Restriction map for
pFN21AE6623
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstYI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 243 GTATAC GT^[AC][GT]AC
AccII 101 CGCG CG^CG
AocI 543 CCTGAGG CC^T[ACGT]AGG
ApaI 605 GGGCCC GGGCC^C
AspHI 592 GAGCAC G[AT]GC[AT]^C
AvaI 602 CCCGGG C^[CT]CG[GA]G
AvaII 696 GGACC G^G[AT]CC
BanI 96 GGTGCC G^G[CT][GA]CC
BbrPI 473 CACGTG CAC^GTG
BbvCI 609 CCTCAGC CCTCAGC(-5/-2)
BfaI 481 CTAG C^TAG
BsaAI 473 CACGTG [CT]AC^GT[GA]
BsaI 385 GGTCTC GGTCTC(1/5)
BsiHKAI 592 GAGCAC G[AT]GC[AT]^C
BsmFI 695 GGGAC GGGAC(10/14)
BsoBI 602 CCCGGG C^[CT]CG[GA]G
BspLU11I 677 ACATGT A^CATGT
BspMI 864 GCAGGT ACCTGC(4/8)
BsrDI 838 GCAATG GCAATG(2/0)
Bst1107I 243 GTATAC GTA^TAC
BstUI 101 CGCG CG^CG
BstXI 662 CCAGCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 243 GTATAC GTA^TAC
Bsu36I 543 CCTGAGG CC^T[ACGT]AGG
DraII 605 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 30 TGGCCG [CT]^GGCC[GA]
Eco81I 543 CCTGAGG CC^T[ACGT]AGG
EcoO109I 605 GGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 809 GGCGCT [GA]GCGC^[CT]
MaeI 481 CTAG C^TAG
MaeII 474 ACGT A^CGT
MvnI 101 CGCG CG^CG
PflMI 375 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 473 CACGTG CAC^GTG
PmlI 473 CACGTG CAC^GTG
PstI 52 CTGCAG CTGCA^G
SfcI 52 CTGCAG C^T[GA][CT]AG
SmaI 602 CCCGGG CCC^GGG
Van91I 375 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 602 CCCGGG C^CCGGG
XmaI 602 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 502 GTAC GT^AC
720 GTAC
AlwI 214 GATCC GGATC(4/5)
579 GATCC
BanII 371 GAGCCC G[GA]GC[CT]^C
605 GGGCCC
BglI 32 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
733 GCCATGCTGGC
BsgI 395 GTGCAG GTGCAG(16/14)
908 GTGCAG
BsmI 572 GAATGC GAATGC(1/-1)
183 GCATTC
CfoI 25 GCGC GCG^C
810 GCGC
DpnII 214 GATC ^GATC
579 GATC
DsaI 71 CCGTGG C^C[GA][CT]GG
339 CCACGG
EarI 484 GAAGAG CTCTTC(1/4)
522 GAAGAG
HgaI 918 GACGC GACGC(5/10)
948 GCGTC
HhaI 25 GCGC GCG^C
810 GCGC
HinP1I 25 GCGC G^CGC
810 GCGC
Ksp632I 484 GAAGAG CTCTTC(1/4)
522 GAAGAG
MaeIII 448 GTGAC ^GT[ACGT]AC
916 GTGAC
MboI 214 GATC ^GATC
579 GATC
MseI 713 TTAA T^TAA
830 TTAA
MspA1I 55 CAGCTG C[AC]G^C[GT]G
119 CAGCTG
NdeII 214 GATC ^GATC
579 GATC
NspI 407 GCATGT [GA]CATG^[CT]
677 ACATGT
PvuII 55 CAGCTG CAG^CTG
119 CAGCTG
RsaI 502 GTAC GT^AC
720 GTAC
Sau3AI 214 GATC ^GATC
579 GATC
SmlI 322 CTTGAG C^T[CT][GA]AG
922 CTCAAG
Tru9I 713 TTAA T^TAA
830 TTAA
Tsp45I 448 GTGAC ^GT[GC]AC
916 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AflIII AluI AlwNI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MboII MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI TseI TspRI