################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI ApaI ApoI AscI Asp700I Asp718I AspI AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglI BlnI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrFI BssHII BstBI Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfiI SfuI SgrAI SpeI SphI Sse8387I StuI SwaI Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 2521 GACGTC GACGT^C Alw44I 2044 GTGCAC G^TGCAC ApaLI 2044 GTGCAC G^TGCAC AseI 444 ATTAAT AT^TAAT AsnI 444 ATTAAT AT^TAAT AviII 2590 TGCGCA TGC^GCA BalI 117 TGGCCA TGG^CCA BbeI 2205 GGCGCC GGCGC^C BbsI 511 GTCTTC GAAGAC(2/6) BbvCI 1263 GCTGAGG CCTCAGC(-5/-2) BfaI 1451 CTAG C^TAG BglII 2643 AGATCT A^GATCT BlpI 1669 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1669 GCTGAGC GC^T[ACGT]AGC BpuAI 511 GTCTTC GAAGAC(2/6) BsaWI 2657 TCCGGT [AT]^CCGG[AT] Bsp1407I 413 TGTACA T^GTACA BsrBI 1885 GAGCGG CCGCTC(-3/-3) BsrDI 1908 CATTGC GCAATG(2/0) BsrGI 413 TGTACA T^GTACA Bst1107I 633 GTATAC GTA^TAC BstEII 639 GGTCACC G^GT[ACGT]ACC BstPI 639 GGTCACC G^GT[ACGT]ACC BstYI 2643 AGATCT [GA]^GATC[CT] BstZ17I 633 GTATAC GTA^TAC CelII 1669 GCTGAGC GC^T[ACGT]AGC DraIII 2038 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 639 GGTCACC G^GT[ACGT]ACC EcoT22I 646 ATGCAT ATGCA^T FspI 2590 TGCGCA TGC^GCA KasI 2205 GGCGCC G^GCGCC KspI 1725 CCGCGG CCGC^GG MaeI 1451 CTAG C^TAG MflI 2643 AGATCT [GA]^GATC[CT] MluNI 117 TGGCCA TGG^CCA MscI 117 TGGCCA TGG^CCA MseI 445 TTAA T^TAA NarI 2205 GGCGCC GG^CGCC NcoI 710 CCATGG C^CATGG NheI 1450 GCTAGC G^CTAGC NsiI 646 ATGCAT ATGCA^T PaeR7I 890 CTCGAG C^TCGAG PshBI 444 ATTAAT AT^TAAT SacII 1725 CCGCGG CCGC^GG ScaI 2783 AGTACT AGT^ACT SexAI 2411 ACCTGGT A^CC[AT]GGT SfoI 2205 GGCGCC GGC^GCC SmlI 890 CTCGAG C^T[CT][GA]AG SnaBI 1945 TACGTA TAC^GTA SspBI 413 TGTACA T^GTACA SspI 2068 AATATT AAT^ATT TfiI 1482 GATTC G^A[AT]TC Tru9I 445 TTAA T^TAA XhoI 890 CTCGAG C^TCGAG XhoII 2643 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 633 GTATAC GT^[AC][GT]AC 838 GTCTAC AhdI 222 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 553 GACCCTGAGTC AocI 944 CCTCAGG CC^T[ACGT]AGG 1184 CCTCAGG Aor51HI 89 AGCGCT AGC^GCT 2248 AGCGCT AspEI 222 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 553 GACCCTGAGTC BsaAI 1945 TACGTA [CT]AC^GT[GA] 2287 TACGTG BstAPI 1552 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2220 GCAGCTCCTGC Bsu36I 944 CCTCAGG CC^T[ACGT]AGG 1184 CCTCAGG Eam1105I 222 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 553 GACCCTGAGTC Eco47III 89 AGCGCT AGC^GCT 2248 AGCGCT Eco81I 944 CCTCAGG CC^T[ACGT]AGG 1184 CCTCAGG EcoNI 556 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 940 CCTGCCTCAGG SacI 1918 GAGCTC GAGCT^C 2728 GAGCTC SmaI 953 CCCGGG CCC^GGG 2405 CCCGGG XmaCI 953 CCCGGG C^CCGGG 2405 CCCGGG XmaI 953 CCCGGG C^CCGGG 2405 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI