(Circular) (Six-base) MAPSORT of: pFN21AE6623.seq Check: 9451 from: 1 to: 5659 pFN21AE6623 5659 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2249 3002 1937 Size: 312 753 4594 Fragments arranged by size: 4594 753 312 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2127 3722 5106 5374 1632 Size: 474 21 1595 1384 268 1917 Fragments arranged by size: 1917 1595 1384 474 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 2477 2479 2682 4558 5535 1203 Size: 1274 2 203 1876 977 1327 Fragments arranged by size: 1876 1327 1274 977 203 2 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 2480 1166 Size: 1314 4345 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2642 1184 Size: 1458 4201 AlwNI CAG_nnn'CTG Cuts at: 2386 2503 2548 3402 4974 2386 Size: 117 45 854 1572 3071 Fragments arranged by size: 3071 1572 854 117 45 ApaI G_GGCC'C Cuts at: 2614 2614 Size: 5659 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2968 3194 3346 5455 1493 Size: 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2607 2984 1870 Size: 73 664 377 4545 Fragments arranged by size: 4545 664 377 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 2980 4435 618 Size: 325 217 627 314 879 1455 1842 Fragments arranged by size: 1842 1455 879 627 325 314 217 BanII G_rGCy'C Cuts at: 1391 2380 2614 2978 1391 Size: 989 234 364 4072 Fragments arranged by size: 4072 989 364 234 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2133 2220 2540 928 Size: 971 234 87 320 4047 Fragments arranged by size: 4047 971 320 234 87 BbvCI CC'TCA_GC Cuts at: 2615 2615 Size: 5659 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2043 2744 Size: 107 122 71 631 605 337 34 701 Cuts at: 2744 4270 136 Size: 1526 1525 Fragments arranged by size: 1526 1525 701 631 605 337 122 107 71 34 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2340 2614 3720 4876 1790 Size: 550 274 1106 1156 2573 Fragments arranged by size: 2573 1156 1106 550 274 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 2452 3390 Size: 416 53 83 399 24 957 78 938 Cuts at: 3390 4348 442 Size: 958 1753 Fragments arranged by size: 1753 958 957 938 416 399 83 78 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2380 2778 4319 1239 Size: 829 312 398 1541 2579 Fragments arranged by size: 2579 1541 829 398 312 Bpu10I CC'TnA_GC Cuts at: 2135 2615 2654 2135 Size: 480 39 5140 Fragments arranged by size: 5140 480 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 2912 3784 4649 4947 2348 Size: 564 872 865 298 3060 Fragments arranged by size: 3060 872 865 564 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2396 4322 882 Size: 617 897 1926 2219 Fragments arranged by size: 2219 1926 897 617 BsaAI yAC'GTr Cuts at: 493 1204 2480 3450 493 Size: 711 1276 970 2702 Fragments arranged by size: 2702 1276 970 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5434 Fragments arranged by size: 5434 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2285 2288 2306 2507 2779 1186 Size: 1096 3 3 18 201 272 4066 Fragments arranged by size: 4066 1096 272 201 18 3 3 BseYI C'CCAG_C Cuts at: 2696 4862 5358 2696 Size: 2166 496 2997 Fragments arranged by size: 2997 2166 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2421 2934 1163 Size: 573 685 513 3888 Fragments arranged by size: 3888 685 573 513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 3720 3805 4876 2601 Size: 1119 85 1071 3384 Fragments arranged by size: 3384 1119 1071 85 BsmI GAATG_Cn' Cuts at: 2188 2583 3170 3263 2188 Size: 395 587 93 4584 Fragments arranged by size: 4584 587 395 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2340 2380 2601 2614 2978 3720 3805 Size: 399 550 40 221 13 364 742 85 Cuts at: 3805 4876 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 550 399 364 221 85 40 13 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2860 3014 844 Size: 2016 154 3489 Fragments arranged by size: 3489 2016 154 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2850 4154 4328 65 Size: 1183 1602 1304 174 1396 Fragments arranged by size: 1602 1396 1304 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstXI CCAn_nnnn'nTGG Cuts at: 2674 2674 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654 Size: 1017 367 385 17 1452 86 12 346 Cuts at: 5654 1972 Size: 1977 Fragments arranged by size: 1977 1452 1017 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2250 2250 Size: 5659 Bsu36I CC'TnA_GG Cuts at: 2549 2549 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 2344 513 Size: 552 939 72 268 3828 Fragments arranged by size: 3828 939 552 268 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2080 487 Size: 683 56 854 4066 Fragments arranged by size: 4066 854 683 56 BtsI GCAGTG_nn' Cuts at: 2437 2565 2859 2925 3175 3969 3989 2437 Size: 128 294 66 250 794 20 4107 Fragments arranged by size: 4107 794 294 250 128 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 3106 3995 Size: 54 1425 50 275 18 205 1071 889 Cuts at: 3995 5624 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1071 889 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2484 2522 3589 1353 Size: 1131 38 1067 3423 Fragments arranged by size: 3423 1131 1067 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2537 4246 4620 4766 387 Size: 1064 138 948 1709 374 146 1280 Fragments arranged by size: 1709 1280 1064 948 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2106 2127 2380 2778 3722 4319 Size: 393 436 38 21 253 398 944 597 Cuts at: 4319 5106 5374 1239 Size: 787 268 1524 Fragments arranged by size: 1524 944 787 597 436 398 393 268 253 38 21 EcoO109I rG'GnC_Cy Cuts at: 2611 2611 Size: 5659 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2818 4806 1678 Size: 1140 1988 2531 Fragments arranged by size: 2531 1988 1140 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 4300 4332 4374 Size: 32 135 32 638 32 2058 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 2058 638 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 2624 2669 3617 3976 4135 518 Size: 648 1314 144 45 948 359 159 2042 Fragments arranged by size: 2042 1314 948 648 359 159 144 45 MspA1I CmG'CkG Cuts at: 1606 2062 2126 3075 3752 4900 5145 1606 Size: 456 64 949 677 1148 245 2120 Fragments arranged by size: 2120 1148 949 677 456 245 64 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4168 Fragments arranged by size: 4168 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2416 2686 3017 4562 1132 Size: 97 1187 270 331 1545 2229 Fragments arranged by size: 2229 1545 1187 331 270 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 2682 4558 2682 Size: 1876 3783 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 1108 Size: 1278 4381 PfoI T'CCnGG_A Cuts at: 2820 2820 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PmlI CAC'GTG Cuts at: 2480 2480 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PspOMI G'GGCC_C Cuts at: 2610 2610 Size: 5659 PstI C_TGCA'G Cuts at: 830 2061 3011 830 Size: 1231 950 3478 Fragments arranged by size: 3478 1231 950 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2062 2126 2062 Size: 64 5595 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2057 3007 4142 4823 5014 826 Size: 219 1012 950 1135 681 191 1471 Fragments arranged by size: 1471 1135 1012 950 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2609 2986 1872 Size: 737 377 4545 Fragments arranged by size: 4545 737 377 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 2927 3763 4664 4926 820 Size: 197 926 384 600 836 901 262 1553 Fragments arranged by size: 1553 926 901 836 600 384 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2004 3098 513 Size: 552 939 1094 3074 Fragments arranged by size: 3074 1094 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2900 3686 3703 3856 4041 4486 325 Size: 1255 1320 786 17 153 185 445 1498 Fragments arranged by size: 1498 1320 1255 786 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2669 2701 5619 5651 2669 Size: 32 2918 32 2677 Fragments arranged by size: 2918 2677 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2607 2984 1870 Size: 737 377 4545 Fragments arranged by size: 4545 737 377 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI