(Linear) (Six-base) MAPSORT of: KIEE6564.seq Check: 3487 from: 1 to: 540 KIEE6564.orf With 182 enzymes: SgfI * March 16, 2015 11:33 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 169 540 Size: 118 51 371 Fragments arranged by size: 371 118 51 AflII C'TTAA_G Cuts at: 0 484 540 Size: 484 56 AvaI C'yCGr_G Cuts at: 0 197 540 Size: 197 343 BamHI G'GATC_C Cuts at: 0 35 540 Size: 35 505 BanI G'GyrC_C Cuts at: 0 504 540 Size: 504 36 BbvCI CC'TCA_GC Cuts at: 0 443 540 Size: 443 97 BlpI GC'TnA_GC Cuts at: 0 323 540 Size: 323 217 BmrI ACTGGGnnnn_n' Cuts at: 0 250 540 Size: 250 290 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 223 495 540 Size: 223 272 45 Fragments arranged by size: 272 223 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 349 370 472 540 Size: 328 21 21 102 68 Fragments arranged by size: 328 102 68 21 21 Bpu10I CC'TnA_GC Cuts at: 0 443 540 Size: 443 97 BsaHI Gr'CG_yC Cuts at: 0 344 540 Size: 344 196 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 94 124 540 Size: 94 30 416 Fragments arranged by size: 416 94 30 BseYI C'CCAG_C Cuts at: 0 426 540 Size: 426 114 BsiHKAI G_wGCw'C Cuts at: 0 254 540 Size: 254 286 Bsp1286I G_dGCh'C Cuts at: 0 254 540 Size: 254 286 BstXI CCAn_nnnn'nTGG Cuts at: 0 235 540 Size: 235 305 BstYI r'GATC_y Cuts at: 0 35 540 Size: 35 505 BtgI C'CryG_G Cuts at: 0 159 540 Size: 159 381 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 165 540 Size: 165 375 DraIII CAC_nnn'GTG Cuts at: 0 290 540 Size: 290 250 EaeI y'GGCC_r Cuts at: 0 2 389 540 Size: 2 387 151 Fragments arranged by size: 387 151 2 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 169 328 349 370 472 540 Size: 118 51 159 21 21 102 68 Fragments arranged by size: 159 118 102 68 51 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 226 540 Size: 226 314 EcoO109I rG'GnC_Cy Cuts at: 0 102 280 447 540 Size: 102 178 167 93 Fragments arranged by size: 178 167 102 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 94 126 540 Size: 94 32 414 Fragments arranged by size: 414 94 32 MscI TGG'CCA Cuts at: 0 391 540 Size: 391 149 MspA1I CmG'CkG Cuts at: 0 21 33 540 Size: 21 12 507 Fragments arranged by size: 507 21 12 PflMI CCAn_nnn'nTGG Cuts at: 0 290 540 Size: 290 250 PfoI T'CCnGG_A Cuts at: 0 449 540 Size: 449 91 PpuMI rG'GwC_Cy Cuts at: 0 102 447 540 Size: 102 345 93 Fragments arranged by size: 345 102 93 PstI C_TGCA'G Cuts at: 0 20 440 533 540 Size: 20 420 93 7 Fragments arranged by size: 420 93 20 7 PvuII CAG'CTG Cuts at: 0 21 540 Size: 21 519 SbfI CC_TGCA'GG Cuts at: 0 440 540 Size: 440 100 SfcI C'TryA_G Cuts at: 0 16 436 529 540 Size: 16 420 93 11 Fragments arranged by size: 420 93 16 11 SmlI C'TyrA_G Cuts at: 0 484 540 Size: 484 56 StuI AGG'CCT Cuts at: 0 188 540 Size: 188 352 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 350 382 540 Size: 350 32 158 Fragments arranged by size: 350 158 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 66 132 540 Size: 66 66 408 Fragments arranged by size: 408 66 66 Enzymes that do cut: AcuI AflII AvaI BamHI BanI BbvCI BlpI BmrI BplI BpmI Bpu10I BsaHI BsaXI BseYI BsiHKAI Bsp1286I BstXI BstYI BtgI BtgZI DraIII EaeI Eco57MI EcoNI EcoO109I Hin4I MscI MspA1I PflMI PfoI PpuMI PstI PvuII SbfI SfcI SmlI StuI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI