Restriction map for
pFN21AE6623
(Linear) (Six-base) MAPSORT of: KIEE6623.seq Check: 3782 from: 1 to: 954
KIEE6623.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AccI GT'mk_AC
Cuts at: 0 244 954
Size: 244 710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 122 954
Size: 101 21 832
Fragments arranged by size:
832 101 21
AflIII A'CryG_T
Cuts at: 0 472 474 677 954
Size: 472 2 203 277
Fragments arranged by size:
472 277 203 2
AleI CACnn'nnGTG
Cuts at: 0 475 954
Size: 475 479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 637 954
Size: 637 317
AlwNI CAG_nnn'CTG
Cuts at: 0 381 498 543 954
Size: 381 117 45 411
Fragments arranged by size:
411 381 117 45
ApaI G_GGCC'C
Cuts at: 0 609 954
Size: 609 345
AvaI C'yCGr_G
Cuts at: 0 602 954
Size: 602 352
BanI G'GyrC_C
Cuts at: 0 96 954
Size: 96 858
BanII G_rGCy'C
Cuts at: 0 375 609 954
Size: 375 234 345
Fragments arranged by size:
375 345 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 128 215 535 954
Size: 128 87 320 419
Fragments arranged by size:
419 320 128 87
BbvCI CC'TCA_GC
Cuts at: 0 610 954
Size: 610 344
BglI GCCn_nnn'nGGC
Cuts at: 0 38 739 954
Size: 38 701 215
Fragments arranged by size:
701 215 38
Bme1580I G_kGCm'C
Cuts at: 0 335 609 954
Size: 335 274 345
Fragments arranged by size:
345 335 274
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 447 954
Size: 369 78 507
Fragments arranged by size:
507 369 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 375 773 954
Size: 63 312 398 181
Fragments arranged by size:
398 312 181 63
Bpu10I CC'TnA_GC
Cuts at: 0 130 610 649 954
Size: 130 480 39 305
Fragments arranged by size:
480 305 130 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 907 954
Size: 343 564 47
Fragments arranged by size:
564 343 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 954
Size: 391 563
BsaAI yAC'GTr
Cuts at: 0 475 954
Size: 475 479
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 280 283 301 502 774 954
Size: 277 3 3 18 201 272 180
Fragments arranged by size:
277 272 201 180 18 3 3
BseYI C'CCAG_C
Cuts at: 0 691 954
Size: 691 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 929 954
Size: 416 513 25
Fragments arranged by size:
513 416 25
BsiHKAI G_wGCw'C
Cuts at: 0 596 954
Size: 596 358
BsmI GAATG_Cn'
Cuts at: 0 183 578 954
Size: 183 395 376
Fragments arranged by size:
395 376 183
Bsp1286I G_dGCh'C
Cuts at: 0 335 375 596 609 954
Size: 335 40 221 13 345
Fragments arranged by size:
345 335 221 40 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 855 954
Size: 855 99
BsrDI GCAATG_nn'
Cuts at: 0 845 954
Size: 845 109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 669 954
Size: 669 285
BstZ17I GTA'TAC
Cuts at: 0 245 954
Size: 245 709
Bsu36I CC'TnA_GG
Cuts at: 0 544 954
Size: 544 410
BtgI C'CryG_G
Cuts at: 0 71 339 954
Size: 71 268 615
Fragments arranged by size:
615 268 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 75 954
Size: 75 879
BtsI GCAGTG_nn'
Cuts at: 0 432 560 854 920 954
Size: 432 128 294 66 34
Fragments arranged by size:
432 294 128 66 34
EaeI y'GGCC_r
Cuts at: 0 30 954
Size: 30 924
EarI CTCTTCn'nnn_
Cuts at: 0 479 517 954
Size: 479 38 437
Fragments arranged by size:
479 437 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 954
Size: 532 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 101 122 375 773 954
Size: 63 38 21 253 398 181
Fragments arranged by size:
398 253 181 63 38 21
EcoO109I rG'GnC_Cy
Cuts at: 0 606 954
Size: 606 348
HaeII r_GCGC'y
Cuts at: 0 813 954
Size: 813 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 954
Size: 205 32 717
Fragments arranged by size:
717 205 32
MslI CAynn'nnrTG
Cuts at: 0 475 619 664 954
Size: 475 144 45 290
Fragments arranged by size:
475 290 144 45
MspA1I CmG'CkG
Cuts at: 0 57 121 954
Size: 57 64 833
Fragments arranged by size:
833 64 57
NspI r_CATG'y
Cuts at: 0 411 681 954
Size: 411 270 273
Fragments arranged by size:
411 273 270
PciI A'CATG_T
Cuts at: 0 677 954
Size: 677 277
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 954
Size: 381 573
PfoI T'CCnGG_A
Cuts at: 0 815 954
Size: 815 139
PmlI CAC'GTG
Cuts at: 0 475 954
Size: 475 479
PspOMI G'GGCC_C
Cuts at: 0 605 954
Size: 605 349
PstI C_TGCA'G
Cuts at: 0 56 954
Size: 56 898
PvuII CAG'CTG
Cuts at: 0 57 121 954
Size: 57 64 833
Fragments arranged by size:
833 64 57
SfcI C'TryA_G
Cuts at: 0 52 954
Size: 52 902
SmaI CCC'GGG
Cuts at: 0 604 954
Size: 604 350
SmlI C'TyrA_G
Cuts at: 0 322 922 954
Size: 322 600 32
Fragments arranged by size:
600 322 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 895 954
Size: 895 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 664 696 954
Size: 664 32 258
Fragments arranged by size:
664 258 32
XmaI C'CCGG_G
Cuts at: 0 602 954
Size: 602 352
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BanI BanII BbsI BbvCI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I HaeII
Hin4I MslI MspA1I NspI PciI PflMI PfoI PmlI
PspOMI PstI PvuII SfcI SmaI SmlI TaqII TstI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI ZraI