(Circular) (Six-base) MAPSORT of: pFN21AB9768.seq Check: 4969 from: 1 to: 7723 pFN21AB9768 7723 bp With 182 enzymes: SgfI * July 29, 2015 18:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2790 2790 Size: 7723 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2724 5533 278 Size: 53 83 186 936 1188 2809 2468 Fragments arranged by size: 2809 2468 1188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5066 1937 Size: 3129 4594 Acc65I G'GTAC_C Cuts at: 2751 5044 2751 Size: 2293 5430 AclI AA'CG_TT Cuts at: 1563 5850 6223 1563 Size: 4287 373 3063 Fragments arranged by size: 4287 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2145 3745 4063 5786 7170 7438 1632 Size: 513 1600 318 1723 1384 268 1917 Fragments arranged by size: 1917 1723 1600 1384 513 318 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7526 AflIII A'CryG_T Cuts at: 1203 3591 6622 7599 1203 Size: 2388 3031 977 1327 Fragments arranged by size: 3031 2388 1327 977 AgeI A'CCGG_T Cuts at: 4923 6554 4923 Size: 1631 6092 AhdI GACnn_n'nnGTC Cuts at: 6452 6452 Size: 7723 AleI CACnn'nnGTG Cuts at: 1166 2521 2705 3060 1166 Size: 1355 184 355 5829 Fragments arranged by size: 5829 1355 355 184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2539 1184 Size: 1355 6368 AlwNI CAG_nnn'CTG Cuts at: 2259 2470 2665 5466 7038 2259 Size: 211 195 2801 1572 2944 Fragments arranged by size: 2944 2801 1572 211 195 ApaI G_GGCC'C Cuts at: 2320 2354 3437 3453 3574 3603 3724 3926 2320 Size: 34 1083 16 121 29 121 202 6117 Fragments arranged by size: 6117 1083 202 121 121 34 29 16 ApaLI G'TGCA_C Cuts at: 3026 3653 3785 4103 4459 5780 6936 3026 Size: 627 132 318 356 1321 1156 3813 Fragments arranged by size: 3813 1321 1156 627 356 318 132 ApoI r'AATT_y Cuts at: 1493 5032 5258 5410 7519 1493 Size: 3539 226 152 2109 1697 Fragments arranged by size: 3539 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 4650 4650 Size: 7723 AseI AT'TA_AT Cuts at: 160 1032 6277 160 Size: 872 5245 1606 Fragments arranged by size: 5245 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 AvaI C'yCGr_G Cuts at: 1870 1943 2131 3715 3821 3919 3965 4009 4165 Size: 73 188 1584 106 98 46 44 156 Cuts at: 4165 4301 4490 4666 4739 4755 4823 5048 1870 Size: 136 189 176 73 16 68 225 4545 Fragments arranged by size: 4545 1584 225 189 188 176 156 136 106 98 73 73 68 46 44 16 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4918 4951 4918 Size: 33 7690 BamHI G'GATC_C Cuts at: 5053 5420 5053 Size: 367 7356 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2119 2542 2751 2810 Size: 325 217 627 303 29 423 209 59 Cuts at: 2810 3015 3265 3544 3610 3686 3853 4262 4410 Size: 205 250 279 66 76 167 409 148 Cuts at: 4410 4432 4846 4900 5044 6499 618 Size: 22 414 54 144 1455 1842 Fragments arranged by size: 1842 1455 627 423 414 409 325 303 279 250 217 209 205 167 148 144 76 66 59 54 29 22 BanII G_rGCy'C Cuts at: 1391 2138 2320 2354 2411 2586 2631 3369 3437 Size: 747 182 34 57 175 45 738 68 Cuts at: 3437 3453 3536 3574 3603 3711 3724 3926 4010 Size: 16 83 38 29 108 13 202 84 Cuts at: 4010 4429 4779 5042 1391 Size: 419 350 263 4072 Fragments arranged by size: 4072 747 738 419 350 263 202 182 175 108 84 83 68 57 45 38 34 29 16 13 BbeI G_GCGC'C Cuts at: 3269 3614 3690 3857 4266 4414 4850 4904 3269 Size: 345 76 167 409 148 436 54 6088 Fragments arranged by size: 6088 436 409 345 167 148 76 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2716 928 Size: 971 817 5935 Fragments arranged by size: 5935 971 817 BbvCI CC'TCA_GC Cuts at: 2450 2450 Size: 7723 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5914 5948 1903 Size: 34 3977 34 3678 Fragments arranged by size: 3977 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5615 6825 5615 Size: 1210 6513 BclI T'GATC_A Cuts at: 1548 2709 1548 Size: 1161 6562 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2684 2808 3132 Size: 107 122 71 631 605 1012 124 324 Cuts at: 3132 4502 6334 136 Size: 1370 1832 1525 Fragments arranged by size: 1832 1525 1370 1012 631 605 324 124 122 107 71 BglII A'GATC_T Cuts at: 3147 7718 3147 Size: 4571 3152 BlpI GC'TnA_GC Cuts at: 3041 5140 3041 Size: 2099 5624 Bme1580I G_kGCm'C Cuts at: 1790 2320 2354 2639 2813 3030 3437 3453 3549 Size: 530 34 285 174 217 407 16 96 Cuts at: 3549 3574 3603 3657 3724 3789 3926 4107 4463 Size: 25 29 54 67 65 137 181 356 Cuts at: 4463 4526 4895 5784 6940 1790 Size: 63 369 889 1156 2573 Fragments arranged by size: 2573 1156 889 530 407 369 356 285 217 181 174 137 96 67 65 63 54 34 29 25 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2884 5454 6412 Size: 416 53 83 399 24 1467 2570 958 Cuts at: 6412 442 Size: 1753 Fragments arranged by size: 2570 1753 1467 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7723 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3169 3169 Size: 7723 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2281 2300 2642 2945 3520 4634 6383 1239 Size: 1042 19 342 303 575 1114 1749 2579 Fragments arranged by size: 2579 1749 1114 1042 575 342 303 19 Bpu10I CC'TnA_GC Cuts at: 2450 2561 4394 2450 Size: 111 1833 5779 Fragments arranged by size: 5779 1833 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 5848 6713 7011 3005 Size: 2843 865 298 3717 Fragments arranged by size: 3717 2843 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2849 4085 6386 882 Size: 617 1350 1236 2301 2219 Fragments arranged by size: 2301 2219 1350 1236 617 BsaAI yAC'GTr Cuts at: 493 1204 2634 3592 3738 3784 5017 5514 493 Size: 711 1430 958 146 46 1233 497 2702 Fragments arranged by size: 2702 1430 1233 958 711 497 146 46 BsaBI GATnn'nnATC Cuts at: 7717 7717 Size: 7723 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2721 3266 3470 3611 Size: 53 83 186 936 1188 545 204 141 Cuts at: 3611 3687 3854 4231 4263 4411 4685 4847 4872 Size: 76 167 377 32 148 274 162 25 Cuts at: 4872 4901 5530 5912 6547 275 Size: 29 629 382 635 1451 Fragments arranged by size: 1451 1188 936 635 629 545 382 377 274 204 186 167 162 148 141 83 76 53 32 29 25 BsaWI w'CCGG_w Cuts at: 4923 6156 6554 6828 6975 7386 4923 Size: 1233 398 274 147 411 5260 Fragments arranged by size: 5260 1233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 2640 2670 1178 Size: 30 165 30 663 30 544 30 6231 Fragments arranged by size: 6231 663 544 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2561 3422 3953 4987 1186 Size: 901 474 861 531 1034 3922 Fragments arranged by size: 3922 1034 901 861 531 474 BseYI C'CCAG_C Cuts at: 2141 2410 2670 2909 3172 3556 3947 4043 4883 Size: 269 260 239 263 384 391 96 840 Cuts at: 4883 6926 7422 2141 Size: 2043 496 2442 Fragments arranged by size: 2442 2043 840 496 391 384 269 263 260 239 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2312 2545 2631 4235 1163 Size: 573 363 213 233 86 1604 4651 Fragments arranged by size: 4651 1604 573 363 233 213 86 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2580 2826 3985 4154 4158 4185 Size: 275 186 579 246 1159 169 4 27 Cuts at: 4185 4260 4377 4692 4696 4815 5173 5934 6962 Size: 75 117 315 4 119 358 761 1028 Cuts at: 6962 1540 Size: 2301 Fragments arranged by size: 2301 1159 1028 761 579 358 315 275 246 186 169 119 117 75 27 4 4 BsiHKAI G_wGCw'C Cuts at: 2571 3030 3657 3789 4107 4463 5016 5784 5869 Size: 459 627 132 318 356 553 768 85 Cuts at: 5869 6940 2571 Size: 1071 3354 Fragments arranged by size: 3354 1071 768 627 553 459 356 318 132 85 BsmI GAATG_Cn' Cuts at: 5234 5327 5234 Size: 93 7630 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2320 2354 2411 2571 2586 2631 Size: 399 348 182 34 57 160 15 45 Cuts at: 2631 2639 2813 3030 3369 3437 3453 3536 3549 Size: 8 174 217 339 68 16 83 13 Cuts at: 3549 3574 3603 3657 3711 3724 3789 3926 4010 Size: 25 29 54 54 13 65 137 84 Cuts at: 4010 4107 4429 4463 4526 4779 4895 5016 5042 Size: 97 322 34 63 253 116 121 26 Cuts at: 5042 5784 5869 6940 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 348 339 322 253 217 182 174 160 137 121 116 97 85 84 83 68 65 63 57 54 54 45 34 34 29 26 25 16 15 13 13 8 BspEI T'CCGG_A Cuts at: 7386 7386 Size: 7723 BspHI T'CATG_A Cuts at: 5612 7342 5612 Size: 1730 5993 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2778 2790 4617 5078 844 Size: 1934 12 1827 461 3489 Fragments arranged by size: 3489 1934 1827 461 12 BsrBI CCG'CTC Cuts at: 1394 1823 2265 2956 3013 3045 3332 4417 4712 Size: 429 442 691 57 32 287 1085 295 Cuts at: 4712 5610 1394 Size: 898 3507 Fragments arranged by size: 3507 1085 898 691 442 429 295 287 57 32 BsrDI GCAATG_nn' Cuts at: 65 1248 6218 6392 65 Size: 1183 4970 174 1396 Fragments arranged by size: 4970 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2284 2539 2813 3660 3689 4153 4180 Size: 110 474 255 274 847 29 464 27 Cuts at: 4180 4259 4435 4661 4687 4843 4897 4923 6367 Size: 79 176 226 26 156 54 26 1444 Cuts at: 6367 6554 1700 Size: 187 2869 Fragments arranged by size: 2869 1444 847 474 464 274 255 226 187 176 156 110 79 54 29 27 26 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7723 BssHII G'CGCG_C Cuts at: 1925 3696 3839 3873 3932 4355 4545 4650 1925 Size: 1771 143 34 59 423 190 105 4998 Fragments arranged by size: 4998 1771 423 190 143 105 59 34 BssSI C'ACGA_G Cuts at: 4088 4454 4519 5783 6795 4088 Size: 366 65 1264 1012 5016 Fragments arranged by size: 5016 1264 1012 366 65 BstAPI GCAn_nnn'nTGC Cuts at: 794 2904 4056 4467 4639 794 Size: 2110 1152 411 172 3878 Fragments arranged by size: 3878 2110 1152 411 172 BstBI TT'CG_AA Cuts at: 6527 6527 Size: 7723 BstEII G'GTnAC_C Cuts at: 1184 2530 3106 1184 Size: 1346 576 5801 Fragments arranged by size: 5801 1346 576 BstXI CCAn_nnnn'nTGG Cuts at: 2101 2206 2923 3062 3118 2101 Size: 105 717 139 56 6706 Fragments arranged by size: 6706 717 139 105 56 BstYI r'GATC_y Cuts at: 1972 2496 2928 3147 3627 5053 5420 5805 5822 Size: 524 432 219 480 1426 367 385 17 Cuts at: 5822 7274 7360 7372 7718 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1426 524 480 432 385 367 346 219 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2115 2235 2334 2359 3249 4020 4310 Size: 552 1050 120 99 25 890 771 290 Cuts at: 4310 4371 4406 4696 4747 513 Size: 61 35 290 51 3489 Fragments arranged by size: 3489 1050 890 771 552 290 290 120 99 61 51 35 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3261 4957 487 Size: 683 56 2035 1696 3253 Fragments arranged by size: 3253 2035 1696 683 56 BtsI GCAGTG_nn' Cuts at: 2712 5239 6033 6053 2712 Size: 2527 794 20 4382 Fragments arranged by size: 4382 2527 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7723 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2722 2757 529 Size: 35 2158 35 5495 Fragments arranged by size: 5495 2158 35 35 DraI TTT'AAA Cuts at: 5027 5027 Size: 7723 DraIII CAC_nnn'GTG Cuts at: 2608 4056 2608 Size: 1448 6275 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4871 6730 809 Size: 507 3555 1859 1802 Fragments arranged by size: 3555 1859 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2577 2599 2823 Size: 54 1425 50 275 18 747 22 224 Cuts at: 2823 3303 3495 3564 3633 3883 3957 3982 4155 Size: 480 192 69 69 250 74 25 173 Cuts at: 4155 4182 4257 4285 4374 4443 4659 4689 4812 Size: 27 75 28 89 69 216 30 123 Cuts at: 4812 5170 6059 7688 8 Size: 358 889 1629 43 Fragments arranged by size: 1629 1425 889 747 480 358 275 250 224 216 192 173 123 89 75 74 69 69 69 54 50 43 30 28 27 25 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2577 2823 3982 4155 4182 4257 4374 Size: 275 765 246 1159 173 27 75 117 Cuts at: 4374 4689 4812 5170 1537 Size: 315 123 358 4090 Fragments arranged by size: 4090 1159 765 358 315 275 246 173 123 117 75 27 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 3716 5653 1353 Size: 885 1478 1937 3423 Fragments arranged by size: 3423 1937 1478 885 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2029 2414 4613 6310 6684 6830 Size: 1064 138 440 385 2199 1697 374 146 Cuts at: 6830 387 Size: 1280 Fragments arranged by size: 2199 1697 1280 1064 440 385 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 2281 2300 2642 2945 3520 3745 Size: 393 513 136 19 342 303 575 225 Cuts at: 3745 4063 4634 5786 6383 7170 7438 1239 Size: 318 571 1152 597 787 268 1524 Fragments arranged by size: 1524 1152 787 597 575 571 513 393 342 318 303 268 225 136 19 EcoNI CCTnn'n_nnAGG Cuts at: 2474 3305 2474 Size: 831 6892 EcoO109I rG'GnC_Cy Cuts at: 2195 2350 2467 3236 3298 3434 3450 3457 3570 Size: 155 117 769 62 136 16 7 113 Cuts at: 3570 3571 3720 3721 3923 4169 4606 4743 2195 Size: 1 149 1 202 246 437 137 5175 Fragments arranged by size: 5175 769 437 246 202 155 149 137 136 117 113 62 16 7 1 1 EcoRI G'AATT_C Cuts at: 5032 5410 5032 Size: 378 7345 FseI GG_CCGG'CC Cuts at: 4184 4665 4184 Size: 481 7242 FspI TGC'GCA Cuts at: 2939 4220 6229 2939 Size: 1281 2009 4433 Fragments arranged by size: 4433 2009 1281 FspAI rTGC'GCAy Cuts at: 4220 4220 Size: 7723 HaeII r_GCGC'y Cuts at: 1678 2916 3269 3614 3690 3735 3796 3857 4266 Size: 1238 353 345 76 45 61 61 409 Cuts at: 4266 4414 4442 4850 4904 6870 1678 Size: 148 28 408 54 1966 2531 Fragments arranged by size: 2531 1966 1238 409 408 353 345 148 76 61 61 54 45 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2313 2345 6364 Size: 32 135 32 569 32 140 32 4019 Cuts at: 6364 6396 6438 6470 1373 Size: 32 42 32 2626 Fragments arranged by size: 4019 2626 569 140 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2155 3772 5067 5313 669 Size: 988 281 217 1617 1295 246 3079 Fragments arranged by size: 3079 1617 1295 988 281 246 217 HindIII A'AGCT_T Cuts at: 748 748 Size: 7723 HpaI GTT'AAC Cuts at: 2155 5313 2155 Size: 3158 4565 KasI G'GCGC_C Cuts at: 3265 3610 3686 3853 4262 4410 4846 4900 3265 Size: 345 76 167 409 148 436 54 6088 Fragments arranged by size: 6088 436 409 345 167 148 76 54 KpnI G_GTAC'C Cuts at: 2755 5048 2755 Size: 2293 5430 MfeI C'AATT_G Cuts at: 5322 5322 Size: 7723 MluI A'CGCG_T Cuts at: 7599 7599 Size: 7723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6837 7021 1305 Size: 5532 184 2007 Fragments arranged by size: 5532 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3635 10 Size: 54 1425 343 1803 4098 Fragments arranged by size: 4098 1803 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2364 2521 2705 3060 3392 5681 6040 Size: 648 1198 157 184 355 332 2289 359 Cuts at: 6040 6199 518 Size: 159 2042 Fragments arranged by size: 2289 2042 1198 648 359 355 332 184 159 157 MspA1I CmG'CkG Cuts at: 1606 2170 2949 3060 3251 4312 4373 4408 4529 Size: 564 779 111 191 1061 61 35 121 Cuts at: 4529 4698 4906 5139 5816 6964 7209 1606 Size: 169 208 233 677 1148 245 2120 Fragments arranged by size: 2120 1148 1061 779 677 564 245 233 208 191 169 121 111 61 35 NaeI GCC'GGC Cuts at: 3662 3691 4182 4261 4663 4689 4845 4899 3662 Size: 29 491 79 402 26 156 54 6486 Fragments arranged by size: 6486 491 402 156 79 54 29 26 NarI GG'CG_CC Cuts at: 3266 3611 3687 3854 4263 4411 4847 4901 3266 Size: 345 76 167 409 148 436 54 6088 Fragments arranged by size: 6088 436 409 345 167 148 76 54 NcoI C'CATG_G Cuts at: 513 1065 2359 513 Size: 552 1294 5877 Fragments arranged by size: 5877 1294 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7723 NgoMIV G'CCGG_C Cuts at: 3660 3689 4180 4259 4661 4687 4843 4897 3660 Size: 29 491 79 402 26 156 54 6486 Fragments arranged by size: 6486 491 402 156 79 54 29 26 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7723 NotI GC'GGCC_GC Cuts at: 5170 5170 Size: 7723 NspI r_CATG'y Cuts at: 1132 1229 2938 5081 6626 1132 Size: 97 1709 2143 1545 2229 Fragments arranged by size: 2229 2143 1709 1545 97 PasI CC'CwG_GG Cuts at: 1348 4175 1348 Size: 2827 4896 PciI A'CATG_T Cuts at: 6622 6622 Size: 7723 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7723 PmeI GTTT'AAAC Cuts at: 5027 5027 Size: 7723 PmlI CAC'GTG Cuts at: 2634 5017 2634 Size: 2383 5340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5792 5824 7305 7337 5792 Size: 32 1481 32 6178 Fragments arranged by size: 6178 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4169 4606 4743 4169 Size: 437 137 7149 Fragments arranged by size: 7149 437 137 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7723 PsiI TTA'TAA Cuts at: 5293 5293 Size: 7723 PspOMI G'GGCC_C Cuts at: 2316 2350 3433 3449 3570 3599 3720 3922 2316 Size: 34 1083 16 121 29 121 202 6117 Fragments arranged by size: 6117 1083 202 121 121 34 29 16 PspXI vC'TCGA_Gb Cuts at: 4490 4490 Size: 7723 PstI C_TGCA'G Cuts at: 830 2775 3669 5075 830 Size: 1945 894 1406 3478 Fragments arranged by size: 3478 1945 1406 894 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7723 PvuII CAG'CTG Cuts at: 3060 3060 Size: 7723 SacII CC_GC'GG Cuts at: 3252 4313 4374 4409 4699 3252 Size: 1061 61 35 290 6276 Fragments arranged by size: 6276 1061 290 61 35 SalI G'TCGA_C Cuts at: 1936 5065 1936 Size: 3129 4594 SanDI GG'GwC_CC Cuts at: 4169 4743 4169 Size: 574 7149 SapI GCTCTTCn'nnn_ Cuts at: 2238 3716 2238 Size: 1478 6245 SbfI CC_TGCA'GG Cuts at: 2775 5075 2775 Size: 2300 5423 ScaI AGT'ACT Cuts at: 4517 5971 7683 4517 Size: 1454 1712 4557 Fragments arranged by size: 4557 1712 1454 SfcI C'TryA_G Cuts at: 826 1045 2771 3094 3665 5071 6206 6887 7078 Size: 219 1726 323 571 1406 1135 681 191 Cuts at: 7078 826 Size: 1471 Fragments arranged by size: 1726 1471 1406 1135 681 571 323 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2684 2684 Size: 7723 SfoI GGC'GCC Cuts at: 3267 3612 3688 3855 4264 4412 4848 4902 3267 Size: 345 76 167 409 148 436 54 6088 Fragments arranged by size: 6088 436 409 345 167 148 76 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 SgrAI Cr'CCGG_yG Cuts at: 1700 3689 4435 4843 4897 1700 Size: 1989 746 408 54 4526 Fragments arranged by size: 4526 1989 746 408 54 SmaI CCC'GGG Cuts at: 1872 3717 3921 4011 4167 4668 4741 4757 5050 Size: 1845 204 90 156 501 73 16 293 Cuts at: 5050 1872 Size: 4545 Fragments arranged by size: 4545 1845 501 293 204 156 90 73 16 SmlI C'TyrA_G Cuts at: 820 1017 1943 3020 4490 5827 6728 6990 820 Size: 197 926 1077 1470 1337 901 262 1553 Fragments arranged by size: 1553 1470 1337 1077 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5514 493 Size: 5021 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7723 SphI G_CATG'C Cuts at: 1132 5081 1132 Size: 3949 3774 SspI AAT'ATT Cuts at: 5 52 5647 5 Size: 47 5595 2081 Fragments arranged by size: 5595 2081 47 StuI AGG'CCT Cuts at: 1517 2441 4064 4496 1517 Size: 924 1623 432 4744 Fragments arranged by size: 4744 1623 924 432 StyI C'CwwG_G Cuts at: 513 1065 2199 2359 2997 3138 3726 3849 5162 Size: 552 1134 160 638 141 588 123 1313 Cuts at: 5162 513 Size: 3074 Fragments arranged by size: 3074 1313 1134 638 588 552 160 141 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 3186 5750 5767 5920 6105 6550 Size: 1255 498 1108 2564 17 153 185 445 Cuts at: 6550 325 Size: 1498 Fragments arranged by size: 2564 1498 1255 1108 498 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4515 5969 7681 Size: 274 80 33 51 1443 2538 1454 1712 Cuts at: 7681 96 Size: 138 Fragments arranged by size: 2538 1712 1454 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2640 2672 7683 7715 2640 Size: 32 5011 32 2648 Fragments arranged by size: 5011 2648 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3472 1316 Size: 2156 5567 XbaI T'CTAG_A Cuts at: 5059 5059 Size: 7723 XhoI C'TCGA_G Cuts at: 1943 4490 1943 Size: 2547 5176 XmaI C'CCGG_G Cuts at: 1870 3715 3919 4009 4165 4666 4739 4755 5048 Size: 1845 204 90 156 501 73 16 293 Cuts at: 5048 1870 Size: 4545 Fragments arranged by size: 4545 1845 501 293 204 156 90 73 16 XmnI GAAnn'nnTTC Cuts at: 5852 5852 Size: 7723 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2722 5531 276 Size: 53 83 186 936 1188 2809 2468 Fragments arranged by size: 2809 2468 1188 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI NruI NsiI PacI PfoI PsrI RsrII SacI SexAI SrfI SwaI XcmI