(Circular) (Six-base) MAPSORT of: pFN21AE6564.seq Check: 2443 from: 1 to: 5245 pFN21AE6564 5245 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3055 278 Size: 53 83 186 936 1519 2468 Fragments arranged by size: 2468 1519 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2588 1937 Size: 651 4594 Acc65I G'GTAC_C Cuts at: 2566 2566 Size: 5245 AclI AA'CG_TT Cuts at: 1563 3372 3745 1563 Size: 1809 373 3063 Fragments arranged by size: 3063 1809 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2174 3308 4692 4960 1632 Size: 491 51 1134 1384 268 1917 Fragments arranged by size: 1917 1384 1134 491 268 51 AflII C'TTAA_G Cuts at: 820 1017 2489 820 Size: 197 1472 3576 Fragments arranged by size: 3576 1472 197 AflIII A'CryG_T Cuts at: 1203 4144 5121 1203 Size: 2941 977 1327 Fragments arranged by size: 2941 1327 977 AgeI A'CCGG_T Cuts at: 4076 4076 Size: 5245 AhdI GACnn_n'nnGTC Cuts at: 3974 3974 Size: 5245 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5245 AlwNI CAG_nnn'CTG Cuts at: 2988 4560 2988 Size: 1572 3673 ApaLI G'TGCA_C Cuts at: 3302 4458 3302 Size: 1156 4089 ApoI r'AATT_y Cuts at: 1493 2554 2780 2932 5041 1493 Size: 1061 226 152 2109 1697 Fragments arranged by size: 2109 1697 1061 226 152 AseI AT'TA_AT Cuts at: 160 1032 3799 160 Size: 872 2767 1606 Fragments arranged by size: 2767 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5245 AvaI C'yCGr_G Cuts at: 1870 1943 2202 2570 1870 Size: 73 259 368 4545 Fragments arranged by size: 4545 368 259 73 BamHI G'GATC_C Cuts at: 2040 2575 2942 2040 Size: 535 367 4343 Fragments arranged by size: 4343 535 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2509 2566 4021 618 Size: 325 217 627 722 57 1455 1842 Fragments arranged by size: 1842 1455 722 627 325 217 57 BanII G_rGCy'C Cuts at: 1391 2564 1391 Size: 1173 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4274 BbvCI CC'TCA_GC Cuts at: 2448 2448 Size: 5245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3436 3470 1903 Size: 34 1499 34 3678 Fragments arranged by size: 3678 1499 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3137 4347 3137 Size: 1210 4035 BclI T'GATC_A Cuts at: 1548 1548 Size: 5245 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3856 136 Size: 107 122 71 631 605 2184 1525 Fragments arranged by size: 2184 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5240 5240 Size: 5245 BlpI GC'TnA_GC Cuts at: 2328 2662 2328 Size: 334 4911 Bme1580I G_kGCm'C Cuts at: 1790 3306 4462 1790 Size: 1516 1156 2573 Fragments arranged by size: 2573 1516 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5245 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2255 2976 3934 Size: 416 53 83 399 24 838 721 958 Cuts at: 3934 442 Size: 1753 Fragments arranged by size: 1753 958 838 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5245 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2228 2500 2228 Size: 272 4973 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 2354 2375 2477 3905 1239 Size: 1094 21 21 102 1428 2579 Fragments arranged by size: 2579 1428 1094 102 21 21 Bpu10I CC'TnA_GC Cuts at: 2448 2448 Size: 5245 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3370 4235 4533 3370 Size: 865 298 4082 Fragments arranged by size: 4082 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3908 882 Size: 617 2409 2219 Fragments arranged by size: 2409 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3036 493 Size: 711 1832 2702 Fragments arranged by size: 2702 1832 711 BsaBI GATnn'nnATC Cuts at: 5239 5239 Size: 5245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 3052 3434 4069 Size: 53 83 186 936 816 703 382 635 Cuts at: 4069 275 Size: 1451 Fragments arranged by size: 1451 936 816 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3678 4076 4350 4497 4908 3678 Size: 398 274 147 411 4015 Fragments arranged by size: 4015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2099 2129 1178 Size: 30 165 30 696 30 4294 Fragments arranged by size: 4294 696 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5245 BseYI C'CCAG_C Cuts at: 2431 4448 4944 2431 Size: 2017 496 2732 Fragments arranged by size: 2732 2017 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4672 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2695 3456 4484 1540 Size: 275 186 694 761 1028 2301 Fragments arranged by size: 2301 1028 761 694 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 3306 3391 4462 2259 Size: 1047 85 1071 3042 Fragments arranged by size: 3042 1071 1047 85 BsmI GAATG_Cn' Cuts at: 2756 2849 2756 Size: 93 5152 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2564 3306 3391 4462 1391 Size: 399 469 305 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 469 399 305 85 BspEI T'CCGG_A Cuts at: 4908 4908 Size: 5245 BspHI T'CATG_A Cuts at: 3134 4864 3134 Size: 1730 3515 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2600 844 Size: 1756 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3132 1394 Size: 429 1309 3507 Fragments arranged by size: 3507 1309 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3740 3914 65 Size: 1183 2492 174 1396 Fragments arranged by size: 2492 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3889 4076 1700 Size: 110 2079 187 2869 Fragments arranged by size: 2869 2079 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5245 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5245 BssSI C'ACGA_G Cuts at: 3305 4317 3305 Size: 1012 4233 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5245 BstBI TT'CG_AA Cuts at: 4049 4049 Size: 5245 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5245 BstXI CCAn_nnnn'nTGG Cuts at: 2240 2240 Size: 5245 BstYI r'GATC_y Cuts at: 1972 2040 2575 2942 3327 3344 4796 4882 4894 Size: 68 535 367 385 17 1452 86 12 Cuts at: 4894 5240 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 535 385 367 346 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2164 513 Size: 552 939 160 3594 Fragments arranged by size: 3594 939 552 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2170 487 Size: 683 56 944 3562 Fragments arranged by size: 3562 944 683 56 BtsI GCAGTG_nn' Cuts at: 2761 3555 3575 2761 Size: 794 20 4431 Fragments arranged by size: 4431 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5245 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5210 DraI TTT'AAA Cuts at: 2549 2549 Size: 5245 DraIII CAC_nnn'GTG Cuts at: 2295 2295 Size: 5245 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4252 809 Size: 507 2936 1802 Fragments arranged by size: 2936 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2394 2692 Size: 54 1425 50 275 18 177 387 298 Cuts at: 2692 3581 5210 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 387 298 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2692 1537 Size: 275 880 4090 Fragments arranged by size: 4090 880 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3175 1353 Size: 1822 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3832 4206 4352 387 Size: 1064 138 2243 374 146 1280 Fragments arranged by size: 2243 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2174 2333 2354 2375 2477 3308 Size: 393 491 51 159 21 21 102 831 Cuts at: 3308 3905 4692 4960 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 831 787 597 491 393 268 159 102 51 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 2231 2231 Size: 5245 EcoO109I rG'GnC_Cy Cuts at: 2107 2285 2452 2107 Size: 178 167 4900 Fragments arranged by size: 4900 178 167 EcoRI G'AATT_C Cuts at: 2554 2932 2554 Size: 378 4867 FspI TGC'GCA Cuts at: 3751 3751 Size: 5245 HaeII r_GCGC'y Cuts at: 1678 4392 1678 Size: 2714 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 3886 3918 3960 Size: 32 135 32 527 32 1755 32 42 Cuts at: 3960 3992 1373 Size: 32 2626 Fragments arranged by size: 2626 1755 527 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2589 2835 669 Size: 988 281 651 246 3079 Fragments arranged by size: 3079 988 651 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5245 HpaI GTT'AAC Cuts at: 2835 2835 Size: 5245 KpnI G_GTAC'C Cuts at: 2570 2570 Size: 5245 MfeI C'AATT_G Cuts at: 2844 2844 Size: 5245 MluI A'CGCG_T Cuts at: 5121 5121 Size: 5245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4359 4543 1305 Size: 3054 184 2007 Fragments arranged by size: 3054 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 10 Size: 54 1425 343 564 2859 Fragments arranged by size: 2859 1425 564 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3203 3562 3721 518 Size: 648 2037 359 159 2042 Fragments arranged by size: 2042 2037 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2038 2661 3338 4486 4731 1606 Size: 420 12 623 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 623 420 245 12 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3754 Fragments arranged by size: 3754 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5245 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5245 NotI GC'GGCC_GC Cuts at: 2692 2692 Size: 5245 NspI r_CATG'y Cuts at: 1132 1229 2603 4148 1132 Size: 97 1374 1545 2229 Fragments arranged by size: 2229 1545 1374 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5245 PciI A'CATG_T Cuts at: 4144 4144 Size: 5245 PflMI CCAn_nnn'nTGG Cuts at: 1108 2295 1108 Size: 1187 4058 PfoI T'CCnGG_A Cuts at: 2454 2454 Size: 5245 PmeI GTTT'AAAC Cuts at: 2549 2549 Size: 5245 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3314 3346 4827 4859 3314 Size: 32 1481 32 3700 Fragments arranged by size: 3700 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 2452 2107 Size: 345 4900 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5245 PsiI TTA'TAA Cuts at: 2815 2815 Size: 5245 PstI C_TGCA'G Cuts at: 830 2025 2445 2538 2597 830 Size: 1195 420 93 59 3478 Fragments arranged by size: 3478 1195 420 93 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5245 PvuII CAG'CTG Cuts at: 2026 2026 Size: 5245 SalI G'TCGA_C Cuts at: 1936 2587 1936 Size: 651 4594 SbfI CC_TGCA'GG Cuts at: 2445 2597 2445 Size: 152 5093 ScaI AGT'ACT Cuts at: 3493 5205 3493 Size: 1712 3533 SfcI C'TryA_G Cuts at: 826 1045 2021 2441 2534 2593 3728 4409 4600 Size: 219 976 420 93 59 1135 681 191 Cuts at: 4600 826 Size: 1471 Fragments arranged by size: 1471 1135 976 681 420 219 191 93 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5245 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5245 SmaI CCC'GGG Cuts at: 1872 2572 1872 Size: 700 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2489 3349 4250 4512 820 Size: 197 926 546 860 901 262 1553 Fragments arranged by size: 1553 926 901 860 546 262 197 SnaBI TAC'GTA Cuts at: 493 3036 493 Size: 2543 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5245 SphI G_CATG'C Cuts at: 1132 2603 1132 Size: 1471 3774 SspI AAT'ATT Cuts at: 5 52 3169 5 Size: 47 3117 2081 Fragments arranged by size: 3117 2081 47 StuI AGG'CCT Cuts at: 1517 2193 1517 Size: 676 4569 StyI C'CwwG_G Cuts at: 513 1065 2004 2684 513 Size: 552 939 680 3074 Fragments arranged by size: 3074 939 680 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 3289 3442 3627 4072 325 Size: 1255 1692 17 153 185 445 1498 Fragments arranged by size: 1692 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3491 5203 96 Size: 274 80 33 51 1443 1514 1712 138 Fragments arranged by size: 1712 1514 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2355 2387 5205 5237 2355 Size: 32 2818 32 2363 Fragments arranged by size: 2818 2363 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5245 XbaI T'CTAG_A Cuts at: 2581 2581 Size: 5245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 2137 2071 Size: 66 5179 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5245 XmaI C'CCGG_G Cuts at: 1870 2570 1870 Size: 700 4545 XmnI GAAnn'nnTTC Cuts at: 3374 3374 Size: 5245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3053 276 Size: 53 83 186 936 1519 2468 Fragments arranged by size: 2468 1519 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI