Restriction map for
pFN21AE2509
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccII AccIII AclI AcyI
AgeI Alw44I AocI ApaI ApaLI
AscI AseI AsnI AspI AvrII
BamHI BbeI BbvCI BcgI BciVI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
Hin1I HpaI KasI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 818 GGTACC G^GTACC
AccI 275 GTCTAC GT^[AC][GT]AC
AflII 193 CTTAAG C^TTAAG
AflIII 661 ACACGT A^C[GA][CT]GT
AhdI 838 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2115 AGCGCT AGC^GCT
Asp718I 818 GGTACC G^GTACC
AspEI 838 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 528 GAGCTC G[AT]GC[AT]^C
AvaI 1667 CCCGAG C^[CT]CG[GA]G
AviII 1782 TGCGCA TGC^GCA
BalI 1574 TGGCCA TGG^CCA
BanI 818 GGTACC G^G[CT][GA]CC
BanII 528 GAGCTC G[GA]GC[CT]^C
BbrPI 662 CACGTG CAC^GTG
BfrI 193 CTTAAG C^TTAAG
BglII 1194 AGATCT A^GATCT
BmyI 528 GAGCTC G[AGT]GC[ACT]^C
BsgI 1939 CTGCAC GTGCAG(16/14)
BsiHKAI 528 GAGCTC G[AT]GC[AT]^C
BsoBI 1667 CCCGAG C^[CT]CG[GA]G
Bsp1286I 528 GAGCTC G[AGT]GC[ACT]^C
BspMI 1965 GCAGGT ACCTGC(4/8)
DraI 145 TTTAAA TTT^AAA
EaeI 1574 TGGCCA [CT]^GGCC[GA]
Eam1105I 838 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1413 GAAGAG CTCTTC(1/4)
Eco47III 2115 AGCGCT AGC^GCT
EcoNI 1977 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1782 TGCGCA TGC^GCA
HincII 556 GTTGAC GT[CT]^[GA]AC
HindII 556 GTTGAC GT[CT]^[GA]AC
HindIII 1183 AAGCTT A^AGCTT
KpnI 818 GGTACC GGTAC^C
Ksp632I 1413 GAAGAG CTCTTC(1/4)
MfeI 1898 CAATTG C^AATTG
MluNI 1574 TGGCCA TGG^CCA
MscI 1574 TGGCCA TGG^CCA
MunI 1898 CAATTG C^AATTG
PmaCI 662 CACGTG CAC^GTG
PmlI 662 CACGTG CAC^GTG
SacI 528 GAGCTC GAGCT^C
ScaI 634 AGTACT AGT^ACT
SmlI 193 CTTAAG C^T[CT][GA]AG
StuI 1048 AGGCCT AGG^CCT
TaqI 1411 TCGA T^CGA
TthHB8I 1411 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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AvaII 1660 GGTCC G^G[AT]CC
1791 GGTCC
BclI 182 TGATCA T^GATCA
666 TGATCA
BsaAI 404 TACGTG [CT]AC^GT[GA]
662 CACGTG
BsmI 32 GCATTC GAATGC(1/-1)
1747 GCATTC
BstXI 117 CCATTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1961 CCATGCAGGTGG
Cfr13I 1660 GGTCC G^G[ACGT]CC
1791 GGTCC
FbaI 182 TGATCA T^GATCA
666 TGATCA
HaeII 48 GGCGCT [GA]GCGC^[CT]
2115 AGCGCT
HaeIII 1049 GGCC GG^CC
1575 GGCC
HgaI 54 GACGC GACGC(5/10)
2231 GCGTC
PflMI 1285 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2248 CCAGAGCCTGG
PstI 1752 CTGCAG CTGCA^G
2074 CTGCAG
Sau96I 1660 GGTCC G^G[ACGT]CC
1791 GGTCC
Van91I 1285 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2248 CCAGAGCCTGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BbsI BbvI
BcnI BfaI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI DdeI DpnII
EcoRII Fnu4HI FokI HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI