(Linear) (Six-base) MAPSORT of: KIEE2004.seq Check: 8424 from: 1 to: 1737 KIEE2004.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AccI GT'mk_AC Cuts at: 0 1578 1737 Size: 1578 159 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 805 932 1669 1737 Size: 336 469 127 737 68 Fragments arranged by size: 737 469 336 127 68 AfeI AGC'GCT Cuts at: 0 502 1737 Size: 502 1235 AflIII A'CryG_T Cuts at: 0 239 1737 Size: 239 1498 AleI CACnn'nnGTG Cuts at: 0 67 1567 1737 Size: 67 1500 170 Fragments arranged by size: 1500 170 67 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 85 1737 Size: 85 1652 AlwNI CAG_nnn'CTG Cuts at: 0 1065 1482 1602 1737 Size: 1065 417 120 135 Fragments arranged by size: 1065 417 135 120 ApaI G_GGCC'C Cuts at: 0 227 976 1438 1737 Size: 227 749 462 299 Fragments arranged by size: 749 462 299 227 AvaI C'yCGr_G Cuts at: 0 219 276 1737 Size: 219 57 1461 Fragments arranged by size: 1461 219 57 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 833 866 1737 Size: 833 33 871 Fragments arranged by size: 871 833 33 BanI G'GyrC_C Cuts at: 0 272 1069 1737 Size: 272 797 668 Fragments arranged by size: 797 668 272 BanII G_rGCy'C Cuts at: 0 38 227 599 976 1259 1414 1438 1737 Size: 38 189 372 377 283 155 24 299 Fragments arranged by size: 377 372 299 283 189 155 38 24 BbsI GAAGACnn'nnnn_ Cuts at: 0 1026 1737 Size: 1026 711 BciVI GTATCCnnnnn_n' Cuts at: 0 834 1737 Size: 834 903 BglI GCCn_nnn'nGGC Cuts at: 0 13 413 1737 Size: 13 400 1324 Fragments arranged by size: 1324 400 13 BlpI GC'TnA_GC Cuts at: 0 1258 1606 1737 Size: 1258 348 131 Fragments arranged by size: 1258 348 131 Bme1580I G_kGCm'C Cuts at: 0 227 275 976 1074 1438 1737 Size: 227 48 701 98 364 299 Fragments arranged by size: 701 364 299 227 98 48 BmgBI CAC'GTC Cuts at: 0 1140 1737 Size: 1140 597 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1005 1737 Size: 1005 732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 1737 Size: 583 1154 Bpu10I CC'TnA_GC Cuts at: 0 385 1669 1737 Size: 385 1284 68 Fragments arranged by size: 1284 385 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 598 1504 1737 Size: 74 524 906 233 Fragments arranged by size: 906 524 233 74 BsaI GGTCTCn'nnnn_ Cuts at: 0 295 1737 Size: 295 1442 BsaAI yAC'GTr Cuts at: 0 1269 1287 1737 Size: 1269 18 450 Fragments arranged by size: 1269 450 18 BsaWI w'CCGG_w Cuts at: 0 377 533 1737 Size: 377 156 1204 Fragments arranged by size: 1204 377 156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1276 1306 1737 Size: 1276 30 431 Fragments arranged by size: 1276 431 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 931 934 978 1243 1284 1540 1642 1737 Size: 931 3 44 265 41 256 102 95 Fragments arranged by size: 931 265 256 102 95 44 41 3 BseYI C'CCAG_C Cuts at: 0 334 344 567 893 1737 Size: 334 10 223 326 844 Fragments arranged by size: 844 334 326 223 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 58 745 1291 1737 Size: 45 13 687 546 446 Fragments arranged by size: 687 546 446 45 13 BsiEI CG_ry'CG Cuts at: 0 836 1737 Size: 836 901 BsiHKAI G_wGCw'C Cuts at: 0 38 599 657 831 1292 1737 Size: 38 561 58 174 461 445 Fragments arranged by size: 561 461 445 174 58 38 BsmI GAATG_Cn' Cuts at: 0 9 152 1737 Size: 9 143 1585 Fragments arranged by size: 1585 143 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1130 1737 Size: 1130 607 Bsp1286I G_dGCh'C Cuts at: 0 38 227 275 599 657 831 976 1074 Size: 38 189 48 324 58 174 145 98 Cuts at: 1074 1259 1292 1414 1438 1737 Size: 185 33 122 24 299 Fragments arranged by size: 324 299 189 185 174 145 122 98 58 48 38 33 24 BspEI T'CCGG_A Cuts at: 0 377 1737 Size: 377 1360 BspHI T'CATG_A Cuts at: 0 524 1737 Size: 524 1213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1050 1737 Size: 1050 687 BsrBI CCG'CTC Cuts at: 0 716 1737 Size: 716 1021 BsrFI r'CCGG_y Cuts at: 0 254 800 1737 Size: 254 546 937 Fragments arranged by size: 937 546 254 BstEII G'GTnAC_C Cuts at: 0 1398 1737 Size: 1398 339 BstXI CCAn_nnnn'nTGG Cuts at: 0 528 1737 Size: 528 1209 BstYI r'GATC_y Cuts at: 0 536 1274 1737 Size: 536 738 463 Fragments arranged by size: 738 536 463 BtgI C'CryG_G Cuts at: 0 39 1187 1700 1737 Size: 39 1148 513 37 Fragments arranged by size: 1148 513 39 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 710 1737 Size: 710 1027 ClaI AT'CG_AT Cuts at: 0 590 1737 Size: 590 1147 DraIII CAC_nnn'GTG Cuts at: 0 652 1353 1737 Size: 652 701 384 Fragments arranged by size: 701 652 384 DrdI GACnn_nn'nnGTC Cuts at: 0 1683 1737 Size: 1683 54 EaeI y'GGCC_r Cuts at: 0 117 405 485 1737 Size: 117 288 80 1252 Fragments arranged by size: 1252 288 117 80 EarI CTCTTCn'nnn_ Cuts at: 0 61 134 355 872 908 1737 Size: 61 73 221 517 36 829 Fragments arranged by size: 829 517 221 73 61 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 148 352 1737 Size: 148 204 1385 Fragments arranged by size: 1385 204 148 EcoICRI GAG'CTC Cuts at: 0 36 597 1737 Size: 36 561 1140 Fragments arranged by size: 1140 561 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 583 805 932 1669 1737 Size: 336 247 222 127 737 68 Fragments arranged by size: 737 336 247 222 127 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 1522 1737 Size: 1522 215 EcoO109I rG'GnC_Cy Cuts at: 0 223 415 972 1062 1435 1737 Size: 223 192 557 90 373 302 Fragments arranged by size: 557 373 302 223 192 90 FspI TGC'GCA Cuts at: 0 179 1737 Size: 179 1558 FspAI rTGC'GCAy Cuts at: 0 179 1737 Size: 179 1558 HaeII r_GCGC'y Cuts at: 0 504 613 1737 Size: 504 109 1124 Fragments arranged by size: 1124 504 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 150 182 1357 1389 1737 Size: 150 32 1175 32 348 Fragments arranged by size: 1175 348 150 32 32 HincII GTy'rAC Cuts at: 0 1579 1737 Size: 1579 158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 148 215 856 1737 Size: 148 67 641 881 Fragments arranged by size: 881 641 148 67 MscI TGG'CCA Cuts at: 0 119 487 1737 Size: 119 368 1250 Fragments arranged by size: 1250 368 119 MslI CAynn'nnrTG Cuts at: 0 67 157 523 526 1567 1737 Size: 67 90 366 3 1041 170 Fragments arranged by size: 1041 366 170 90 67 3 NaeI GCC'GGC Cuts at: 0 802 1737 Size: 802 935 NgoMIV G'CCGG_C Cuts at: 0 800 1737 Size: 800 937 NspI r_CATG'y Cuts at: 0 243 1089 1737 Size: 243 846 648 Fragments arranged by size: 846 648 243 PasI CC'CwG_GG Cuts at: 0 969 1737 Size: 969 768 PciI A'CATG_T Cuts at: 0 239 1737 Size: 239 1498 PflMI CCAn_nnn'nTGG Cuts at: 0 1353 1737 Size: 1353 384 PfoI T'CCnGG_A Cuts at: 0 818 1737 Size: 818 919 PmlI CAC'GTG Cuts at: 0 1269 1287 1737 Size: 1269 18 450 Fragments arranged by size: 1269 450 18 PpuMI rG'GwC_Cy Cuts at: 0 1062 1737 Size: 1062 675 PspOMI G'GGCC_C Cuts at: 0 223 972 1434 1737 Size: 223 749 462 303 Fragments arranged by size: 749 462 303 223 PstI C_TGCA'G Cuts at: 0 270 1425 1737 Size: 270 1155 312 Fragments arranged by size: 1155 312 270 RsrII CG'GwC_CG Cuts at: 0 1704 1737 Size: 1704 33 SacI G_AGCT'C Cuts at: 0 38 599 1737 Size: 38 561 1138 Fragments arranged by size: 1138 561 38 SalI G'TCGA_C Cuts at: 0 1577 1737 Size: 1577 160 SapI GCTCTTCn'nnn_ Cuts at: 0 355 1737 Size: 355 1382 ScaI AGT'ACT Cuts at: 0 1169 1737 Size: 1169 568 SexAI A'CCwGG_T Cuts at: 0 1115 1737 Size: 1115 622 SfcI C'TryA_G Cuts at: 0 266 1415 1421 1737 Size: 266 1149 6 316 Fragments arranged by size: 1149 316 266 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 413 1737 Size: 413 1324 SmaI CCC'GGG Cuts at: 0 221 278 1737 Size: 221 57 1459 Fragments arranged by size: 1459 221 57 SmlI C'TyrA_G Cuts at: 0 89 613 1519 1737 Size: 89 524 906 218 Fragments arranged by size: 906 524 218 89 SphI G_CATG'C Cuts at: 0 1089 1737 Size: 1089 648 StuI AGG'CCT Cuts at: 0 370 1234 1737 Size: 370 864 503 Fragments arranged by size: 864 503 370 StyI C'CwwG_G Cuts at: 0 845 1565 1737 Size: 845 720 172 Fragments arranged by size: 845 720 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 823 850 1057 1442 1606 1737 Size: 823 27 207 385 164 131 Fragments arranged by size: 823 385 207 164 131 27 TatI w'GTAC_w Cuts at: 0 946 1167 1737 Size: 946 221 570 Fragments arranged by size: 946 570 221 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1276 1308 1681 1713 1737 Size: 1276 32 373 32 24 Fragments arranged by size: 1276 373 32 32 24 Tth111I GACn'n_nGTC Cuts at: 0 1147 1636 1737 Size: 1147 489 101 Fragments arranged by size: 1147 489 101 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1185 1737 Size: 1185 552 XmaI C'CCGG_G Cuts at: 0 219 276 1737 Size: 219 57 1461 Fragments arranged by size: 1461 219 57 XmnI GAAnn'nnTTC Cuts at: 0 1169 1737 Size: 1169 568 Enzymes that do cut: AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI AvaI BaeI BanI BanII BbsI BciVI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstEII BstXI BstYI BtgI BtgZI ClaI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII MmeI MscI MslI NaeI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI RsrII SacI SalI SapI ScaI SexAI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglII BmrI BmtI BsaBI BsaHI BsiWI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI CspCI DraI EagI EcoRI EcoRV FalI FseI HindIII HpaI KasI KpnI MfeI MluI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII SacII SanDI SbfI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI