(Circular) (Six-base) MAPSORT of: pFN21AE2509.seq Check: 8063 from: 1 to: 6967 pFN21AE2509 6967 bp With 182 enzymes: SgfI * February 18, 2014 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3961 3961 Size: 6967 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4777 278 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2281 4310 1937 Size: 344 2029 4594 Fragments arranged by size: 4594 2029 344 Acc65I G'GTAC_C Cuts at: 2823 4288 2823 Size: 1465 5502 AclI AA'CG_TT Cuts at: 1563 5094 5467 1563 Size: 3531 373 3063 Fragments arranged by size: 3531 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2125 2838 2964 3357 3756 3797 5030 6414 Size: 493 713 126 393 399 41 1233 1384 Cuts at: 6414 6682 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1233 713 493 399 393 268 126 41 AfeI AGC'GCT Cuts at: 4122 4122 Size: 6967 AflII C'TTAA_G Cuts at: 820 1017 2198 820 Size: 197 1181 5589 Fragments arranged by size: 5589 1181 197 AflIII A'CryG_T Cuts at: 1203 2666 5866 6843 1203 Size: 1463 3200 977 1327 Fragments arranged by size: 3200 1463 1327 977 AgeI A'CCGG_T Cuts at: 5798 5798 Size: 6967 AhdI GACnn_n'nnGTC Cuts at: 2848 5696 2848 Size: 2848 4119 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6967 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6967 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 2744 Size: 32 6935 AlwNI CAG_nnn'CTG Cuts at: 3601 4087 4259 4710 6282 3601 Size: 486 172 451 1572 4286 Fragments arranged by size: 4286 1572 486 451 172 ApaLI G'TGCA_C Cuts at: 5024 6180 5024 Size: 1156 5811 ApoI r'AATT_y Cuts at: 1493 2146 2182 2236 2467 3870 4276 4502 4654 Size: 653 36 54 231 1403 406 226 152 Cuts at: 4654 6763 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1403 653 406 231 226 152 54 36 AseI AT'TA_AT Cuts at: 160 1032 5521 160 Size: 872 4489 1606 Fragments arranged by size: 4489 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 AvaI C'yCGr_G Cuts at: 1870 1943 3672 4292 1870 Size: 73 1729 620 4545 Fragments arranged by size: 4545 1729 620 73 BamHI G'GATC_C Cuts at: 4297 4664 4297 Size: 367 6600 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2823 4288 5743 618 Size: 325 217 627 1036 1465 1455 1842 Fragments arranged by size: 1842 1465 1455 1036 627 325 217 BanII G_rGCy'C Cuts at: 1391 2537 4286 1391 Size: 1146 1749 4072 Fragments arranged by size: 4072 1749 1146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2510 2844 3744 4025 4179 928 Size: 971 611 334 900 281 154 3716 Fragments arranged by size: 3716 971 900 611 334 281 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5158 5192 1903 Size: 34 3221 34 3678 Fragments arranged by size: 3678 3221 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4859 6069 4859 Size: 1210 5757 BclI T'GATC_A Cuts at: 1548 2187 2671 1548 Size: 639 484 5844 Fragments arranged by size: 5844 639 484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5578 136 Size: 107 122 71 631 605 3906 1525 Fragments arranged by size: 3906 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3199 6962 3199 Size: 3763 3204 BlpI GC'TnA_GC Cuts at: 4384 4384 Size: 6967 Bme1580I G_kGCm'C Cuts at: 1790 5028 6184 1790 Size: 3238 1156 2573 Fragments arranged by size: 3238 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6967 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2705 4698 5656 Size: 416 53 83 399 24 1288 1993 958 Cuts at: 5656 442 Size: 1753 Fragments arranged by size: 1993 1753 1288 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6967 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3520 3520 Size: 6967 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2405 2850 3151 3623 3863 4034 5627 Size: 845 321 445 301 472 240 171 1593 Cuts at: 5627 1239 Size: 2579 Fragments arranged by size: 2579 1593 845 472 445 321 301 240 171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5092 5957 6255 5092 Size: 865 298 5804 Fragments arranged by size: 5804 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5630 882 Size: 617 4131 2219 Fragments arranged by size: 4131 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2411 2669 4758 493 Size: 711 1207 258 2089 2702 Fragments arranged by size: 2702 2089 1207 711 258 BsaBI GATnn'nnATC Cuts at: 6961 6961 Size: 6967 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4774 5156 5791 275 Size: 53 83 186 936 3241 382 635 1451 Fragments arranged by size: 3241 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5400 5798 6072 6219 6630 5400 Size: 398 274 147 411 5737 Fragments arranged by size: 5737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3149 3179 1178 Size: 30 165 30 1746 30 4966 Fragments arranged by size: 4966 1746 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3330 3632 3635 4183 1186 Size: 2144 302 3 548 3970 Fragments arranged by size: 3970 2144 548 302 3 BseYI C'CCAG_C Cuts at: 3999 6170 6666 3999 Size: 2171 496 4300 Fragments arranged by size: 4300 2171 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3929 1163 Size: 573 2193 4201 Fragments arranged by size: 4201 2193 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4417 5178 6206 1540 Size: 275 186 2416 761 1028 2301 Fragments arranged by size: 2416 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2537 5028 5113 6184 2537 Size: 2491 85 1071 3320 Fragments arranged by size: 3320 2491 1071 85 BsmI GAATG_Cn' Cuts at: 2037 3752 4478 4571 2037 Size: 1715 726 93 4433 Fragments arranged by size: 4433 1715 726 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2537 4286 5028 5113 6184 1391 Size: 399 747 1749 742 85 1071 2174 Fragments arranged by size: 2174 1749 1071 747 742 399 85 BspEI T'CCGG_A Cuts at: 6630 6630 Size: 6967 BspHI T'CATG_A Cuts at: 4856 6586 4856 Size: 1730 5237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3961 4322 844 Size: 3117 361 3489 Fragments arranged by size: 3489 3117 361 BsrBI CCG'CTC Cuts at: 1394 1823 4854 1394 Size: 429 3031 3507 Fragments arranged by size: 3507 3031 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2021 2093 2665 5462 5636 65 Size: 1183 773 72 572 2797 174 1396 Fragments arranged by size: 2797 1396 1183 773 572 174 72 BsrFI r'CCGG_y Cuts at: 1700 1810 5611 5798 1700 Size: 110 3801 187 2869 Fragments arranged by size: 3801 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6967 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6967 BssSI C'ACGA_G Cuts at: 5027 6039 5027 Size: 1012 5955 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6967 BstBI TT'CG_AA Cuts at: 5771 5771 Size: 6967 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6967 BstXI CCAn_nnnn'nTGG Cuts at: 2129 3973 2129 Size: 1844 5123 BstYI r'GATC_y Cuts at: 1972 2102 2958 3199 4049 4297 4664 5049 5066 Size: 130 856 241 850 248 367 385 17 Cuts at: 5066 6518 6604 6616 6962 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 856 850 385 367 346 248 241 130 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5476 Fragments arranged by size: 5476 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4146 487 Size: 683 56 2920 3308 Fragments arranged by size: 3308 2920 683 56 BtsI GCAGTG_nn' Cuts at: 3350 4483 5277 5297 3350 Size: 1133 794 20 5020 Fragments arranged by size: 5020 1133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6967 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6932 DraI TTT'AAA Cuts at: 2152 4271 2152 Size: 2119 4848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5974 809 Size: 507 4658 1802 Fragments arranged by size: 4658 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3579 4414 5303 Size: 54 1425 50 275 18 1749 835 889 Cuts at: 5303 6932 8 Size: 1629 43 Fragments arranged by size: 1749 1629 1425 889 835 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4414 1537 Size: 275 2602 4090 Fragments arranged by size: 4090 2602 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3413 4897 1353 Size: 2060 1484 3423 Fragments arranged by size: 3423 2060 1484 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5554 5928 6074 387 Size: 1064 138 435 3530 374 146 1280 Fragments arranged by size: 3530 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2535 2535 Size: 6967 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2125 2405 2838 2850 2964 3151 Size: 393 452 41 280 433 12 114 187 Cuts at: 3151 3357 3623 3756 3797 3863 4034 5030 5627 Size: 206 266 133 41 66 171 996 597 Cuts at: 5627 6414 6682 1239 Size: 787 268 1524 Fragments arranged by size: 1524 996 787 597 452 433 393 280 268 266 206 187 171 133 114 66 41 41 12 EcoNI CCTnn'n_nnAGG Cuts at: 3986 3986 Size: 6967 EcoRI G'AATT_C Cuts at: 4276 4654 4276 Size: 378 6589 FspI TGC'GCA Cuts at: 3789 5473 3789 Size: 1684 5283 HaeII r_GCGC'y Cuts at: 1678 2057 4124 6114 1678 Size: 379 2067 1990 2531 Fragments arranged by size: 2531 2067 1990 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2308 2340 2834 2866 3325 Size: 32 135 32 736 32 494 32 459 Cuts at: 3325 3357 5608 5640 5682 5714 1373 Size: 32 2251 32 42 32 2626 Fragments arranged by size: 2626 2251 736 494 459 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2563 4311 4557 669 Size: 988 281 625 1748 246 3079 Fragments arranged by size: 3079 1748 988 625 281 246 HindIII A'AGCT_T Cuts at: 748 3188 748 Size: 2440 4527 HpaI GTT'AAC Cuts at: 4557 4557 Size: 6967 KpnI G_GTAC'C Cuts at: 2827 4292 2827 Size: 1465 5502 MfeI C'AATT_G Cuts at: 3903 4566 3903 Size: 663 6304 MluI A'CGCG_T Cuts at: 6843 6843 Size: 6967 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3861 6081 6265 1305 Size: 2556 2220 184 2007 Fragments arranged by size: 2556 2220 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3581 10 Size: 54 1425 343 1749 3396 Fragments arranged by size: 3396 1749 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3129 3624 3930 3971 4925 5284 5443 Size: 648 1963 495 306 41 954 359 159 Cuts at: 5443 518 Size: 2042 Fragments arranged by size: 2042 1963 954 648 495 359 306 159 41 MspA1I CmG'CkG Cuts at: 1606 3793 3961 4003 4087 4383 5060 6208 6453 Size: 2187 168 42 84 296 677 1148 245 Cuts at: 6453 1606 Size: 2120 Fragments arranged by size: 2187 2120 1148 677 296 245 168 84 42 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5476 Fragments arranged by size: 5476 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6967 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6967 NotI GC'GGCC_GC Cuts at: 4414 4414 Size: 6967 NspI r_CATG'y Cuts at: 1132 1229 4325 5870 1132 Size: 97 3096 1545 2229 Fragments arranged by size: 3096 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6967 PciI A'CATG_T Cuts at: 5866 5866 Size: 6967 PflMI CCAn_nnn'nTGG Cuts at: 1108 3296 4259 1108 Size: 2188 963 3816 Fragments arranged by size: 3816 2188 963 PfoI T'CCnGG_A Cuts at: 3600 4112 3600 Size: 512 6455 PmeI GTTT'AAAC Cuts at: 4271 4271 Size: 6967 PmlI CAC'GTG Cuts at: 2669 2669 Size: 6967 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 6549 6581 5036 Size: 32 1481 32 5422 Fragments arranged by size: 5422 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6967 PsiI TTA'TAA Cuts at: 2247 4537 2247 Size: 2290 4677 PstI C_TGCA'G Cuts at: 830 3761 4083 4319 830 Size: 2931 322 236 3478 Fragments arranged by size: 3478 2931 322 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6967 PvuII CAG'CTG Cuts at: 3793 3961 4003 4087 3793 Size: 168 42 84 6673 Fragments arranged by size: 6673 168 84 42 SacI G_AGCT'C Cuts at: 2537 2537 Size: 6967 SalI G'TCGA_C Cuts at: 1936 4309 1936 Size: 2373 4594 SbfI CC_TGCA'GG Cuts at: 4319 4319 Size: 6967 ScaI AGT'ACT Cuts at: 2641 5215 6927 2641 Size: 2574 1712 2681 Fragments arranged by size: 2681 2574 1712 SfcI C'TryA_G Cuts at: 826 1045 3486 3757 4079 4315 5450 6131 6322 Size: 219 2441 271 322 236 1135 681 191 Cuts at: 6322 826 Size: 1471 Fragments arranged by size: 2441 1471 1135 681 322 271 236 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6967 SmaI CCC'GGG Cuts at: 1872 4294 1872 Size: 2422 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2198 5071 5972 6234 820 Size: 197 926 255 2873 901 262 1553 Fragments arranged by size: 2873 1553 926 901 262 255 197 SnaBI TAC'GTA Cuts at: 493 4758 493 Size: 4265 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6967 SphI G_CATG'C Cuts at: 1132 4325 1132 Size: 3193 3774 SspI AAT'ATT Cuts at: 5 52 4891 5 Size: 47 4839 2081 Fragments arranged by size: 4839 2081 47 StuI AGG'CCT Cuts at: 1517 3055 1517 Size: 1538 5429 StyI C'CwwG_G Cuts at: 513 1065 2004 4406 513 Size: 552 939 2402 3074 Fragments arranged by size: 3074 2402 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2415 2933 3653 4994 5011 5164 5349 Size: 1255 835 518 720 1341 17 153 185 Cuts at: 5349 5794 325 Size: 445 1498 Fragments arranged by size: 1498 1341 1255 835 720 518 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2639 3238 5213 Size: 274 80 33 51 1443 662 599 1975 Cuts at: 5213 6925 96 Size: 1712 138 Fragments arranged by size: 1975 1712 1443 662 599 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6927 6959 6927 Size: 32 6935 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6967 XbaI T'CTAG_A Cuts at: 4303 4303 Size: 6967 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6967 XmaI C'CCGG_G Cuts at: 1870 4292 1870 Size: 2422 4545 XmnI GAAnn'nnTTC Cuts at: 2037 2469 3752 3870 5096 2037 Size: 432 1283 118 1226 3908 Fragments arranged by size: 3908 1283 1226 432 118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4775 276 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI