Restriction map for
pFN21AE6564
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MluI
MroI MslI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StyI SwaI Tsp45I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 343 GACGCC G[GA]^CG[CT]C
AflII 484 CTTAAG C^TTAAG
AspHI 250 GTGCTC G[AT]GC[AT]^C
AvaI 197 CCCGAG C^[CT]CG[GA]G
BalI 389 TGGCCA TGG^CCA
BamHI 35 GGATCC G^GATCC
BanI 504 GGCACC G^G[CT][GA]CC
BbvCI 442 GCTGAGG CCTCAGC(-5/-2)
BfaI 190 CTAG C^TAG
BfrI 484 CTTAAG C^TTAAG
BlpI 322 GCTGAGC GC^T[ACGT]AGC
BmyI 250 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 322 GCTGAGC GC^T[ACGT]AGC
BsaHI 343 GACGCC G[GA]^CG[CT]C
BsiHKAI 250 GTGCTC G[AT]GC[AT]^C
BsmFI 80 GGGAC GGGAC(10/14)
BsoBI 197 CCCGAG C^[CT]CG[GA]G
Bsp1286I 250 GTGCTC G[AGT]GC[ACT]^C
BstXI 228 CCAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 35 GGATCC [GA]^GATC[CT]
CelII 322 GCTGAGC GC^T[ACGT]AGC
DraIII 285 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 159 CCACGG C^C[GA][CT]GG
EcoNI 222 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 343 GACGC GACGC(5/10)
Hin1I 343 GACGCC G[GA]^CG[CT]C
MaeI 190 CTAG C^TAG
MboII 518 TCTTC GAAGA(8/7)
MflI 35 GGATCC [GA]^GATC[CT]
MluNI 389 TGGCCA TGG^CCA
MscI 389 TGGCCA TGG^CCA
MseI 485 TTAA T^TAA
NlaIII 405 CATG CATG^
PflMI 284 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 19 CAGCTG CAG^CTG
SbfI 435 CCTGCAGG CCTGCA^GG
SfaNI 170 GCATC GCATC(5/9)
SmlI 484 CTTAAG C^T[CT][GA]AG
Sse8387I 435 CCTGCAGG CCTGCA^GG
StuI 186 AGGCCT AGG^CCT
TaqI 173 TCGA T^CGA
TfiI 333 GATTC G^A[AT]TC
Tru9I 485 TTAA T^TAA
TspRI 393 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 173 TCGA T^CGA
Van91I 284 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 35 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 118 CGCG CG^CG
140 CGCG
AfaI 312 GTAC GT^AC
511 GTAC
AlwI 36 GATCC GGATC(4/5)
35 GGATC
AvaII 102 GGACC G^G[AT]CC
447 GGTCC
BsrI 240 ACTGG ACTGG(1/-1)
394 ACTGG
BstF5I 255 CATCC GGATG(2/0)
378 GGATG
BstUI 118 CGCG CG^CG
140 CGCG
CfoI 119 GCGC GCG^C
139 GCGC
DdeI 323 CTGAG C^T[ACGT]AG
443 CTGAG
DpnII 36 GATC ^GATC
382 GATC
EaeI 2 TGGCCG [CT]^GGCC[GA]
389 TGGCCA
FauI 106 CCCGC CCCGC(4/6)
264 GCGGG
FokI 255 CATCC GGATG(9/13)
378 GGATG
HhaI 119 GCGC GCG^C
139 GCGC
HinP1I 119 GCGC G^CGC
139 GCGC
MboI 36 GATC ^GATC
382 GATC
MspA1I 19 CAGCTG C[AC]G^C[GT]G
31 CAGCGG
MvnI 118 CGCG CG^CG
140 CGCG
NdeII 36 GATC ^GATC
382 GATC
PleI 7 GACTC GAGTC(4/5)
193 GACTC
PpuMI 101 AGGACCC [GA]G^G[AT]CC[CT]
446 AGGTCCT
RsaI 312 GTAC GT^AC
511 GTAC
Sau3AI 36 GATC ^GATC
382 GATC
XcmI 59 CCACCCCAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
125 CCATCGGCCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsiYI BslI BssKI BstNI Cac8I
Cfr13I DraII EcoO109I EcoRII Fnu4HI
HaeIII HinfI ItaI MnlI MvaI
MwoI NlaIV PstI Sau96I ScrFI
SfcI TseI