################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccI AccIII AclI AcsI AflII AhdI AocI Aor51HI ApoI AseI AsnI Asp700I AspEI AvrII BamHI BcgI BciVI BfaI BfrI BlnI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I HindIII MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PflMI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SexAI SfuI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tsp509I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 715 GACGTC GACGT^C Acc65I 746 GGTACC G^GTACC AflIII 1586 ACGTGT A^C[GA][CT]GT AgeI 2918 ACCGGT A^CCGGT AscI 2644 GGCGCGCC GG^CGCGCC Asp718I 746 GGTACC G^GTACC AspI 1464 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1628 TGGCCA TGG^CCA BbsI 718 GTCTTC GAAGAC(2/6) BbvCI 444 GCTGAGG CCTCAGC(-5/-2) BclI 704 TGATCA T^GATCA BglII 1142 AGATCT A^GATCT BlpI 1035 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1035 GCTGAGC GC^T[ACGT]AGC BpuAI 718 GTCTTC GAAGAC(2/6) BsaWI 2918 ACCGGT [AT]^CCGG[AT] CelII 1035 GCTGAGC GC^T[ACGT]AGC DrdI 2860 GACAAGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 704 TGATCA T^GATCA HpaI 148 GTTAAC GTT^AAC KpnI 746 GGTACC GGTAC^C MluNI 1628 TGGCCA TGG^CCA MscI 1628 TGGCCA TGG^CCA MseI 149 TTAA T^TAA NcoI 354 CCATGG C^CATGG NspI 929 ACATGC [GA]CATG^[CT] PaeR7I 2485 CTCGAG C^TCGAG PflFI 1464 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2918 ACCGGT A^CCGGT PvuII 1053 CAGCTG CAG^CTG SbfI 765 CCTGCAGG CCTGCA^GG ScaI 2510 AGTACT AGT^ACT SfiI 672 GGCCGGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 765 CCTGCAGG CCTGCA^GG Tru9I 149 TTAA T^TAA Tth111I 1464 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2485 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AviII 932 TGCGCA TGC^GCA 2213 TGCGCA BbrPI 627 CACGTG CAC^GTG 3010 CACGTG BsaI 850 GAGACC GGTCTC(1/5) 2086 GAGACC BsrI 1281 ACTGG ACTGG(1/-1) 885 CCAGT BstEII 525 GGTCACC G^GT[ACGT]ACC 1101 GGTCACC BstPI 525 GGTCACC G^GT[ACGT]ACC 1101 GGTCACC DraIII 598 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 2046 CACGCTGTG EarI 1705 CTCTTC CTCTTC(1/4) 238 GAAGAG EcoNI 465 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1296 CCTGGCCGAGG EcoO65I 525 GGTCACC G^GT[ACGT]ACC 1101 GGTCACC FseI 2174 GGCCGGCC GGCCGG^CC 2655 GGCCGGCC FspI 932 TGCGCA TGC^GCA 2213 TGCGCA HincII 148 GTTAAC GT[CT]^[GA]AC 1765 GTCAAC HindII 148 GTTAAC GT[CT]^[GA]AC 1765 GTCAAC Ksp632I 1705 CTCTTC CTCTTC(1/4) 238 GAAGAG MaeIII 526 GTCAC ^GT[ACGT]AC 1102 GTCAC PmaCI 627 CACGTG CAC^GTG 3010 CACGTG PmlI 627 CACGTG CAC^GTG 3010 CACGTG PstI 766 CTGCAG CTGCA^G 1660 CTGCAG SapI 238 GAAGAGC GCTCTTC(1/4) 1704 GCTCTTC SfaNI 740 GCATC GCATC(5/9) 1382 GCATC SmlI 1015 CTCAAG C^T[CT][GA]AG 2485 CTCGAG Tsp45I 526 GTCAC ^GT[GC]AC 1102 GTCAC TspRI 701 CAGTG CA[GC]TG[ACGT][ACGT]^ 886 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI RsaI SacII Sau3AI Sau96I ScrFI SfcI SfoI SgrAI SmaI StuI StyI TaqI TseI TthHB8I XhoII XmaCI XmaI