074 3763 5218 618 Size: 325 217 627 490 797 689 1455 1842 Fragments arranged by size: 1842 1455 797 689 627 490 325 217 BanII G_rGCy'C Cuts at: 1391 2043 2232 2604 2981 3264 3419 3443 3761 Size: 652 189 372 377 283 155 24 318 Cuts at: 3761 1391 Size: 4072 Fragments arranged by size: 4072 652 377 372 318 283 189 155 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3031 928 Size: 971 1132 4339 Fragments arranged by size: 4339 1132 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4633 4667 1903 Size: 34 2696 34 3678 Fragments arranged by size: 3678 2696 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2839 4334 5544 2839 Size: 1495 1210 3737 Fragments arranged by size: 3737 1495 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6442 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 2418 5053 Size: 107 122 71 631 605 346 400 2635 Cuts at: 5053 136 Size: 1525 Fragments arranged by size: 2635 1525 631 605 400 346 122 107 71 BglII A'GATC_T Cuts at: 6437 6437 Size: 6442 BlpI GC'TnA_GC Cuts at: 3263 3611 3859 3263 Size: 348 248 5846 Fragments arranged by size: 5846 348 248 Bme1580I G_kGCm'C Cuts at: 1790 2232 2280 2981 3079 3443 4503 5659 1790 Size: 442 48 701 98 364 1060 1156 2573 Fragments arranged by size: 2573 1156 1060 701 442 364 98 48 BmgBI CAC'GTC Cuts at: 1318 3145 1318 Size: 1827 4615 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4173 5131 442 Size: 416 53 83 399 24 2756 958 1753 Fragments arranged by size: 2756 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3010 3010 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2588 5102 1239 Size: 1349 2514 2579 Fragments arranged by size: 2579 2514 1349 Bpu10I CC'TnA_GC Cuts at: 2390 3674 2390 Size: 1284 5158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2079 2603 3509 4567 5432 5730 2079 Size: 524 906 1058 865 298 2791 Fragments arranged by size: 2791 1058 906 865 524 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2300 5105 882 Size: 617 801 2805 2219 Fragments arranged by size: 2805 2219 801 617 BsaAI yAC'GTr Cuts at: 493 1204 3274 3292 4233 493 Size: 711 2070 18 941 2702 Fragments arranged by size: 2702 2070 941 711 18 BsaBI GATnn'nnATC Cuts at: 6436 6436 Size: 6442 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4249 4631 5266 275 Size: 53 83 186 936 2716 382 635 1451 Fragments arranged by size: 2716 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 2538 4875 5273 5547 5694 6105 2382 Size: 156 2337 398 274 147 411 2719 Fragments arranged by size: 2719 2337 411 398 274 156 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3281 3311 1178 Size: 30 165 30 1878 30 4309 Fragments arranged by size: 4309 1878 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2936 2939 2983 3248 3289 3545 3647 1186 Size: 1750 3 44 265 41 256 102 3981 Fragments arranged by size: 3981 1750 265 256 102 44 41 3 BseYI C'CCAG_C Cuts at: 2339 2349 2572 2898 5645 6141 2339 Size: 10 223 326 2747 496 2640 Fragments arranged by size: 2747 2640 496 326 223 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2050 2063 2750 3296 1163 Size: 573 314 13 687 546 4309 Fragments arranged by size: 4309 687 573 546 314 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2841 3892 4653 5681 1540 Size: 275 186 840 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 840 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2604 2662 2836 3297 4503 4588 5659 2043 Size: 561 58 174 461 1206 85 1071 2826 Fragments arranged by size: 2826 1206 1071 561 461 174 85 58 BsmI GAATG_Cn' Cuts at: 2014 2157 3953 4046 2014 Size: 143 1796 93 4410 Fragments arranged by size: 4410 1796 143 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3135 3135 Size: 6442 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2232 2280 2604 2662 2836 2981 Size: 399 253 189 48 324 58 174 145 Cuts at: 2981 3079 3264 3297 3419 3443 3761 4503 4588 Size: 98 185 33 122 24 318 742 85 Cuts at: 4588 5659 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 318 253 189 185 174 145 122 98 85 58 48 33 24 BspEI T'CCGG_A Cuts at: 2382 6105 2382 Size: 3723 2719 BspHI T'CATG_A Cuts at: 2529 4331 6061 2529 Size: 1802 1730 2910 Fragments arranged by size: 2910 1802 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3055 3797 844 Size: 2211 742 3489 Fragments arranged by size: 3489 2211 742 BsrBI CCG'CTC Cuts at: 1394 1823 2721 4329 1394 Size: 429 898 1608 3507 Fragments arranged by size: 3507 1608 898 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4937 5111 65 Size: 1183 3689 174 1396 Fragments arranged by size: 3689 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2259 2805 5086 5273 1700 Size: 110 449 546 2281 187 2869 Fragments arranged by size: 2869 2281 546 449 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 4502 5514 4502 Size: 1012 5430 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6442 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 3403 1184 Size: 2219 4223 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 6442 BstYI r'GATC_y Cuts at: 1972 2541 3279 3772 4139 4524 4541 5993 6079 Size: 569 738 493 367 385 17 1452 86 Cuts at: 6079 6091 6437 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 738 569 493 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2044 3192 3705 513 Size: 552 979 1148 513 3250 Fragments arranged by size: 3250 1148 979 552 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2715 487 Size: 683 56 1489 4214 Fragments arranged by size: 4214 1489 683 56 BtsI GCAGTG_nn' Cuts at: 3958 4752 4772 3958 Size: 794 20 5628 Fragments arranged by size: 5628 794 20 ClaI AT'CG_AT Cuts at: 1554 2595 1554 Size: 1041 5401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 3746 3746 Size: 6442 DraIII CAC_nnn'GTG Cuts at: 2657 3358 2657 Size: 701 5741 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3688 5449 809 Size: 507 2372 1761 1802 Fragments arranged by size: 2372 1802 1761 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2410 2490 Size: 54 1425 50 275 18 292 288 80 Cuts at: 2490 3889 4778 6407 8 Size: 1399 889 1629 43 Fragments arranged by size: 1629 1425 1399 889 292 288 275 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3889 1537 Size: 275 2077 4090 Fragments arranged by size: 4090 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2139 2360 2877 2913 4372 1353 Size: 713 73 221 517 36 1459 3423 Fragments arranged by size: 3423 1459 713 517 221 73 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2153 2357 5029 5403 5549 387 Size: 1064 138 564 204 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 564 374 204 146 138 EcoICRI GAG'CTC Cuts at: 2041 2602 2041 Size: 561 5881 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2341 2588 2810 2937 3674 4505 5102 Size: 393 709 247 222 127 737 831 597 Cuts at: 5102 5889 6157 1239 Size: 787 268 1524 Fragments arranged by size: 1524 831 787 737 709 597 393 268 247 222 127 EcoNI CCTnn'n_nnAGG Cuts at: 3527 3527 Size: 6442 EcoO109I rG'GnC_Cy Cuts at: 2228 2420 2977 3067 3440 2228 Size: 192 557 90 373 5230 Fragments arranged by size: 5230 557 373 192 90 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 FspI TGC'GCA Cuts at: 2184 4948 2184 Size: 2764 3678 FspAI rTGC'GCAy Cuts at: 2184 2184 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2509 2618 5589 1678 Size: 831 109 2971 2531 Fragments arranged by size: 2971 2531 831 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2155 2187 3362 3394 5083 Size: 32 135 32 583 32 1175 32 1689 Cuts at: 5083 5115 5157 5189 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1689 1175 583 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3584 3786 4032 669 Size: 988 281 1646 202 246 3079 Fragments arranged by size: 3079 1646 988 281 246 202 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2153 2220 2861 5556 5740 1305 Size: 848 67 641 2695 184 2007 Fragments arranged by size: 2695 2007 848 641 184 67 MscI TGG'CCA Cuts at: 10 64 1489 1832 2124 2492 10 Size: 54 1425 343 292 368 3960 Fragments arranged by size: 3960 1425 368 343 292 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2162 2528 2531 3572 4400 4759 Size: 648 906 90 366 3 1041 828 359 Cuts at: 4759 4918 518 Size: 159 2042 Fragments arranged by size: 2042 1041 906 828 648 366 359 159 90 3 MspA1I CmG'CkG Cuts at: 1606 3858 4535 5683 5928 1606 Size: 2252 677 1148 245 2120 Fragments arranged by size: 2252 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2807 2807 Size: 6442 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5890 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NgoMIV G'CCGG_C Cuts at: 2805 2805 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NspI r_CATG'y Cuts at: 1132 1229 2248 3094 3800 5345 1132 Size: 97 1019 846 706 1545 2229 Fragments arranged by size: 2229 1545 1019 846 706 97 PasI CC'CwG_GG Cuts at: 1348 2974 1348 Size: 1626 4816 PciI A'CATG_T Cuts at: 2244 5341 2244 Size: 3097 3345 PflMI CCAn_nnn'nTGG Cuts at: 1108 3358 1108 Size: 2250 4192 PfoI T'CCnGG_A Cuts at: 2823 2823 Size: 6442 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PmlI CAC'GTG Cuts at: 3274 3292 3274 Size: 18 6424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3067 3067 Size: 6442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PspOMI G'GGCC_C Cuts at: 2228 2977 3439 2228 Size: 749 462 5231 Fragments arranged by size: 5231 749 462 PstI C_TGCA'G Cuts at: 830 2275 3430 3794 830 Size: 1445 1155 364 3478 Fragments arranged by size: 3478 1445 1155 364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 RsrII CG'GwC_CG Cuts at: 3709 3709 Size: 6442 SacI G_AGCT'C Cuts at: 2043 2604 2043 Size: 561 5881 SalI G'TCGA_C Cuts at: 1936 3582 3784 1936 Size: 1646 202 4594 Fragments arranged by size: 4594 1646 202 SapI GCTCTTCn'nnn_ Cuts at: 2360 2360 Size: 6442 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 3174 4690 6402 3174 Size: 1516 1712 3214 Fragments arranged by size: 3214 1712 1516 SexAI A'CCwGG_T Cuts at: 3120 3120 Size: 6442 SfcI C'TryA_G Cuts at: 826 1045 2271 3420 3426 3790 4925 5606 5797 Size: 219 1226 1149 6 364 1135 681 191 Cuts at: 5797 826 Size: 1471 Fragments arranged by size: 1471 1226 1149 1135 681 364 219 191 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2418 2418 Size: 6442 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 2226 2283 3769 1872 Size: 354 57 1486 4545 Fragments arranged by size: 4545 1486 354 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 2094 2618 3524 4546 5447 5709 Size: 197 926 151 524 906 1022 901 262 Cuts at: 5709 820 Size: 1553 Fragments arranged by size: 1553 1022 926 906 901 524 262 197 151 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 3094 3800 1132 Size: 1962 706 3774 Fragments arranged by size: 3774 1962 706 SspI AAT'ATT Cuts at: 5 52 4366 5 Size: 47 4314 2081 Fragments arranged by size: 4314 2081 47 StuI AGG'CCT Cuts at: 1517 2375 3239 1517 Size: 858 864 4720 Fragments arranged by size: 4720 864 858 StyI C'CwwG_G Cuts at: 513 1065 2850 3570 3881 513 Size: 552 1785 720 311 3074 Fragments arranged by size: 3074 1785 720 552 311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2828 2855 3062 3447 3611 4469 4486 Size: 1255 1248 27 207 385 164 858 17 Cuts at: 4486 4639 4824 5269 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1248 858 445 385 207 185 164 153 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2951 3172 4688 Size: 274 80 33 51 1443 974 221 1516 Cuts at: 4688 6400 96 Size: 1712 138 Fragments arranged by size: 1712 1516 1443 974 274 221 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3281 3313 3686 3718 6402 6434 3281 Size: 32 373 32 2684 32 3289 Fragments arranged by size: 3289 2684 373 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3152 3641 1316 Size: 1836 489 4117 Fragments arranged by size: 4117 1836 489 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3190 3190 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 2224 2281 3767 1870 Size: 354 57 1486 4545 Fragments arranged by size: 4545 1486 354 57 XmnI GAAnn'nnTTC Cuts at: 3174 4571 3174 Size: 1397 5045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FseI KasI NarI NruI NsiI PacI PspXI PsrI PvuII SacII SanDI SfoI SrfI SwaI