(Circular) (Six-base) MAPSORT of: pFN21AE5810.seq Check: 7628 from: 1 to: 9070 pFN21AE5810 9070 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3463 3463 Size: 9070 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2694 4178 6880 278 Size: 53 83 186 936 1158 1484 2702 2468 Fragments arranged by size: 2702 2468 1484 1158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5460 6413 1937 Size: 3523 953 4594 Fragments arranged by size: 4594 3523 953 Acc65I G'GTAC_C Cuts at: 3465 6391 3465 Size: 2926 6144 AclI AA'CG_TT Cuts at: 1563 7197 7570 1563 Size: 5634 373 3063 Fragments arranged by size: 5634 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2620 2947 2972 3632 3845 3909 4848 4896 Size: 988 327 25 660 213 64 939 48 Cuts at: 4896 5195 5392 7133 8517 8785 1632 Size: 299 197 1741 1384 268 1917 Fragments arranged by size: 1917 1741 1384 988 939 660 327 299 268 213 197 64 48 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8873 AflIII A'CryG_T Cuts at: 1203 3741 4270 4342 6081 6253 7969 8946 1203 Size: 2538 529 72 1739 172 1716 977 1327 Fragments arranged by size: 2538 1739 1716 1327 977 529 172 72 AgeI A'CCGG_T Cuts at: 7901 7901 Size: 9070 AhdI GACnn_n'nnGTC Cuts at: 7799 7799 Size: 9070 AleI CACnn'nnGTG Cuts at: 1166 4377 5373 5865 1166 Size: 3211 996 492 4371 Fragments arranged by size: 4371 3211 996 492 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4737 1184 Size: 3553 5517 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4478 4510 4478 Size: 32 9038 AlwNI CAG_nnn'CTG Cuts at: 2173 3220 4192 4850 5179 5687 6813 8385 2173 Size: 1047 972 658 329 508 1126 1572 2858 Fragments arranged by size: 2858 1572 1126 1047 972 658 508 329 ApaI G_GGCC'C Cuts at: 3329 4325 3329 Size: 996 8074 ApaLI G'TGCA_C Cuts at: 3476 4370 7127 8283 3476 Size: 894 2757 1156 4263 Fragments arranged by size: 4263 2757 1156 894 ApoI r'AATT_y Cuts at: 1493 4508 4818 5011 6379 6605 6757 8866 1493 Size: 3015 310 193 1368 226 152 2109 1697 Fragments arranged by size: 3015 2109 1697 1368 310 226 193 152 AseI AT'TA_AT Cuts at: 160 1032 7624 160 Size: 872 6592 1606 Fragments arranged by size: 6592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9070 AvaI C'yCGr_G Cuts at: 1870 1943 2035 3774 4324 5586 5879 6395 1870 Size: 73 92 1739 550 1262 293 516 4545 Fragments arranged by size: 4545 1739 1262 550 516 293 92 73 BamHI G'GATC_C Cuts at: 5244 6400 6767 5244 Size: 1156 367 7547 Fragments arranged by size: 7547 1156 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3465 5375 6231 6391 7846 Size: 325 217 627 1678 1910 856 160 1455 Cuts at: 7846 618 Size: 1842 Fragments arranged by size: 1910 1842 1678 1455 856 627 325 217 160 BanII G_rGCy'C Cuts at: 1391 3329 3633 3951 4325 5579 6162 6389 1391 Size: 1938 304 318 374 1254 583 227 4072 Fragments arranged by size: 4072 1938 1254 583 374 318 304 227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2433 3234 3409 6008 6042 928 Size: 971 534 801 175 2599 34 3956 Fragments arranged by size: 3956 2599 971 801 534 175 34 BbvCI CC'TCA_GC Cuts at: 3942 3942 Size: 9070 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2597 2631 7261 7295 1903 Size: 34 660 34 4630 34 3678 Fragments arranged by size: 4630 3678 660 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6112 6962 8172 6112 Size: 850 1210 7010 Fragments arranged by size: 7010 1210 850 BclI T'GATC_A Cuts at: 1548 1548 Size: 9070 BfrBI ATG'CAT Cuts at: 2614 2614 Size: 9070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7681 136 Size: 107 122 71 631 605 6009 1525 Fragments arranged by size: 6009 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2373 2758 9065 2373 Size: 385 6307 2378 Fragments arranged by size: 6307 2378 385 BlpI GC'TnA_GC Cuts at: 4115 6487 4115 Size: 2372 6698 Bme1580I G_kGCm'C Cuts at: 1790 2125 3329 3480 4325 4339 4374 5380 6236 Size: 335 1204 151 845 14 35 1006 856 Cuts at: 6236 7131 8287 1790 Size: 895 1156 2573 Fragments arranged by size: 2573 1204 1156 1006 895 856 845 335 151 35 14 BmgBI CAC'GTC Cuts at: 1318 4271 1318 Size: 2953 6117 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3241 3692 3983 Size: 416 53 83 399 24 1824 451 291 Cuts at: 3983 4549 6801 7759 442 Size: 566 2252 958 1753 Fragments arranged by size: 2252 1824 1753 958 566 451 416 399 291 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2887 3346 4742 5668 2887 Size: 459 1396 926 6289 Fragments arranged by size: 6289 1396 926 459 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2370 2874 3341 4157 4189 4572 4702 4829 Size: 1131 504 467 816 32 383 130 127 Cuts at: 4829 5108 5156 5555 6123 6176 6296 7730 1239 Size: 279 48 399 568 53 120 1434 2579 Fragments arranged by size: 2579 1434 1131 816 568 504 467 399 383 279 130 127 120 53 48 32 Bpu10I CC'TnA_GC Cuts at: 3942 3942 Size: 9070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 2296 3551 4972 6146 7195 8060 8358 2210 Size: 86 1255 1421 1174 1049 865 298 2922 Fragments arranged by size: 2922 1421 1255 1174 1049 865 298 86 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2387 4678 4923 5436 7733 882 Size: 617 888 2291 245 513 2297 2219 Fragments arranged by size: 2297 2291 2219 888 617 513 245 BsaAI yAC'GTr Cuts at: 493 1204 2563 3742 6256 6861 493 Size: 711 1359 1179 2514 605 2702 Fragments arranged by size: 2702 2514 1359 1179 711 605 BsaBI GATnn'nnATC Cuts at: 9064 9064 Size: 9070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2691 4175 6877 7259 Size: 53 83 186 936 1158 1484 2702 382 Cuts at: 7259 7894 275 Size: 635 1451 Fragments arranged by size: 2702 1484 1451 1158 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7503 7901 8175 8322 8733 7503 Size: 398 274 147 411 7840 Fragments arranged by size: 7840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4260 4290 5232 5262 5348 Size: 30 165 30 2857 30 942 30 86 Cuts at: 5348 5378 1178 Size: 30 4870 Fragments arranged by size: 4870 2857 942 165 86 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2879 3125 3512 3959 4673 5240 5575 5780 Size: 1693 246 387 447 714 567 335 205 Cuts at: 5780 5948 5951 1186 Size: 168 3 4305 Fragments arranged by size: 4305 1693 714 567 447 387 335 246 205 168 3 BseYI C'CCAG_C Cuts at: 2317 3614 4004 4348 5656 5947 6113 8273 8769 Size: 1297 390 344 1308 291 166 2160 496 Cuts at: 8769 2317 Size: 2618 Fragments arranged by size: 2618 2160 1308 1297 496 390 344 291 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2362 3126 3954 4407 4594 5673 5678 Size: 573 626 764 828 453 187 1079 5 Cuts at: 5678 5889 1163 Size: 211 4344 Fragments arranged by size: 4344 1079 828 764 626 573 453 211 187 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6520 7281 8309 1540 Size: 275 186 4519 761 1028 2301 Fragments arranged by size: 4519 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3174 3380 3480 3783 4278 4290 4374 4890 5142 Size: 206 100 303 495 12 84 516 252 Cuts at: 5142 6162 7131 7216 8287 3174 Size: 1020 969 85 1071 3957 Fragments arranged by size: 3957 1071 1020 969 516 495 303 252 206 100 85 84 12 BsmI GAATG_Cn' Cuts at: 2182 2826 3257 6581 6674 2182 Size: 644 431 3324 93 4578 Fragments arranged by size: 4578 3324 644 431 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2125 3174 3329 3380 3480 3633 3783 Size: 399 335 1049 155 51 100 153 150 Cuts at: 3783 3951 4278 4290 4325 4339 4374 4890 5142 Size: 168 327 12 35 14 35 516 252 Cuts at: 5142 5380 5579 6162 6236 6389 7131 7216 8287 Size: 238 199 583 74 153 742 85 1071 Cuts at: 8287 1391 Size: 2174 Fragments arranged by size: 2174 1071 1049 742 583 516 399 335 327 252 238 199 168 155 153 153 150 100 85 74 51 35 35 14 12 BspEI T'CCGG_A Cuts at: 8733 8733 Size: 9070 BspHI T'CATG_A Cuts at: 6959 8689 6959 Size: 1730 7340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2785 3463 3477 4941 5853 6425 844 Size: 1941 678 14 1464 912 572 3489 Fragments arranged by size: 3489 1941 1464 912 678 572 14 BsrBI CCG'CTC Cuts at: 1394 1823 3516 6957 1394 Size: 429 1693 3441 3507 Fragments arranged by size: 3507 3441 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7565 7739 65 Size: 1183 6317 174 1396 Fragments arranged by size: 6317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5553 5613 7714 7901 1700 Size: 110 3743 60 2101 187 2869 Fragments arranged by size: 3743 2869 2101 187 110 60 BsrGI T'GTAC_A Cuts at: 96 4514 5235 96 Size: 4418 721 3931 Fragments arranged by size: 4418 3931 721 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9070 BssSI C'ACGA_G Cuts at: 2014 3381 3443 4560 7130 8142 2014 Size: 1367 62 1117 2570 1012 2942 Fragments arranged by size: 2942 2570 1367 1117 1012 62 BstAPI GCAn_nnn'nTGC Cuts at: 794 2590 2821 3017 3169 3220 4430 794 Size: 1796 231 196 152 51 1210 5434 Fragments arranged by size: 5434 1796 1210 231 196 152 51 BstBI TT'CG_AA Cuts at: 7874 7874 Size: 9070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9070 BstYI r'GATC_y Cuts at: 1972 2373 2758 2944 5244 6400 6767 7152 7169 Size: 401 385 186 2300 1156 367 385 17 Cuts at: 7169 8621 8707 8719 9065 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2300 1977 1452 1156 401 385 385 367 346 186 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5643 5942 5643 Size: 299 8771 BtgI C'CryG_G Cuts at: 513 1065 2328 3391 4142 5368 6180 513 Size: 552 1263 1063 751 1226 812 3403 Fragments arranged by size: 3403 1263 1226 1063 812 751 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8331 Fragments arranged by size: 8331 683 56 BtsI GCAGTG_nn' Cuts at: 2935 3288 4044 4290 5307 5394 5566 5690 6586 Size: 353 756 246 1017 87 172 124 896 Cuts at: 6586 7380 7400 2935 Size: 794 20 4605 Fragments arranged by size: 4605 1017 896 794 756 353 246 172 124 87 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9035 DraI TTT'AAA Cuts at: 6374 6374 Size: 9070 DraIII CAC_nnn'GTG Cuts at: 2407 4430 2407 Size: 2023 7047 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2018 3284 8077 809 Size: 507 702 1266 4793 1802 Fragments arranged by size: 4793 1802 1266 702 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3121 3543 3558 Size: 54 1425 50 275 18 1291 422 15 Cuts at: 3558 4365 5032 5119 5472 5525 6042 6517 7406 Size: 807 667 87 353 53 517 475 889 Cuts at: 7406 9035 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1291 889 807 667 517 475 422 353 275 87 54 53 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 6517 1537 Size: 275 4705 4090 Fragments arranged by size: 4705 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2639 2948 3651 3821 3885 4253 5888 6048 Size: 1286 309 703 170 64 368 1635 160 Cuts at: 6048 6110 6371 7000 1353 Size: 62 261 629 3423 Fragments arranged by size: 3423 1635 1286 703 629 368 309 261 170 160 64 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2020 5243 7657 8031 8177 387 Size: 1064 138 431 3223 2414 374 146 1280 Fragments arranged by size: 3223 2414 1280 1064 431 374 146 138 EcoICRI GAG'CTC Cuts at: 6160 6160 Size: 9070 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2370 2620 2874 2947 2972 3341 3632 Size: 393 738 250 254 73 25 369 291 Cuts at: 3632 3845 3909 4157 4189 4572 4702 4829 4848 Size: 213 64 248 32 383 130 127 19 Cuts at: 4848 4896 5108 5156 5195 5392 5555 6123 6176 Size: 48 212 48 39 197 163 568 53 Cuts at: 6176 6296 7133 7730 8517 8785 1239 Size: 120 837 597 787 268 1524 Fragments arranged by size: 1524 837 787 738 597 568 393 383 369 291 268 254 250 248 213 212 197 163 130 127 120 73 64 53 48 48 39 32 25 19 EcoNI CCTnn'n_nnAGG Cuts at: 5384 5849 6350 5384 Size: 465 501 8104 Fragments arranged by size: 8104 501 465 EcoO109I rG'GnC_Cy Cuts at: 2020 2044 2064 2170 3325 3326 3409 4232 4321 Size: 24 20 106 1155 1 83 823 89 Cuts at: 4321 4415 5413 2020 Size: 94 998 5677 Fragments arranged by size: 5677 1155 998 823 106 94 89 83 24 20 1 EcoRI G'AATT_C Cuts at: 6379 6757 6379 Size: 378 8692 EcoRV GAT'ATC Cuts at: 4101 5904 4101 Size: 1803 7267 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2977 3385 6221 2977 Size: 408 2836 5826 Fragments arranged by size: 5826 2836 408 FspI TGC'GCA Cuts at: 7576 7576 Size: 9070 HaeII r_GCGC'y Cuts at: 1678 6306 8217 1678 Size: 4628 1911 2531 Fragments arranged by size: 4628 2531 1911 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2616 2648 2974 Size: 32 135 32 722 32 290 32 326 Cuts at: 2974 3006 3046 3078 3521 3553 3590 3593 3622 Size: 32 40 32 443 32 37 3 29 Cuts at: 3622 3625 4007 4039 4216 4248 4260 4292 4373 Size: 3 382 32 177 32 12 32 81 Cuts at: 4373 4405 5497 5529 5621 5653 5663 5695 7711 Size: 32 1092 32 92 32 10 32 2016 Cuts at: 7711 7743 7785 7817 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2016 1092 722 443 382 326 290 177 135 92 81 42 40 37 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 12 10 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 4960 5341 5461 5928 6414 6660 Size: 988 281 3022 381 120 467 486 246 Cuts at: 6660 669 Size: 3079 Fragments arranged by size: 3079 3022 988 486 467 381 281 246 120 HindIII A'AGCT_T Cuts at: 748 748 Size: 9070 HpaI GTT'AAC Cuts at: 6660 6660 Size: 9070 KpnI G_GTAC'C Cuts at: 3469 6395 3469 Size: 2926 6144 MfeI C'AATT_G Cuts at: 6669 6669 Size: 9070 MluI A'CGCG_T Cuts at: 8946 8946 Size: 9070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 8184 8368 1305 Size: 729 6150 184 2007 Fragments arranged by size: 6150 2007 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3123 3545 5474 10 Size: 54 1425 343 1291 422 1929 3606 Fragments arranged by size: 3606 1929 1425 1291 422 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3671 3707 4157 4377 5373 5865 7028 Size: 648 2505 36 450 220 996 492 1163 Cuts at: 7028 7387 7546 518 Size: 359 159 2042 Fragments arranged by size: 2505 2042 1163 996 648 492 450 359 220 159 36 MspA1I CmG'CkG Cuts at: 1606 2144 2390 3220 3274 3614 3968 4306 4578 Size: 538 246 830 54 340 354 338 272 Cuts at: 4578 5581 5684 6143 6486 7163 8311 8556 1606 Size: 1003 103 459 343 677 1148 245 2120 Fragments arranged by size: 2120 1148 1003 830 677 538 459 354 343 340 338 272 246 245 103 54 NaeI GCC'GGC Cuts at: 5615 5615 Size: 9070 NcoI C'CATG_G Cuts at: 513 1065 3391 513 Size: 552 2326 6192 Fragments arranged by size: 6192 2326 552 NdeI CA'TA_TG Cuts at: 387 3346 387 Size: 2959 6111 NgoMIV G'CCGG_C Cuts at: 5613 5613 Size: 9070 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9070 NotI GC'GGCC_GC Cuts at: 6517 6517 Size: 9070 NsiI A_TGCA'T Cuts at: 2616 2616 Size: 9070 NspI r_CATG'y Cuts at: 1132 1229 2614 2618 2687 4346 6071 6085 6428 Size: 97 1385 4 69 1659 1725 14 343 Cuts at: 6428 7973 1132 Size: 1545 2229 Fragments arranged by size: 2229 1725 1659 1545 1385 343 97 69 14 4 PasI CC'CwG_GG Cuts at: 1348 2048 3913 3952 4236 1348 Size: 700 1865 39 284 6182 Fragments arranged by size: 6182 1865 700 284 39 PciI A'CATG_T Cuts at: 4342 6081 7969 4342 Size: 1739 1888 5443 Fragments arranged by size: 5443 1888 1739 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9070 PfoI T'CCnGG_A Cuts at: 4679 4679 Size: 9070 PmeI GTTT'AAAC Cuts at: 6374 6374 Size: 9070 PmlI CAC'GTG Cuts at: 6256 6256 Size: 9070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2018 2050 7139 7171 8652 8684 2018 Size: 32 5089 32 1481 32 2404 Fragments arranged by size: 5089 2404 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2044 2170 4232 4415 2020 Size: 24 126 2062 183 6675 Fragments arranged by size: 6675 2062 183 126 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9070 PsiI TTA'TAA Cuts at: 6640 6640 Size: 9070 PspOMI G'GGCC_C Cuts at: 3325 4321 3325 Size: 996 8074 PspXI vC'TCGA_Gb Cuts at: 3774 5879 3774 Size: 2105 6965 PstI C_TGCA'G Cuts at: 830 2817 3401 3474 4285 4708 4938 5097 5598 Size: 1987 584 73 811 423 230 159 501 Cuts at: 5598 6219 6422 830 Size: 621 203 3478 Fragments arranged by size: 3478 1987 811 621 584 501 423 230 203 159 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9070 PvuII CAG'CTG Cuts at: 2144 3220 3274 3614 3968 4306 4578 5684 6143 Size: 1076 54 340 354 338 272 1106 459 Cuts at: 6143 2144 Size: 5071 Fragments arranged by size: 5071 1106 1076 459 354 340 338 272 54 SacI G_AGCT'C Cuts at: 6162 6162 Size: 9070 SalI G'TCGA_C Cuts at: 1936 5459 6412 1936 Size: 3523 953 4594 Fragments arranged by size: 4594 3523 953 SanDI GG'GwC_CC Cuts at: 2020 4232 2020 Size: 2212 6858 SapI GCTCTTCn'nnn_ Cuts at: 2639 3651 6110 2639 Size: 1012 2459 5599 Fragments arranged by size: 5599 2459 1012 SbfI CC_TGCA'GG Cuts at: 3474 4938 6422 3474 Size: 1464 1484 6122 Fragments arranged by size: 6122 1484 1464 ScaI AGT'ACT Cuts at: 7318 9030 7318 Size: 1712 7358 SexAI A'CCwGG_T Cuts at: 4426 4426 Size: 9070 SfcI C'TryA_G Cuts at: 826 1045 2813 3397 3470 3576 4281 4474 4704 Size: 219 1768 584 73 106 705 193 230 Cuts at: 4704 4934 5093 5594 6215 6418 7553 8234 8425 Size: 230 159 501 621 203 1135 681 191 Cuts at: 8425 826 Size: 1471 Fragments arranged by size: 1768 1471 1135 705 681 621 584 501 230 230 219 203 193 191 159 106 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9070 SmaI CCC'GGG Cuts at: 1872 5588 6397 1872 Size: 3716 809 4545 Fragments arranged by size: 4545 3716 809 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 2311 3566 3774 4987 5879 Size: 197 926 246 122 1255 208 1213 892 Cuts at: 5879 6161 7174 8075 8337 820 Size: 282 1013 901 262 1553 Fragments arranged by size: 1553 1255 1213 1013 926 901 892 282 262 246 208 197 122 SnaBI TAC'GTA Cuts at: 493 6861 493 Size: 6368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9070 SphI G_CATG'C Cuts at: 1132 2618 2687 6428 1132 Size: 1486 69 3741 3774 Fragments arranged by size: 3774 3741 1486 69 SspI AAT'ATT Cuts at: 5 52 6994 5 Size: 47 6942 2081 Fragments arranged by size: 6942 2081 47 StuI AGG'CCT Cuts at: 1517 2258 3365 3733 3875 4593 6059 1517 Size: 741 1107 368 142 718 1466 4528 Fragments arranged by size: 4528 1466 1107 741 718 368 142 StyI C'CwwG_G Cuts at: 513 1065 3391 6509 513 Size: 552 2326 3118 3074 Fragments arranged by size: 3118 3074 2326 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3223 3504 4367 5677 7097 7114 7267 Size: 1255 1643 281 863 1310 1420 17 153 Cuts at: 7267 7452 7897 325 Size: 185 445 1498 Fragments arranged by size: 1643 1498 1420 1310 1255 863 445 281 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2809 2857 3855 Size: 274 80 33 51 1443 832 48 998 Cuts at: 3855 4514 5235 6084 7316 9028 96 Size: 659 721 849 1232 1712 138 Fragments arranged by size: 1712 1443 1232 998 849 832 721 659 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4249 4281 5114 5146 9030 9062 4249 Size: 32 833 32 3884 32 4257 Fragments arranged by size: 4257 3884 833 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9070 XbaI T'CTAG_A Cuts at: 6406 6406 Size: 9070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3553 3553 Size: 9070 XhoI C'TCGA_G Cuts at: 1943 3774 5879 1943 Size: 1831 2105 5134 Fragments arranged by size: 5134 2105 1831 XmaI C'CCGG_G Cuts at: 1870 5586 6395 1870 Size: 3716 809 4545 Fragments arranged by size: 4545 3716 809 XmnI GAAnn'nnTTC Cuts at: 2957 4776 7199 2957 Size: 1819 2423 4828 Fragments arranged by size: 4828 2423 1819 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2692 4176 6878 276 Size: 53 83 186 936 1158 1484 2702 2468 Fragments arranged by size: 2702 2468 1484 1158 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I FseI FspAI KasI NarI NruI PacI PsrI RsrII SacII SfiI SfoI SrfI SwaI