(Circular) (Six-base) MAPSORT of: pFN21AE1155.seq Check: 7849 from: 1 to: 6223 pFN21AE1155 6223 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2043 3566 1937 Size: 106 1523 4594 Fragments arranged by size: 4594 1523 106 Acc65I G'GTAC_C Cuts at: 3544 3544 Size: 6223 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2268 2364 4286 5670 5938 1632 Size: 636 96 1922 1384 268 1917 Fragments arranged by size: 1922 1917 1384 636 268 96 AfeI AGC'GCT Cuts at: 2862 2862 Size: 6223 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 2115 2181 5122 6099 1203 Size: 912 66 2941 977 1327 Fragments arranged by size: 2941 1327 977 912 66 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2912 1184 Size: 1728 4495 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2765 2797 2765 Size: 32 6191 AlwNI CAG_nnn'CTG Cuts at: 2291 3208 3966 5538 2291 Size: 917 758 1572 2976 Fragments arranged by size: 2976 1572 917 758 ApaLI G'TGCA_C Cuts at: 4280 5436 4280 Size: 1156 5067 ApoI r'AATT_y Cuts at: 1493 2165 3510 3532 3758 3910 6019 1493 Size: 672 1345 22 226 152 2109 1697 Fragments arranged by size: 2109 1697 1345 672 226 152 22 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 3548 1870 Size: 73 1605 4545 Fragments arranged by size: 4545 1605 73 BamHI G'GATC_C Cuts at: 3553 3920 3553 Size: 367 5856 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3086 3544 4999 618 Size: 325 217 627 1299 458 1455 1842 Fragments arranged by size: 1842 1455 1299 627 458 325 217 BanII G_rGCy'C Cuts at: 1391 2619 3467 3542 1391 Size: 1228 848 75 4072 Fragments arranged by size: 4072 1228 848 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3289 3426 928 Size: 971 1390 137 3725 Fragments arranged by size: 3725 1390 971 137 BbvCI CC'TCA_GC Cuts at: 2903 2903 Size: 6223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2847 2881 4414 4448 1903 Size: 34 910 34 1533 34 3678 Fragments arranged by size: 3678 1533 910 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4115 5325 4115 Size: 1210 5013 BclI T'GATC_A Cuts at: 1548 2551 1548 Size: 1003 5220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3266 4834 136 Size: 107 122 71 631 605 1594 1568 1525 Fragments arranged by size: 1594 1568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6218 6218 Size: 6223 BlpI GC'TnA_GC Cuts at: 3640 3640 Size: 6223 Bme1580I G_kGCm'C Cuts at: 1790 4284 5440 1790 Size: 2494 1156 2573 Fragments arranged by size: 2573 2494 1156 BmgBI CAC'GTC Cuts at: 1318 2587 3424 1318 Size: 1269 837 4117 Fragments arranged by size: 4117 1269 837 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2421 3954 4912 Size: 416 53 83 399 24 1004 1533 958 Cuts at: 4912 442 Size: 1753 Fragments arranged by size: 1753 1533 1004 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3481 4883 1239 Size: 2242 1402 2579 Fragments arranged by size: 2579 2242 1402 Bpu10I CC'TnA_GC Cuts at: 2903 2903 Size: 6223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3434 4348 5213 5511 3434 Size: 914 865 298 4146 Fragments arranged by size: 4146 914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4886 882 Size: 617 3387 2219 Fragments arranged by size: 3387 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2118 4014 493 Size: 711 914 1896 2702 Fragments arranged by size: 2702 1896 914 711 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2484 3146 3213 4030 Size: 53 83 186 936 951 662 67 817 Cuts at: 4030 4412 5047 275 Size: 382 635 1451 Fragments arranged by size: 1451 951 936 817 662 635 382 186 83 67 53 BsaWI w'CCGG_w Cuts at: 4656 5054 5328 5475 5886 4656 Size: 398 274 147 411 4993 Fragments arranged by size: 4993 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2883 2913 1178 Size: 30 165 30 1480 30 4488 Fragments arranged by size: 4488 1480 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2891 3175 3389 3457 1186 Size: 979 726 284 214 68 3952 Fragments arranged by size: 3952 979 726 284 214 68 BseYI C'CCAG_C Cuts at: 5426 5922 5426 Size: 496 5727 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2636 1163 Size: 573 426 474 4750 Fragments arranged by size: 4750 573 474 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2974 3673 4434 5462 1540 Size: 275 186 973 699 761 1028 2301 Fragments arranged by size: 2301 1028 973 761 699 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 2133 2990 3467 4284 4369 5440 2108 Size: 25 857 477 817 85 1071 2891 Fragments arranged by size: 2891 1071 857 817 477 85 25 BsmI GAATG_Cn' Cuts at: 3443 3734 3827 3443 Size: 291 93 5839 Fragments arranged by size: 5839 291 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2133 2619 2990 3467 3542 4284 Size: 399 318 25 486 371 477 75 742 Cuts at: 4284 4369 5440 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 477 399 371 318 85 75 25 BspEI T'CCGG_A Cuts at: 5886 5886 Size: 6223 BspHI T'CATG_A Cuts at: 2841 4112 5842 2841 Size: 1271 1730 3222 Fragments arranged by size: 3222 1730 1271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2658 3283 3507 3578 844 Size: 1814 625 224 71 3489 Fragments arranged by size: 3489 1814 625 224 71 BsrBI CCG'CTC Cuts at: 1394 1823 4110 1394 Size: 429 2287 3507 Fragments arranged by size: 3507 2287 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4718 4892 65 Size: 1183 3470 174 1396 Fragments arranged by size: 3470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2486 2958 2973 3070 4867 5054 1700 Size: 110 676 472 15 97 1797 187 2869 Fragments arranged by size: 2869 1797 676 472 187 110 97 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 3389 4283 5295 3389 Size: 894 1012 4317 Fragments arranged by size: 4317 1012 894 BstAPI GCAn_nnn'nTGC Cuts at: 794 2443 2794 794 Size: 1649 351 4223 Fragments arranged by size: 4223 1649 351 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6223 BstXI CCAn_nnnn'nTGG Cuts at: 2534 2534 Size: 6223 BstYI r'GATC_y Cuts at: 1972 2287 3204 3553 3920 4305 4322 5774 5860 Size: 315 917 349 367 385 17 1452 86 Cuts at: 5860 5872 6218 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 917 385 367 349 346 315 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3363 513 Size: 552 939 1359 3373 Fragments arranged by size: 3373 1359 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5484 Fragments arranged by size: 5484 683 56 BtsI GCAGTG_nn' Cuts at: 2916 3364 3739 4533 4553 2916 Size: 448 375 794 20 4586 Fragments arranged by size: 4586 794 448 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 3527 3527 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5230 809 Size: 507 3914 1802 Fragments arranged by size: 3914 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 2956 3057 Size: 54 1425 50 275 18 513 613 101 Cuts at: 3057 3222 3670 4559 6188 8 Size: 165 448 889 1629 43 Fragments arranged by size: 1629 1425 889 613 513 448 275 165 101 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2383 2660 4153 1353 Size: 1030 277 1493 3423 Fragments arranged by size: 3423 1493 1030 277 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2621 2714 3152 3262 4810 5184 Size: 1064 138 1032 93 438 110 1548 374 Cuts at: 5184 5330 387 Size: 146 1280 Fragments arranged by size: 1548 1280 1064 1032 438 374 146 138 110 93 EcoICRI GAG'CTC Cuts at: 3465 3465 Size: 6223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2268 2364 3481 4286 4883 5670 5938 Size: 393 636 96 1117 805 597 787 268 Cuts at: 5938 1239 Size: 1524 Fragments arranged by size: 1524 1117 805 787 636 597 393 268 96 EcoNI CCTnn'n_nnAGG Cuts at: 2024 3274 2024 Size: 1250 4973 EcoO109I rG'GnC_Cy Cuts at: 2017 2307 2523 2017 Size: 290 216 5717 Fragments arranged by size: 5717 290 216 EcoRI G'AATT_C Cuts at: 2165 3532 3910 2165 Size: 1367 378 4478 Fragments arranged by size: 4478 1367 378 FseI GG_CCGG'CC Cuts at: 3074 3074 Size: 6223 FspI TGC'GCA Cuts at: 4729 4729 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 2864 5370 1678 Size: 1186 2506 2531 Fragments arranged by size: 2531 2506 1186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2474 2506 2706 Size: 32 135 32 683 32 187 32 200 Cuts at: 2706 2738 3335 3367 4864 4896 4938 4970 1373 Size: 32 597 32 1497 32 42 32 2626 Fragments arranged by size: 2626 1497 683 597 200 187 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3567 3813 669 Size: 988 281 1629 246 3079 Fragments arranged by size: 3079 1629 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6223 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KpnI G_GTAC'C Cuts at: 3548 3548 Size: 6223 MfeI C'AATT_G Cuts at: 3822 3822 Size: 6223 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2539 5337 5521 1305 Size: 1234 2798 184 2007 Fragments arranged by size: 2798 2007 1234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2345 3059 3224 10 Size: 54 1425 343 513 714 165 3009 Fragments arranged by size: 3009 1425 714 513 343 165 54 MslI CAynn'nnrTG Cuts at: 518 1166 2762 4181 4540 4699 518 Size: 648 1596 1419 359 159 2042 Fragments arranged by size: 2042 1596 1419 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2931 3639 4316 5464 5709 1606 Size: 1325 708 677 1148 245 2120 Fragments arranged by size: 2120 1325 1148 708 677 245 NaeI GCC'GGC Cuts at: 2488 3072 2488 Size: 584 5639 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4732 Fragments arranged by size: 4732 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6223 NgoMIV G'CCGG_C Cuts at: 2486 3070 2486 Size: 584 5639 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6223 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 2185 3581 5126 1132 Size: 97 956 1396 1545 2229 Fragments arranged by size: 2229 1545 1396 956 97 PasI CC'CwG_GG Cuts at: 1348 3076 1348 Size: 1728 4495 PciI A'CATG_T Cuts at: 2181 5122 2181 Size: 2941 3282 PflMI CCAn_nnn'nTGG Cuts at: 1108 3208 1108 Size: 2100 4123 PfoI T'CCnGG_A Cuts at: 2290 2290 Size: 6223 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PmlI CAC'GTG Cuts at: 2118 2118 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3221 3253 4292 4324 5805 5837 3221 Size: 32 1039 32 1481 32 3607 Fragments arranged by size: 3607 1481 1039 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2017 2307 2523 2017 Size: 290 216 5717 Fragments arranged by size: 5717 290 216 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PstI C_TGCA'G Cuts at: 830 2930 3275 3359 3504 3575 830 Size: 2100 345 84 145 71 3478 Fragments arranged by size: 3478 2100 345 145 84 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 SacI G_AGCT'C Cuts at: 3467 3467 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SanDI GG'GwC_CC Cuts at: 2523 2523 Size: 6223 SbfI CC_TGCA'GG Cuts at: 3504 3575 3504 Size: 71 6152 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SfcI C'TryA_G Cuts at: 826 1045 2926 3271 3355 3500 3571 4706 5387 Size: 219 1881 345 84 145 71 1135 681 Cuts at: 5387 5578 826 Size: 191 1471 Fragments arranged by size: 1881 1471 1135 681 345 219 191 145 84 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 3550 1872 Size: 1678 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3449 4327 5228 5490 820 Size: 197 926 1506 878 901 262 1553 Fragments arranged by size: 1553 1506 926 901 878 262 197 SnaBI TAC'GTA Cuts at: 493 4014 493 Size: 3521 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 3026 3134 3269 3477 1517 Size: 1509 108 135 208 4263 Fragments arranged by size: 4263 1509 208 135 108 StyI C'CwwG_G Cuts at: 513 1065 2004 2187 3662 513 Size: 552 939 183 1475 3074 Fragments arranged by size: 3074 1475 939 552 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2564 3074 4250 4267 4420 4605 5050 Size: 1255 984 510 1176 17 153 185 445 Cuts at: 5050 325 Size: 1498 Fragments arranged by size: 1498 1255 1176 984 510 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4469 6181 96 Size: 274 80 33 51 1443 2492 1712 138 Fragments arranged by size: 2492 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6183 6215 6183 Size: 32 6191 Tth111I GACn'n_nGTC Cuts at: 1316 2407 2693 1316 Size: 1091 286 4846 Fragments arranged by size: 4846 1091 286 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6223 XmaI C'CCGG_G Cuts at: 1870 3548 1870 Size: 1678 4545 XmnI GAAnn'nnTTC Cuts at: 4352 4352 Size: 6223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI