Restriction map for
pFN21AE1155
(Linear) (Six-base) MAPSORT of: KIEE1155.seq Check: 3045 from: 1 to: 1518
KIEE1155.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 38 1518
Size: 38 1480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 359 1518
Size: 263 96 1159
Fragments arranged by size:
1159 263 96
AfeI AGC'GCT
Cuts at: 0 857 1518
Size: 857 661
AflIII A'CryG_T
Cuts at: 0 110 176 1518
Size: 110 66 1342
Fragments arranged by size:
1342 110 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 907 1518
Size: 907 611
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 760 792 1518
Size: 760 32 726
Fragments arranged by size:
760 726 32
AlwNI CAG_nnn'CTG
Cuts at: 0 286 1203 1518
Size: 286 917 315
Fragments arranged by size:
917 315 286
ApoI r'AATT_y
Cuts at: 0 160 1505 1518
Size: 160 1345 13
Fragments arranged by size:
1345 160 13
BanI G'GyrC_C
Cuts at: 0 1081 1518
Size: 1081 437
BanII G_rGCy'C
Cuts at: 0 614 1462 1518
Size: 614 848 56
Fragments arranged by size:
848 614 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 1284 1421 1518
Size: 1284 137 97
Fragments arranged by size:
1284 137 97
BbvCI CC'TCA_GC
Cuts at: 0 898 1518
Size: 898 620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 842 876 1518
Size: 842 34 642
Fragments arranged by size:
842 642 34
BclI T'GATC_A
Cuts at: 0 546 1518
Size: 546 972
BglI GCCn_nnn'nGGC
Cuts at: 0 1261 1518
Size: 1261 257
BmgBI CAC'GTC
Cuts at: 0 582 1419 1518
Size: 582 837 99
Fragments arranged by size:
837 582 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 416 1518
Size: 416 1102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1476 1518
Size: 1476 42
Bpu10I CC'TnA_GC
Cuts at: 0 898 1518
Size: 898 620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1518
Size: 1429 89
BsaAI yAC'GTr
Cuts at: 0 113 1518
Size: 113 1405
BsaHI Gr'CG_yC
Cuts at: 0 479 1141 1208 1518
Size: 479 662 67 310
Fragments arranged by size:
662 479 310 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 878 908 1518
Size: 878 30 610
Fragments arranged by size:
878 610 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 886 1170 1384 1452 1518
Size: 160 726 284 214 68 66
Fragments arranged by size:
726 284 214 160 68 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 631 1518
Size: 157 474 887
Fragments arranged by size:
887 474 157
BsiEI CG_ry'CG
Cuts at: 0 969 1518
Size: 969 549
BsiHKAI G_wGCw'C
Cuts at: 0 103 128 985 1462 1518
Size: 103 25 857 477 56
Fragments arranged by size:
857 477 103 56 25
BsmI GAATG_Cn'
Cuts at: 0 1438 1518
Size: 1438 80
Bsp1286I G_dGCh'C
Cuts at: 0 103 128 614 985 1462 1518
Size: 103 25 486 371 477 56
Fragments arranged by size:
486 477 371 103 56 25
BspHI T'CATG_A
Cuts at: 0 836 1518
Size: 836 682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 653 1278 1502 1518
Size: 653 625 224 16
Fragments arranged by size:
653 625 224 16
BsrFI r'CCGG_y
Cuts at: 0 481 953 968 1065 1518
Size: 481 472 15 97 453
Fragments arranged by size:
481 472 453 97 15
BssSI C'ACGA_G
Cuts at: 0 1384 1518
Size: 1384 134
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 789 1518
Size: 438 351 729
Fragments arranged by size:
729 438 351
BstXI CCAn_nnnn'nTGG
Cuts at: 0 529 1518
Size: 529 989
BstYI r'GATC_y
Cuts at: 0 282 1199 1518
Size: 282 917 319
Fragments arranged by size:
917 319 282
BtgI C'CryG_G
Cuts at: 0 1358 1518
Size: 1358 160
BtsI GCAGTG_nn'
Cuts at: 0 911 1359 1518
Size: 911 448 159
Fragments arranged by size:
911 448 159
EaeI y'GGCC_r
Cuts at: 0 338 951 1052 1217 1518
Size: 338 613 101 165 301
Fragments arranged by size:
613 338 301 165 101
EarI CTCTTCn'nnn_
Cuts at: 0 378 655 1518
Size: 378 277 863
Fragments arranged by size:
863 378 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 616 709 1147 1257 1518
Size: 616 93 438 110 261
Fragments arranged by size:
616 438 261 110 93
EcoICRI GAG'CTC
Cuts at: 0 1460 1518
Size: 1460 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 359 1476 1518
Size: 263 96 1117 42
Fragments arranged by size:
1117 263 96 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 1269 1518
Size: 19 1250 249
Fragments arranged by size:
1250 249 19
EcoO109I rG'GnC_Cy
Cuts at: 0 12 302 518 1518
Size: 12 290 216 1000
Fragments arranged by size:
1000 290 216 12
EcoRI G'AATT_C
Cuts at: 0 160 1518
Size: 160 1358
FseI GG_CCGG'CC
Cuts at: 0 1069 1518
Size: 1069 449
HaeII r_GCGC'y
Cuts at: 0 859 1518
Size: 859 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 469 501 701 733 1330 1362
Size: 250 32 187 32 200 32 597 32
Cuts at: 1362 1518
Size: 156
Fragments arranged by size:
597 250 200 187 156 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1518
Size: 534 984
MscI TGG'CCA
Cuts at: 0 340 1054 1219 1518
Size: 340 714 165 299
Fragments arranged by size:
714 340 299 165
MslI CAynn'nnrTG
Cuts at: 0 757 1518
Size: 757 761
MspA1I CmG'CkG
Cuts at: 0 926 1518
Size: 926 592
NaeI GCC'GGC
Cuts at: 0 483 1067 1518
Size: 483 584 451
Fragments arranged by size:
584 483 451
NgoMIV G'CCGG_C
Cuts at: 0 481 1065 1518
Size: 481 584 453
Fragments arranged by size:
584 481 453
NspI r_CATG'y
Cuts at: 0 180 1518
Size: 180 1338
PasI CC'CwG_GG
Cuts at: 0 1071 1518
Size: 1071 447
PciI A'CATG_T
Cuts at: 0 176 1518
Size: 176 1342
PflMI CCAn_nnn'nTGG
Cuts at: 0 1203 1518
Size: 1203 315
PfoI T'CCnGG_A
Cuts at: 0 285 1518
Size: 285 1233
PmlI CAC'GTG
Cuts at: 0 113 1518
Size: 113 1405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1518
Size: 1216 32 270
Fragments arranged by size:
1216 270 32
PpuMI rG'GwC_Cy
Cuts at: 0 12 302 518 1518
Size: 12 290 216 1000
Fragments arranged by size:
1000 290 216 12
PstI C_TGCA'G
Cuts at: 0 925 1270 1354 1499 1518
Size: 925 345 84 145 19
Fragments arranged by size:
925 345 145 84 19
SacI G_AGCT'C
Cuts at: 0 1462 1518
Size: 1462 56
SanDI GG'GwC_CC
Cuts at: 0 518 1518
Size: 518 1000
SbfI CC_TGCA'GG
Cuts at: 0 1499 1518
Size: 1499 19
SfcI C'TryA_G
Cuts at: 0 921 1266 1350 1495 1518
Size: 921 345 84 145 23
Fragments arranged by size:
921 345 145 84 23
SmlI C'TyrA_G
Cuts at: 0 1444 1518
Size: 1444 74
StuI AGG'CCT
Cuts at: 0 1021 1129 1264 1472 1518
Size: 1021 108 135 208 46
Fragments arranged by size:
1021 208 135 108 46
StyI C'CwwG_G
Cuts at: 0 182 1518
Size: 182 1336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 1069 1518
Size: 559 510 449
Fragments arranged by size:
559 510 449
Tth111I GACn'n_nGTC
Cuts at: 0 402 688 1518
Size: 402 286 830
Fragments arranged by size:
830 402 286
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AloI AlwNI ApoI
BanI BanII BbsI BbvCI BcgI BclI BglI BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PstI
SacI SanDI SbfI SfcI SmlI StuI StyI TaqII
Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BciVI BfrBI BglII BlpI Bme1580I BmtI
BplI BsaI BsaBI BsaWI BseYI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRV
FalI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI XbaI XcmI XhoI
XmaI XmnI ZraI