################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 37 GTAGAC GT^[AC][GT]AC AccII 1212 CGCG CG^CG AfaI 218 GTAC GT^AC Aor51HI 855 AGCGCT AGC^GCT BanI 1081 GGTGCC G^G[CT][GA]CC BbrPI 111 CACGTG CAC^GTG BbvCI 897 CCTCAGC CCTCAGC(-5/-2) BcgI 853 CGAGCGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 546 TGATCA T^GATCA BglI 1255 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1455 CTGGAG CTGGAG(16/14) BsaAI 111 CACGTG [CT]AC^GT[GA] BsiEI 966 CGACCG CG[GA][CT]^CG BsmI 1432 GAATGC GAATGC(1/-1) BspHI 836 TCATGA T^CATGA BspLU11I 176 ACATGT A^CATGT BssSI 1384 CTCGTG CACGAG(-5/-1) BstUI 1212 CGCG CG^CG BstXI 522 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 1358 CCGTGG C^C[GA][CT]GG Eco47III 855 AGCGCT AGC^GCT EcoRI 160 GAATTC G^AATTC EcoT14I 182 CCAAGG C^C[AT][AT]GG FauI 1329 GCGGG CCCGC(4/6) FbaI 546 TGATCA T^GATCA FseI 1064 GGCCGGCC GGCCGG^CC HaeII 855 AGCGCT [GA]GCGC^[CT] MslI 753 CATCGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 924 CAGCGG C[AC]G^C[GT]G MvnI 1212 CGCG CG^CG NspI 176 ACATGT [GA]CATG^[CT] PflMI 1197 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 111 CACGTG CAC^GTG PmlI 111 CACGTG CAC^GTG RcaI 836 TCATGA T^CATGA RsaI 218 GTAC GT^AC SacI 1458 GAGCTC GAGCT^C SbfI 1494 CCTGCAGG CCTGCA^GG SmlI 1444 CTCAAG C^T[CT][GA]AG Sse8387I 1494 CCTGCAGG CCTGCA^GG StyI 182 CCAAGG C^C[AT][AT]GG TfiI 585 GAATC G^A[AT]TC Van91I 1197 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 160 GAATTC [GA]^AATT[CT] 1505 AAATTT AflIII 110 ACACGT A^C[GA][CT]GT 176 ACATGT AlwNI 281 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 1198 CAGATCCTG ApoI 160 GAATTC [GA]^AATT[CT] 1505 AAATTT AspI 399 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 685 GACTACGTC BanII 610 GGGCTC G[GA]GC[CT]^C 1458 GAGCTC BbsI 1414 GAAGAC GAAGAC(2/6) 1291 GTCTTC BpuAI 1414 GAAGAC GAAGAC(2/6) 1291 GTCTTC BsgI 172 CTGCAC GTGCAG(16/14) 646 CTGCAC BsmAI 749 GAGAC GTCTC(1/5) 453 GTCTC BstAPI 432 GCAAATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 783 GCACATCCTGC BstYI 282 AGATCC [GA]^GATC[CT] 1199 AGATCC EarI 372 CTCTTC CTCTTC(1/4) 660 GAAGAG EcoNI 15 CCTTTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1265 CCTGCAGCAGG Ksp632I 372 CTCTTC CTCTTC(1/4) 660 GAAGAG MflI 282 AGATCC [GA]^GATC[CT] 1199 AGATCC MseI 326 TTAA T^TAA 1034 TTAA NaeI 481 GCCGGC GCC^GGC 1065 GCCGGC NgoMIV 481 GCCGGC G^CCGGC 1065 GCCGGC PflFI 399 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 685 GACTACGTC PleI 1477 GACTC GAGTC(4/5) 1372 GAGTC SfaNI 1227 GATGC GCATC(5/9) 1345 GCATC TaqI 243 TCGA T^CGA 747 TCGA Tru9I 326 TTAA T^TAA 1034 TTAA Tth111I 399 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 685 GACTACGTC TthHB8I 243 TCGA T^CGA 747 TCGA XhoII 282 AGATCC [GA]^GATC[CT] 1199 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcyI AluI AlwI AspHI AvaII BalI BbvI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI PstI Sau3AI Sau96I ScrFI SfcI StuI TseI Tsp45I Tsp509I TspRI