################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AclI AflII AhdI AseI AsnI AspEI AvrII BbrPI BciVI BfrI BlnI BsiWI BspDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI Eam1105I EcoO65I EcoRV EcoT22I HpaI KspI MluI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 979 GACGTC GACGT^C Alw44I 2074 GTGCAC G^TGCAC AocI 4143 CCTAAGG CC^T[ACGT]AGG ApaLI 2074 GTGCAC G^TGCAC AscI 5585 GGCGCGCC GG^CGCGCC AspI 25 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 5973 TGGCCA TGG^CCA BbvCI 834 GCTGAGG CCTCAGC(-5/-2) BglII 5231 AGATCT A^GATCT BsaAI 4533 TACGTG [CT]AC^GT[GA] BsaBI 2962 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 2411 ACATGT A^CATGT Bsu36I 4143 CCTAAGG CC^T[ACGT]AGG Eco81I 4143 CCTAAGG CC^T[ACGT]AGG EcoRI 4149 GAATTC G^AATTC FseI 5950 GGCCGGCC GGCCGG^CC MamI 2962 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4211 CAATTG C^AATTG MluNI 5973 TGGCCA TGG^CCA MscI 5973 TGGCCA TGG^CCA MunI 4211 CAATTG C^AATTG NdeI 301 CATATG CA^TATG NheI 903 GCTAGC G^CTAGC PflFI 25 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC SalI 2638 GTCGAC G^TCGAC SexAI 3790 ACCTGGT A^CC[AT]GGT SphI 3903 GCATGC GCATG^C SspI 3526 AATATT AAT^ATT Tth111I 25 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2269 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 2423 GGTACC G^GTACC 4196 GGTACC AflIII 1688 ACGTGT A^C[GA][CT]GT 2411 ACATGT AgeI 218 ACCGGT A^CCGGT 734 ACCGGT Asp700I 2950 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4395 GAAGGCCTTC Asp718I 2423 GGTACC G^GTACC 4196 GGTACC AviII 4911 TGCGCA TGC^GCA 5285 TGCGCA BbeI 3068 GGCGCC GGCGC^C 4436 GGCGCC BcgI 3555 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3750 CGACCAGAGTGC BlpI 795 GCTGAGC GC^T[ACGT]AGC 5826 GCTGAGC Bpu1102I 795 GCTGAGC GC^T[ACGT]AGC 5826 GCTGAGC Bsp1407I 406 TGTACA T^GTACA 2867 TGTACA BspHI 4049 TCATGA T^CATGA 6029 TCATGA BsrDI 3831 CATTGC GCAATG(2/0) 3839 GCAATG BsrGI 406 TGTACA T^GTACA 2867 TGTACA BssHII 5401 GCGCGC G^CGCGC 5586 GCGCGC BssSI 6295 CACGAG CACGAG(-5/-1) 2649 CTCGTG BstXI 64 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5501 CCAGACCCCTGG CelII 795 GCTGAGC GC^T[ACGT]AGC 5826 GCTGAGC DraI 1015 TTTAAA TTT^AAA 4099 TTTAAA DraIII 1953 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 5443 CACTCCGTG DrdI 1045 GACGAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3471 GACATTTCTGTC FspI 4911 TGCGCA TGC^GCA 5285 TGCGCA KasI 3068 GGCGCC G^GCGCC 4436 GGCGCC KpnI 2423 GGTACC GGTAC^C 4196 GGTACC NarI 3068 GGCGCC GG^CGCC 4436 GGCGCC NspI 2411 ACATGT [GA]CATG^[CT] 3903 GCATGC PinAI 218 ACCGGT A^CCGGT 734 ACCGGT RcaI 4049 TCATGA T^CATGA 6029 TCATGA SfoI 3068 GGCGCC GGC^GCC 4436 GGCGCC SmaI 162 CCCGGG CCC^GGG 5570 CCCGGG SmlI 997 CTCAAG C^T[CT][GA]AG 3379 CTCAAG SspBI 406 TGTACA T^GTACA 2867 TGTACA XmaCI 162 CCCGGG C^CCGGG 5570 CCCGGG XmaI 162 CCCGGG C^CCGGG 5570 CCCGGG XmnI 2950 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4395 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI ApoI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII