Restriction map for
pFN21AE5810
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# NO CUT ENZYMES ( 86 enzymes) #
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AccIII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BbeI BclI
BfrI BglI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
FbaI FseI FspI HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspI SwaI Tth111I Van91I
XbaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 1460 GGTACC G^GTACC
AccI 3454 GTCGAC GT^[AC][GT]AC
Asp718I 1460 GGTACC G^GTACC
BamHI 3239 GGATCC G^GATCC
BbrPI 4249 CACGTG CAC^GTG
BbvCI 1936 GCTGAGG CCTCAGC(-5/-2)
BcgI 603 CGACATGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4113 GGATAC GTATCC(6/5)
BlpI 2109 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2109 GCTCAGC GC^T[ACGT]AGC
BsrBI 1509 CCGCTC CCGCTC(-3/-3)
CelII 2109 GCTCAGC GC^T[ACGT]AGC
EcoT14I 1386 CCATGG C^C[AT][AT]GG
EcoT22I 607 ATGCAT ATGCA^T
HaeII 4297 AGCGCC [GA]GCGC^[CT]
KpnI 1460 GGTACC GGTAC^C
NaeI 3608 GCCGGC GCC^GGC
NcoI 1386 CCATGG C^CATGG
NdeI 1340 CATATG CA^TATG
NgoMIV 3608 GCCGGC G^CCGGC
NsiI 607 ATGCAT ATGCA^T
PmaCI 4249 CACGTG CAC^GTG
PmlI 4249 CACGTG CAC^GTG
SacI 4153 GAGCTC GAGCT^C
SalI 3454 GTCGAC G^TCGAC
SexAI 2421 ACCTGGT A^CC[AT]GGT
SmaI 3581 CCCGGG CCC^GGG
StyI 1386 CCATGG C^C[AT][AT]GG
XcmI 1541 CCAAGACACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3581 CCCGGG C^CCGGG
XmaI 3581 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AatII 685 GACGTC GACGT^C
2169 GACGTC
AcyI 685 GACGTC G[GA]^CG[CT]C
2169 GACGTC
Alw44I 1471 GTGCAC G^TGCAC
2365 GTGCAC
AocI 3637 CCTGAGG CC^T[ACGT]AGG
3936 CCTCAGG
ApaI 1320 GGGCCC GGGCC^C
2316 GGGCCC
ApaLI 1471 GTGCAC G^TGCAC
2365 GTGCAC
Asp700I 948 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2767 GAAGATGTTC
BfaI 269 CTAG C^TAG
356 CTAG
BglII 368 AGATCT A^GATCT
753 AGATCT
BsaHI 685 GACGTC G[GA]^CG[CT]C
2169 GACGTC
Bsp1407I 2509 TGTACA T^GTACA
3230 TGTACA
BspLU11I 2337 ACATGT A^CATGT
4076 ACATGT
BsrFI 3548 GCCGGT [GA]^CCGG[CT]
3608 GCCGGC
BsrGI 2509 TGTACA T^GTACA
3230 TGTACA
Bsu36I 3637 CCTGAGG CC^T[ACGT]AGG
3936 CCTCAGG
Cfr10I 3548 GCCGGT [GA]^CCGG[CT]
3608 GCCGGC
DraIII 397 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2420 CACCTGGTG
DrdI 7 GACTCGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1273 GACAGCATGGTC
Eco81I 3637 CCTGAGG CC^T[ACGT]AGG
3936 CCTCAGG
EcoRV 2094 GATATC GAT^ATC
3897 GATATC
Hin1I 685 GACGTC G[GA]^CG[CT]C
2169 GACGTC
MaeI 269 CTAG C^TAG
356 CTAG
PaeR7I 1769 CTCGAG C^TCGAG
3874 CTCGAG
SbfI 1464 CCTGCAGG CCTGCA^GG
2928 CCTGCAGG
SphI 609 GCATGC GCATG^C
678 GCATGC
Sse8387I 1464 CCTGCAGG CCTGCA^GG
2928 CCTGCAGG
SspBI 2509 TGTACA T^GTACA
3230 TGTACA
XhoI 1769 CTCGAG C^TCGAG
3874 CTCGAG
XmnI 948 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2767 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII