(Linear) (Six-base) MAPSORT of: KIBB9768.seq Check: 3989 from: 1 to: 3018 KIBB9768.orf With 182 enzymes: SgfI * July 29, 2015 17:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 785 3018 Size: 785 2233 AatII G_ACGT'C Cuts at: 0 719 3018 Size: 719 2299 Acc65I G'GTAC_C Cuts at: 0 746 3018 Size: 746 2272 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 140 1740 2058 3018 Size: 140 1600 318 960 Fragments arranged by size: 1600 960 318 140 AflIII A'CryG_T Cuts at: 0 1586 3018 Size: 1586 1432 AgeI A'CCGG_T Cuts at: 0 2918 3018 Size: 2918 100 AleI CACnn'nnGTG Cuts at: 0 516 700 1055 3018 Size: 516 184 355 1963 Fragments arranged by size: 1963 516 355 184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 534 3018 Size: 534 2484 AlwNI CAG_nnn'CTG Cuts at: 0 254 465 660 3018 Size: 254 211 195 2358 Fragments arranged by size: 2358 254 211 195 ApaI G_GGCC'C Cuts at: 0 315 349 1432 1448 1569 1598 1719 1921 Size: 315 34 1083 16 121 29 121 202 Cuts at: 1921 3018 Size: 1097 Fragments arranged by size: 1097 1083 315 202 121 121 34 29 16 ApaLI G'TGCA_C Cuts at: 0 1021 1648 1780 2098 2454 3018 Size: 1021 627 132 318 356 564 Fragments arranged by size: 1021 627 564 356 318 132 AscI GG'CGCG_CC Cuts at: 0 2645 3018 Size: 2645 373 AvaI C'yCGr_G Cuts at: 0 126 1710 1816 1914 1960 2004 2160 2296 Size: 126 1584 106 98 46 44 156 136 Cuts at: 2296 2485 2661 2734 2750 2818 3018 Size: 189 176 73 16 68 200 Fragments arranged by size: 1584 200 189 176 156 136 126 106 98 73 68 46 44 16 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2913 2946 3018 Size: 2913 33 72 Fragments arranged by size: 2913 72 33 BanI G'GyrC_C Cuts at: 0 85 114 537 746 805 1010 1260 1539 Size: 85 29 423 209 59 205 250 279 Cuts at: 1539 1605 1681 1848 2257 2405 2427 2841 2895 Size: 66 76 167 409 148 22 414 54 Cuts at: 2895 3018 Size: 123 Fragments arranged by size: 423 414 409 279 250 209 205 167 148 123 85 76 66 59 54 29 22 BanII G_rGCy'C Cuts at: 0 133 315 349 406 581 626 1364 1432 Size: 133 182 34 57 175 45 738 68 Cuts at: 1432 1448 1531 1569 1598 1706 1719 1921 2005 Size: 16 83 38 29 108 13 202 84 Cuts at: 2005 2424 2774 3018 Size: 419 350 244 Fragments arranged by size: 738 419 350 244 202 182 175 133 108 84 83 68 57 45 38 34 29 16 13 BbeI G_GCGC'C Cuts at: 0 1264 1609 1685 1852 2261 2409 2845 2899 Size: 1264 345 76 167 409 148 436 54 Cuts at: 2899 3018 Size: 119 Fragments arranged by size: 1264 436 409 345 167 148 119 76 54 BbsI GAAGACnn'nnnn_ Cuts at: 0 711 3018 Size: 711 2307 BbvCI CC'TCA_GC Cuts at: 0 445 3018 Size: 445 2573 BclI T'GATC_A Cuts at: 0 704 3018 Size: 704 2314 BglI GCCn_nnn'nGGC Cuts at: 0 679 803 1127 2497 3018 Size: 679 124 324 1370 521 Fragments arranged by size: 1370 679 521 324 124 BglII A'GATC_T Cuts at: 0 1142 3018 Size: 1142 1876 BlpI GC'TnA_GC Cuts at: 0 1036 3018 Size: 1036 1982 Bme1580I G_kGCm'C Cuts at: 0 315 349 634 808 1025 1432 1448 1544 Size: 315 34 285 174 217 407 16 96 Cuts at: 1544 1569 1598 1652 1719 1784 1921 2102 2458 Size: 25 29 54 67 65 137 181 356 Cuts at: 2458 2521 2890 3018 Size: 63 369 128 Fragments arranged by size: 407 369 356 315 285 217 181 174 137 128 96 67 65 63 54 34 29 25 16 BmrI ACTGGGnnnn_n' Cuts at: 0 879 3018 Size: 879 2139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1164 3018 Size: 1164 1854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 276 295 637 940 1515 2629 3018 Size: 276 19 342 303 575 1114 389 Fragments arranged by size: 1114 575 389 342 303 276 19 Bpu10I CC'TnA_GC Cuts at: 0 445 556 2389 3018 Size: 445 111 1833 629 Fragments arranged by size: 1833 629 445 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1000 3018 Size: 1000 2018 BsaI GGTCTCn'nnnn_ Cuts at: 0 844 2080 3018 Size: 844 1236 938 Fragments arranged by size: 1236 938 844 BsaAI yAC'GTr Cuts at: 0 629 1587 1733 1779 3012 3018 Size: 629 958 146 46 1233 6 Fragments arranged by size: 1233 958 629 146 46 6 BsaHI Gr'CG_yC Cuts at: 0 716 1261 1465 1606 1682 1849 2226 2258 Size: 716 545 204 141 76 167 377 32 Cuts at: 2258 2406 2680 2842 2867 2896 3018 Size: 148 274 162 25 29 122 Fragments arranged by size: 716 545 377 274 204 167 162 148 141 122 76 32 29 25 BsaWI w'CCGG_w Cuts at: 0 2918 3018 Size: 2918 100 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 61 91 635 665 3018 Size: 61 30 544 30 2353 Fragments arranged by size: 2353 544 61 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 82 556 1417 1948 2982 3018 Size: 82 474 861 531 1034 36 Fragments arranged by size: 1034 861 531 474 82 36 BseYI C'CCAG_C Cuts at: 0 136 405 665 904 1167 1551 1942 2038 Size: 136 269 260 239 263 384 391 96 Cuts at: 2038 2878 3018 Size: 840 140 Fragments arranged by size: 840 391 384 269 263 260 239 140 136 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 307 540 626 2230 3018 Size: 94 213 233 86 1604 788 Fragments arranged by size: 1604 788 233 213 94 86 BsiEI CG_ry'CG Cuts at: 0 575 821 1980 2149 2153 2180 2255 2372 Size: 575 246 1159 169 4 27 75 117 Cuts at: 2372 2687 2691 2810 3018 Size: 315 4 119 208 Fragments arranged by size: 1159 575 315 246 208 169 119 117 75 27 4 4 BsiHKAI G_wGCw'C Cuts at: 0 566 1025 1652 1784 2102 2458 3011 3018 Size: 566 459 627 132 318 356 553 7 Fragments arranged by size: 627 566 553 459 356 318 132 7 Bsp1286I G_dGCh'C Cuts at: 0 133 315 349 406 566 581 626 634 Size: 133 182 34 57 160 15 45 8 Cuts at: 634 808 1025 1364 1432 1448 1531 1544 1569 Size: 174 217 339 68 16 83 13 25 Cuts at: 1569 1598 1652 1706 1719 1784 1921 2005 2102 Size: 29 54 54 13 65 137 84 97 Cuts at: 2102 2424 2458 2521 2774 2890 3011 3018 Size: 322 34 63 253 116 121 7 Fragments arranged by size: 339 322 253 217 182 174 160 137 133 121 116 97 84 83 68 65 63 57 54 54 45 34 34 29 25 16 15 13 13 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 773 785 2612 3018 Size: 773 12 1827 406 Fragments arranged by size: 1827 773 406 12 BsrBI CCG'CTC Cuts at: 0 260 951 1008 1040 1327 2412 2707 3018 Size: 260 691 57 32 287 1085 295 311 Fragments arranged by size: 1085 691 311 295 287 260 57 32 BsrFI r'CCGG_y Cuts at: 0 279 534 808 1655 1684 2148 2175 2254 Size: 279 255 274 847 29 464 27 79 Cuts at: 2254 2430 2656 2682 2838 2892 2918 3018 Size: 176 226 26 156 54 26 100 Fragments arranged by size: 847 464 279 274 255 226 176 156 100 79 54 29 27 26 26 BssHII G'CGCG_C Cuts at: 0 1691 1834 1868 1927 2350 2540 2645 3018 Size: 1691 143 34 59 423 190 105 373 Fragments arranged by size: 1691 423 373 190 143 105 59 34 BssSI C'ACGA_G Cuts at: 0 2083 2449 2514 3018 Size: 2083 366 65 504 Fragments arranged by size: 2083 504 366 65 BstAPI GCAn_nnn'nTGC Cuts at: 0 899 2051 2462 2634 3018 Size: 899 1152 411 172 384 Fragments arranged by size: 1152 899 411 384 172 BstEII G'GTnAC_C Cuts at: 0 525 1101 3018 Size: 525 576 1917 Fragments arranged by size: 1917 576 525 BstXI CCAn_nnnn'nTGG Cuts at: 0 96 201 918 1057 1113 3018 Size: 96 105 717 139 56 1905 Fragments arranged by size: 1905 717 139 105 96 56 BstYI r'GATC_y Cuts at: 0 491 923 1142 1622 3018 Size: 491 432 219 480 1396 Fragments arranged by size: 1396 491 480 432 219 BtgI C'CryG_G Cuts at: 0 110 230 329 354 1244 2015 2305 2366 Size: 110 120 99 25 890 771 290 61 Cuts at: 2366 2401 2691 2742 3018 Size: 35 290 51 276 Fragments arranged by size: 890 771 290 290 276 120 110 99 61 51 35 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1256 2952 3018 Size: 1256 1696 66 Fragments arranged by size: 1696 1256 66 BtsI GCAGTG_nn' Cuts at: 0 707 3018 Size: 707 2311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 717 752 3018 Size: 717 35 2266 Fragments arranged by size: 2266 717 35 DraIII CAC_nnn'GTG Cuts at: 0 603 2051 3018 Size: 603 1448 967 Fragments arranged by size: 1448 967 603 DrdI GACnn_nn'nnGTC Cuts at: 0 2866 3018 Size: 2866 152 EaeI y'GGCC_r Cuts at: 0 572 594 818 1298 1490 1559 1628 1878 Size: 572 22 224 480 192 69 69 250 Cuts at: 1878 1952 1977 2150 2177 2252 2280 2369 2438 Size: 74 25 173 27 75 28 89 69 Cuts at: 2438 2654 2684 2807 3018 Size: 216 30 123 211 Fragments arranged by size: 572 480 250 224 216 211 192 173 123 89 75 74 69 69 69 30 28 27 25 22 EagI C'GGCC_G Cuts at: 0 572 818 1977 2150 2177 2252 2369 2684 Size: 572 246 1159 173 27 75 117 315 Cuts at: 2684 2807 3018 Size: 123 211 Fragments arranged by size: 1159 572 315 246 211 173 123 117 75 27 EarI CTCTTCn'nnn_ Cuts at: 0 233 1711 3018 Size: 233 1478 1307 Fragments arranged by size: 1478 1307 233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 24 409 2608 3018 Size: 24 385 2199 410 Fragments arranged by size: 2199 410 385 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 140 276 295 637 940 1515 1740 2058 Size: 140 136 19 342 303 575 225 318 Cuts at: 2058 2629 3018 Size: 571 389 Fragments arranged by size: 575 571 389 342 318 303 225 140 136 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 469 1300 3018 Size: 469 831 1718 Fragments arranged by size: 1718 831 469 EcoO109I rG'GnC_Cy Cuts at: 0 190 345 462 1231 1293 1429 1445 1452 Size: 190 155 117 769 62 136 16 7 Cuts at: 1452 1565 1566 1715 1716 1918 2164 2601 2738 Size: 113 1 149 1 202 246 437 137 Cuts at: 2738 3018 Size: 280 Fragments arranged by size: 769 437 280 246 202 190 155 149 137 136 117 113 62 16 7 1 1 FseI GG_CCGG'CC Cuts at: 0 2179 2660 3018 Size: 2179 481 358 Fragments arranged by size: 2179 481 358 FspI TGC'GCA Cuts at: 0 934 2215 3018 Size: 934 1281 803 Fragments arranged by size: 1281 934 803 FspAI rTGC'GCAy Cuts at: 0 2215 3018 Size: 2215 803 HaeII r_GCGC'y Cuts at: 0 911 1264 1609 1685 1730 1791 1852 2261 Size: 911 353 345 76 45 61 61 409 Cuts at: 2261 2409 2437 2845 2899 3018 Size: 148 28 408 54 119 Fragments arranged by size: 911 409 408 353 345 148 119 76 61 61 54 45 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 136 168 308 340 3018 Size: 136 32 140 32 2678 Fragments arranged by size: 2678 140 136 32 32 HincII GTy'rAC Cuts at: 0 150 1767 3018 Size: 150 1617 1251 Fragments arranged by size: 1617 1251 150 HpaI GTT'AAC Cuts at: 0 150 3018 Size: 150 2868 KasI G'GCGC_C Cuts at: 0 1260 1605 1681 1848 2257 2405 2841 2895 Size: 1260 345 76 167 409 148 436 54 Cuts at: 2895 3018 Size: 123 Fragments arranged by size: 1260 436 409 345 167 148 123 76 54 KpnI G_GTAC'C Cuts at: 0 750 3018 Size: 750 2268 MscI TGG'CCA Cuts at: 0 1630 3018 Size: 1630 1388 MslI CAynn'nnrTG Cuts at: 0 359 516 700 1055 1387 3018 Size: 359 157 184 355 332 1631 Fragments arranged by size: 1631 359 355 332 184 157 MspA1I CmG'CkG Cuts at: 0 165 944 1055 1246 2307 2368 2403 2524 Size: 165 779 111 191 1061 61 35 121 Cuts at: 2524 2693 2901 3018 Size: 169 208 117 Fragments arranged by size: 1061 779 208 191 169 165 121 117 111 61 35 NaeI GCC'GGC Cuts at: 0 1657 1686 2177 2256 2658 2684 2840 2894 Size: 1657 29 491 79 402 26 156 54 Cuts at: 2894 3018 Size: 124 Fragments arranged by size: 1657 491 402 156 124 79 54 29 26 NarI GG'CG_CC Cuts at: 0 1261 1606 1682 1849 2258 2406 2842 2896 Size: 1261 345 76 167 409 148 436 54 Cuts at: 2896 3018 Size: 122 Fragments arranged by size: 1261 436 409 345 167 148 122 76 54 NcoI C'CATG_G Cuts at: 0 354 3018 Size: 354 2664 NgoMIV G'CCGG_C Cuts at: 0 1655 1684 2175 2254 2656 2682 2838 2892 Size: 1655 29 491 79 402 26 156 54 Cuts at: 2892 3018 Size: 126 Fragments arranged by size: 1655 491 402 156 126 79 54 29 26 NspI r_CATG'y Cuts at: 0 933 3018 Size: 933 2085 PasI CC'CwG_GG Cuts at: 0 2170 3018 Size: 2170 848 PmlI CAC'GTG Cuts at: 0 629 3012 3018 Size: 629 2383 6 Fragments arranged by size: 2383 629 6 PpuMI rG'GwC_Cy Cuts at: 0 2164 2601 2738 3018 Size: 2164 437 137 280 Fragments arranged by size: 2164 437 280 137 PspOMI G'GGCC_C Cuts at: 0 311 345 1428 1444 1565 1594 1715 1917 Size: 311 34 1083 16 121 29 121 202 Cuts at: 1917 3018 Size: 1101 Fragments arranged by size: 1101 1083 311 202 121 121 34 29 16 PspXI vC'TCGA_Gb Cuts at: 0 2485 3018 Size: 2485 533 PstI C_TGCA'G Cuts at: 0 770 1664 3018 Size: 770 894 1354 Fragments arranged by size: 1354 894 770 PvuII CAG'CTG Cuts at: 0 1055 3018 Size: 1055 1963 SacII CC_GC'GG Cuts at: 0 1247 2308 2369 2404 2694 3018 Size: 1247 1061 61 35 290 324 Fragments arranged by size: 1247 1061 324 290 61 35 SanDI GG'GwC_CC Cuts at: 0 2164 2738 3018 Size: 2164 574 280 Fragments arranged by size: 2164 574 280 SapI GCTCTTCn'nnn_ Cuts at: 0 233 1711 3018 Size: 233 1478 1307 Fragments arranged by size: 1478 1307 233 SbfI CC_TGCA'GG Cuts at: 0 770 3018 Size: 770 2248 ScaI AGT'ACT Cuts at: 0 2512 3018 Size: 2512 506 SfcI C'TryA_G Cuts at: 0 766 1089 1660 3018 Size: 766 323 571 1358 Fragments arranged by size: 1358 766 571 323 SfiI GGCCn_nnn'nGGCC Cuts at: 0 679 3018 Size: 679 2339 SfoI GGC'GCC Cuts at: 0 1262 1607 1683 1850 2259 2407 2843 2897 Size: 1262 345 76 167 409 148 436 54 Cuts at: 2897 3018 Size: 121 Fragments arranged by size: 1262 436 409 345 167 148 121 76 54 SgrAI Cr'CCGG_yG Cuts at: 0 1684 2430 2838 2892 3018 Size: 1684 746 408 54 126 Fragments arranged by size: 1684 746 408 126 54 SmaI CCC'GGG Cuts at: 0 1712 1916 2006 2162 2663 2736 2752 3018 Size: 1712 204 90 156 501 73 16 266 Fragments arranged by size: 1712 501 266 204 156 90 73 16 SmlI C'TyrA_G Cuts at: 0 1015 2485 3018 Size: 1015 1470 533 Fragments arranged by size: 1470 1015 533 StuI AGG'CCT Cuts at: 0 436 2059 2491 3018 Size: 436 1623 432 527 Fragments arranged by size: 1623 527 436 432 StyI C'CwwG_G Cuts at: 0 194 354 992 1133 1721 1844 3018 Size: 194 160 638 141 588 123 1174 Fragments arranged by size: 1174 638 588 194 160 141 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 73 1181 3018 Size: 73 1108 1837 Fragments arranged by size: 1837 1108 73 TatI w'GTAC_w Cuts at: 0 2510 3018 Size: 2510 508 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 635 667 3018 Size: 635 32 2351 Fragments arranged by size: 2351 635 32 Tth111I GACn'n_nGTC Cuts at: 0 1467 3018 Size: 1467 1551 XhoI C'TCGA_G Cuts at: 0 2485 3018 Size: 2485 533 XmaI C'CCGG_G Cuts at: 0 1710 1914 2004 2160 2661 2734 2750 3018 Size: 1710 204 90 156 501 73 16 268 Fragments arranged by size: 1710 501 268 204 156 90 73 16 ZraI GAC'GTC Cuts at: 0 717 3018 Size: 717 2301 Enzymes that do cut: AarI AatII Acc65I AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI AscI AvaI BaeI BanI BanII BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PmlI PpuMI PspOMI PspXI PstI PvuII SacII SanDI SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XhoI XmaI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AhdI AloI ApoI AseI AsiSI AvrII BamHI BcgI BciVI BfrBI BmgBI BmtI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstZ17I Bsu36I ClaI DraI EcoICRI EcoRI EcoRV FalI HindIII MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacI SalI SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XmnI