Restriction map for
pFN21AE3335
(Circular) (Six-base) MAPSORT of: pFN21AE3335.seq Check: 5936 from: 1 to: 7627
pFN21AE3335 7627 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3508 3562 4038 3508
Size: 54 476 7097
Fragments arranged by size:
7097 476 54
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4530 5437 278
Size: 53 83 186 936 2994 907 2468
Fragments arranged by size:
2994 2468 936 907 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2639 2844 4970 1937
Size: 702 205 2126 4594
Fragments arranged by size:
4594 2126 702 205
Acc65I G'GTAC_C
Cuts at: 4948 4948
Size: 7627
AclI AA'CG_TT
Cuts at: 1563 5754 6127 1563
Size: 4191 373 3063
Fragments arranged by size:
4191 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 3614 3690 4082 4153 5690 7074 7342
Size: 716 1266 76 392 71 1537 1384 268
Cuts at: 7342 1632
Size: 1917
Fragments arranged by size:
1917 1537 1384 1266 716 392 268 76
71
AfeI AGC'GCT
Cuts at: 2096 4255 2096
Size: 2159 5468
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7430
AflIII A'CryG_T
Cuts at: 1203 6526 7503 1203
Size: 5323 977 1327
Fragments arranged by size:
5323 1327 977
AgeI A'CCGG_T
Cuts at: 6458 6458
Size: 7627
AhdI GACnn_n'nnGTC
Cuts at: 2232 2563 6356 2232
Size: 331 3793 3503
Fragments arranged by size:
3793 3503 331
AleI CACnn'nnGTG
Cuts at: 1166 3068 3689 1166
Size: 1902 621 5104
Fragments arranged by size:
5104 1902 621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4133 4163 1184
Size: 2949 30 4648
Fragments arranged by size:
4648 2949 30
AlwNI CAG_nnn'CTG
Cuts at: 2821 3220 3592 3785 4009 4105 4231 4474 4536
Size: 399 372 193 224 96 126 243 62
Cuts at: 4536 5370 6942 2821
Size: 834 1572 3506
Fragments arranged by size:
3506 1572 834 399 372 243 224 193
126 96 62
ApaLI G'TGCA_C
Cuts at: 4049 5684 6840 4049
Size: 1635 1156 4836
Fragments arranged by size:
4836 1635 1156
ApoI r'AATT_y
Cuts at: 1493 4936 5162 5314 7423 1493
Size: 3443 226 152 2109 1697
Fragments arranged by size:
3443 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2450 6181 160
Size: 872 1418 3731 1606
Fragments arranged by size:
3731 1606 1418 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 2895 2958 3598 3989 4214 4410
Size: 73 279 673 63 640 391 225 196
Cuts at: 4410 4952 1870
Size: 542 4545
Fragments arranged by size:
4545 673 640 542 391 279 225 196
73 63
BamHI G'GATC_C
Cuts at: 4957 5324 4957
Size: 367 7260
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2853 3079 3550 3858
Size: 325 217 627 321 745 226 471 308
Cuts at: 3858 4210 4948 6403 618
Size: 352 738 1455 1842
Fragments arranged by size:
1842 1455 745 738 627 471 352 325
321 308 226 217
BanII G_rGCy'C
Cuts at: 1391 2196 2668 3164 3927 3990 4363 4439 4566
Size: 805 472 496 763 63 373 76 127
Cuts at: 4566 4737 4946 1391
Size: 171 209 4072
Fragments arranged by size:
4072 805 763 496 472 373 209 171
127 76 63
BbeI G_GCGC'C
Cuts at: 4214 4214
Size: 7627
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 928
Size: 971 610 6046
Fragments arranged by size:
6046 971 610
BbvCI CC'TCA_GC
Cuts at: 3269 3269
Size: 7627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5818 5852 1903
Size: 34 3881 34 3678
Fragments arranged by size:
3881 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5519 6729 5519
Size: 1210 6417
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7627
BfrBI ATG'CAT
Cuts at: 2653 2653
Size: 7627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6238 136
Size: 107 122 71 631 605 4566 1525
Fragments arranged by size:
4566 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4648 7622 4648
Size: 2974 4653
BlpI GC'TnA_GC
Cuts at: 3675 5044 3675
Size: 1369 6258
Bme1580I G_kGCm'C
Cuts at: 1790 4053 4579 5688 6844 1790
Size: 2263 526 1109 1156 2573
Fragments arranged by size:
2573 2263 1156 1109 526
BmgBI CAC'GTC
Cuts at: 1318 3721 1318
Size: 2403 5224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3242 3580 3895
Size: 416 53 83 399 24 1825 338 315
Cuts at: 3895 4733 5358 6316 442
Size: 838 625 958 1753
Fragments arranged by size:
1825 1753 958 838 625 416 399 338
315 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3459 1056
Size: 2403 5224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 4136 4645 4712 2620
Size: 1516 509 67 5535
Fragments arranged by size:
5535 1516 509 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2017 2180 2186 3300 3396 3941 4004 4400
Size: 778 163 6 1114 96 545 63 396
Cuts at: 4400 4496 4562 4601 6287 1239
Size: 96 66 39 1686 2579
Fragments arranged by size:
2579 1686 1114 778 545 396 163 96
96 66 63 39 6
Bpu10I CC'TnA_GC
Cuts at: 2475 3269 3362 2475
Size: 794 93 6740
Fragments arranged by size:
6740 794 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5752 6617 6915 5752
Size: 865 298 6464
Fragments arranged by size:
6464 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 3533 4683 6290 882
Size: 617 1068 966 1150 1607 2219
Fragments arranged by size:
2219 1607 1150 1068 966 617
BsaAI yAC'GTr
Cuts at: 493 1204 3952 4294 5418 493
Size: 711 2748 342 1124 2702
Fragments arranged by size:
2748 2702 1124 711 342
BsaBI GATnn'nnATC
Cuts at: 7621 7621
Size: 7627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2753 2841 4211 4527
Size: 53 83 186 936 1220 88 1370 316
Cuts at: 4527 5434 5816 6451 275
Size: 907 382 635 1451
Fragments arranged by size:
1451 1370 1220 936 907 635 382 316
186 88 83 53
BsaWI w'CCGG_w
Cuts at: 4662 6060 6458 6732 6879 7290 4662
Size: 1398 398 274 147 411 4999
Fragments arranged by size:
4999 1398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3298 3328 4345 4375 4535
Size: 30 165 30 1895 30 1017 30 160
Cuts at: 4535 4565 4800 4830 1178
Size: 30 235 30 3975
Fragments arranged by size:
3975 1895 1017 235 165 160 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3628 3788 4808 4855 1186
Size: 2442 160 1020 47 3958
Fragments arranged by size:
3958 2442 1020 160 47
BseYI C'CCAG_C
Cuts at: 2038 2052 2188 2401 2668 2925 2994 3167 3334
Size: 14 136 213 267 257 69 173 167
Cuts at: 3334 3430 3651 3724 6830 7326 2038
Size: 96 221 73 3106 496 2339
Fragments arranged by size:
3106 2339 496 267 257 221 213 173
167 136 96 73 69 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2152 3239 3578 3868 4016 1163
Size: 573 416 1087 339 290 148 4774
Fragments arranged by size:
4774 1087 573 416 339 290 148
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5077 5838 6866 1540
Size: 275 186 3076 761 1028 2301
Fragments arranged by size:
3076 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3873 3927 4053 4122 4626 4737 5688 5773 6844
Size: 54 126 69 504 111 951 85 1071
Cuts at: 6844 3873
Size: 4656
Fragments arranged by size:
4656 1071 951 504 126 111 85 69
54
BsmI GAATG_Cn'
Cuts at: 5138 5231 5138
Size: 93 7534
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2668 3164 3873 3927 3990 4053
Size: 399 406 472 496 709 54 63 63
Cuts at: 4053 4122 4363 4439 4566 4579 4626 4737 4946
Size: 69 241 76 127 13 47 111 209
Cuts at: 4946 5688 5773 6844 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 709 496 472 406 399
241 209 127 111 85 76 69 63
63 54 47 13
BspEI T'CCGG_A
Cuts at: 7290 7290
Size: 7627
BspHI T'CATG_A
Cuts at: 5516 7246 5516
Size: 1730 5897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3453 3508 3562 4038 4701 4982 844
Size: 2609 55 54 476 663 281 3489
Fragments arranged by size:
3489 2609 663 476 281 55 54
BsrBI CCG'CTC
Cuts at: 1394 1823 3892 5514 1394
Size: 429 2069 1622 3507
Fragments arranged by size:
3507 2069 1622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3912 6122 6296 65
Size: 1183 2664 2210 174 1396
Fragments arranged by size:
2664 2210 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6271 6458 1700
Size: 110 4461 187 2869
Fragments arranged by size:
4461 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 96
Size: 2322 5305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7627
BssSI C'ACGA_G
Cuts at: 3084 3236 4619 5687 6699 3084
Size: 152 1383 1068 1012 4012
Fragments arranged by size:
4012 1383 1068 1012 152
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3563 4231 794
Size: 2769 668 4190
Fragments arranged by size:
4190 2769 668
BstBI TT'CG_AA
Cuts at: 6431 6431
Size: 7627
BstEII G'GTnAC_C
Cuts at: 1184 2644 1184
Size: 1460 6167
BstXI CCAn_nnnn'nTGG
Cuts at: 2120 3216 4258 4319 2120
Size: 1096 1042 61 5428
Fragments arranged by size:
5428 1096 1042 61
BstYI r'GATC_y
Cuts at: 1972 4648 4957 5324 5709 5726 7178 7264 7276
Size: 2676 309 367 385 17 1452 86 12
Cuts at: 7276 7622 1972
Size: 346 1977
Fragments arranged by size:
2676 1977 1452 385 367 346 309 86
17 12
BstZ17I GTA'TAC
Cuts at: 2640 2640
Size: 7627
Bsu36I CC'TnA_GG
Cuts at: 2950 3190 2950
Size: 240 7387
BtgI C'CryG_G
Cuts at: 513 1065 2177 2715 3730 4808 513
Size: 552 1112 538 1015 1078 3332
Fragments arranged by size:
3332 1112 1078 1015 552 538
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6888
Fragments arranged by size:
6888 683 56
BtsI GCAGTG_nn'
Cuts at: 3171 3202 3462 3786 4439 4605 5143 5937 5957
Size: 31 260 324 653 166 538 794 20
Cuts at: 5957 3171
Size: 4841
Fragments arranged by size:
4841 794 653 538 324 260 166 31
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7592
DraI TTT'AAA
Cuts at: 4931 4931
Size: 7627
DraIII CAC_nnn'GTG
Cuts at: 4048 4048
Size: 7627
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6634 809
Size: 507 5318 1802
Fragments arranged by size:
5318 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2626 4156
Size: 54 1425 50 275 18 292 504 1530
Cuts at: 4156 4762 5074 5963 7592 8
Size: 606 312 889 1629 43
Fragments arranged by size:
1629 1530 1425 889 606 504 312 292
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5074 1537
Size: 275 3262 4090
Fragments arranged by size:
4090 3262 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2981 3617 4380 4868 5557 1353
Size: 1628 636 763 488 689 3423
Fragments arranged by size:
3423 1628 763 689 636 488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2078 6214 6588 6734 387
Size: 1064 138 489 4136 374 146 1280
Fragments arranged by size:
4136 1280 1064 489 374 146 138
EcoICRI GAG'CTC
Cuts at: 3925 4735 3925
Size: 810 6817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2180 2186 2348 3300 3396 3614
Size: 393 385 163 6 162 952 96 218
Cuts at: 3614 3690 3941 4004 4082 4153 4400 4496 4562
Size: 76 251 63 78 71 247 96 66
Cuts at: 4562 4601 5690 6287 7074 7342 1239
Size: 39 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 952 787 597 393 385 268
251 247 218 163 162 96 96 78
76 71 66 63 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2565 2949 2565
Size: 384 7243
EcoO109I rG'GnC_Cy
Cuts at: 2117 2323 2430 2600 2818 2954 3331 3434 3896
Size: 206 107 170 218 136 377 103 462
Cuts at: 3896 4057 4102 4414 2117
Size: 161 45 312 5330
Fragments arranged by size:
5330 462 377 312 218 206 170 161
136 107 103 45
EcoRI G'AATT_C
Cuts at: 4936 5314 4936
Size: 378 7249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 7627
FspI TGC'GCA
Cuts at: 4597 6133 4597
Size: 1536 6091
HaeII r_GCGC'y
Cuts at: 1678 2098 2148 2216 2711 4214 4257 6774 1678
Size: 420 50 68 495 1503 43 2517 2531
Fragments arranged by size:
2531 2517 1503 495 420 68 50 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2218 2250 2332 2364 2549
Size: 32 135 32 646 32 82 32 185
Cuts at: 2549 2581 3262 3294 3298 3330 3572 3604 4253
Size: 32 681 32 4 32 242 32 649
Cuts at: 4253 4285 4535 4567 4743 4775 6268 6300 6342
Size: 32 250 32 176 32 1493 32 42
Cuts at: 6342 6374 1373
Size: 32 2626
Fragments arranged by size:
2626 1493 681 649 646 250 242 185
176 135 82 42 32 32 32 32
32 32 32 32 32 32 32 32
32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4971 5217 669
Size: 988 281 3033 246 3079
Fragments arranged by size:
3079 3033 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7627
HpaI GTT'AAC
Cuts at: 5217 5217
Size: 7627
KasI G'GCGC_C
Cuts at: 4210 4210
Size: 7627
KpnI G_GTAC'C
Cuts at: 4952 4952
Size: 7627
MfeI C'AATT_G
Cuts at: 5226 5226
Size: 7627
MluI A'CGCG_T
Cuts at: 7503 7503
Size: 7627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2962 4402 6741 6925 1305
Size: 1657 1440 2339 184 2007
Fragments arranged by size:
2339 2007 1657 1440 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2124 10
Size: 54 1425 343 292 5513
Fragments arranged by size:
5513 1425 343 292 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3068 3263 3689 4631 5585 5944
Size: 648 1215 687 195 426 942 954 359
Cuts at: 5944 6103 518
Size: 159 2042
Fragments arranged by size:
2042 1215 954 942 687 648 426 359
195 159
MspA1I CmG'CkG
Cuts at: 1606 2136 2875 2925 2998 3732 4009 4161 4639
Size: 530 739 50 73 734 277 152 478
Cuts at: 4639 5043 5720 6868 7113 1606
Size: 404 677 1148 245 2120
Fragments arranged by size:
2120 1148 739 734 677 530 478 404
277 245 152 73 50
NarI GG'CG_CC
Cuts at: 4211 4211
Size: 7627
NcoI C'CATG_G
Cuts at: 513 1065 2715 513
Size: 552 1650 5425
Fragments arranged by size:
5425 1650 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7627
NheI G'CTAG_C
Cuts at: 1052 3455 1052
Size: 2403 5224
NotI GC'GGCC_GC
Cuts at: 5074 5074
Size: 7627
NsiI A_TGCA'T
Cuts at: 2655 2655
Size: 7627
NspI r_CATG'y
Cuts at: 1132 1229 4985 6530 1132
Size: 97 3756 1545 2229
Fragments arranged by size:
3756 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2113 3286 4910 1348
Size: 765 1173 1624 4065
Fragments arranged by size:
4065 1624 1173 765
PciI A'CATG_T
Cuts at: 6526 6526
Size: 7627
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2408 4105 4474 4910 1108
Size: 1300 1697 369 436 3825
Fragments arranged by size:
3825 1697 1300 436 369
PfoI T'CCnGG_A
Cuts at: 4521 4605 4521
Size: 84 7543
PmeI GTTT'AAAC
Cuts at: 4931 4931
Size: 7627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5696 5728 7209 7241 5696
Size: 32 1481 32 6082
Fragments arranged by size:
6082 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2117 2323 2430 2600 2818 3434 3896 4057 4414
Size: 206 107 170 218 616 462 161 357
Cuts at: 4414 2117
Size: 5330
Fragments arranged by size:
5330 616 462 357 218 206 170 161
107
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7627
PsiI TTA'TAA
Cuts at: 5197 5197
Size: 7627
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2623 2655 2675 2707 2623
Size: 32 20 32 7543
Fragments arranged by size:
7543 32 32 20
PstI C_TGCA'G
Cuts at: 830 3304 3559 3846 4005 4227 4276 4644 4979
Size: 2474 255 287 159 222 49 368 335
Cuts at: 4979 830
Size: 3478
Fragments arranged by size:
3478 2474 368 335 287 255 222 159
49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7627
PvuII CAG'CTG
Cuts at: 2136 2875 2998 4009 2136
Size: 739 123 1011 5754
Fragments arranged by size:
5754 1011 739 123
SacI G_AGCT'C
Cuts at: 3927 4737 3927
Size: 810 6817
SacII CC_GC'GG
Cuts at: 3733 3733
Size: 7627
SalI G'TCGA_C
Cuts at: 1936 4969 1936
Size: 3033 4594
SanDI GG'GwC_CC
Cuts at: 2430 2600 3434 3896 2430
Size: 170 834 462 6161
Fragments arranged by size:
6161 834 462 170
SbfI CC_TGCA'GG
Cuts at: 4979 4979
Size: 7627
ScaI AGT'ACT
Cuts at: 4790 5875 7587 4790
Size: 1085 1712 4830
Fragments arranged by size:
4830 1712 1085
SexAI A'CCwGG_T
Cuts at: 4416 4416
Size: 7627
SfcI C'TryA_G
Cuts at: 826 1045 3300 3555 3842 4001 4162 4223 4272
Size: 219 2255 255 287 159 161 61 49
Cuts at: 4272 4640 4726 4975 6110 6791 6982 826
Size: 368 86 249 1135 681 191 1471
Fragments arranged by size:
2255 1471 1135 681 368 287 255 249
219 191 161 159 86 61 49
SfoI GGC'GCC
Cuts at: 4212 4212
Size: 7627
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7627
SmaI CCC'GGG
Cuts at: 1872 2960 4412 4954 1872
Size: 1088 1452 542 4545
Fragments arranged by size:
4545 1452 1088 542
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2895 5731 6632 6894 820
Size: 197 926 952 2836 901 262 1553
Fragments arranged by size:
2836 1553 952 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3952 5418 493
Size: 3459 1466 2702
Fragments arranged by size:
3459 2702 1466
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7627
SphI G_CATG'C
Cuts at: 1132 4985 1132
Size: 3853 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5551 5
Size: 47 4023 1476 2081
Fragments arranged by size:
4023 2081 1476 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7627
StyI C'CwwG_G
Cuts at: 513 1065 2101 2283 2715 2985 3714 5066 513
Size: 552 1036 182 432 270 729 1352 3074
Fragments arranged by size:
3074 1352 1036 729 552 432 270 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 3008 3181 5654 5671 5824 6009
Size: 1255 546 882 173 2473 17 153 185
Cuts at: 6009 6454 325
Size: 445 1498
Fragments arranged by size:
2473 1498 1255 882 546 445 185 173
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 4788 5873
Size: 274 80 33 51 1443 441 2370 1085
Cuts at: 5873 7585 96
Size: 1712 138
Fragments arranged by size:
2370 1712 1443 1085 441 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2584 2616 2738 2770 3765 3797 4345
Size: 32 237 32 122 32 995 32 548
Cuts at: 4345 4377 4800 4832 7587 7619 2315
Size: 32 423 32 2755 32 2323
Fragments arranged by size:
2755 2323 995 548 423 237 122 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7627
XbaI T'CTAG_A
Cuts at: 4963 4963
Size: 7627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2676 3721 4772 2476
Size: 200 1045 1051 5331
Fragments arranged by size:
5331 1051 1045 200
XhoI C'TCGA_G
Cuts at: 1943 2895 1943
Size: 952 6675
XmaI C'CCGG_G
Cuts at: 1870 2958 4410 4952 1870
Size: 1088 1452 542 4545
Fragments arranged by size:
4545 1452 1088 542
XmnI GAAnn'nnTTC
Cuts at: 5756 5756
Size: 7627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4528 5435 276
Size: 53 83 186 936 2994 907 2468
Fragments arranged by size:
2994 2468 936 907 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BsiWI BsmBI EcoRV
FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI
PspXI RsrII SapI SfiI SrfI SwaI