Restriction map for
pFN21AE3680
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 235 GTCTAC GT^[AC][GT]AC
AlwNI 77 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 466 GGCACC G^G[CT][GA]CC
BanII 455 GAGCTC G[GA]GC[CT]^C
BbvCI 41 CCTCAGC CCTCAGC(-5/-2)
BpmI 107 CTCCAG CTGGAG(16/14)
BsaAI 238 TACGTG [CT]AC^GT[GA]
BsmAI 73 GAGAC GTCTC(1/5)
BsmFI 443 GTCCC GGGAC(10/14)
BsrFI 331 GCCGGT [GA]^CCGG[CT]
BsrI 31 ACTGG ACTGG(1/-1)
CfoI 196 GCGC GCG^C
Cfr10I 331 GCCGGT [GA]^CCGG[CT]
DdeI 42 CTCAG C^T[ACGT]AG
DpnII 306 GATC ^GATC
DrdI 342 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 26 CGGCCA [CT]^GGCC[GA]
EarI 70 GAAGAG CTCTTC(1/4)
HaeII 195 AGCGCC [GA]GCGC^[CT]
HapII 332 CCGG C^CGG
HhaI 196 GCGC GCG^C
HinP1I 196 GCGC G^CGC
HincII 85 GTTAAC GT[CT]^[GA]AC
HindII 85 GTTAAC GT[CT]^[GA]AC
HpaI 85 GTTAAC GTT^AAC
HpaII 332 CCGG C^CGG
HphI 254 TCACC GGTGA(8/7)
Ksp632I 70 GAAGAG CTCTTC(1/4)
MaeIII 142 GTGAC ^GT[ACGT]AC
MboI 306 GATC ^GATC
MboII 70 GAAGA GAAGA(8/7)
MseI 86 TTAA T^TAA
MspA1I 316 CAGCTG C[AC]G^C[GT]G
MspI 332 CCGG C^CGG
NdeII 306 GATC ^GATC
PleI 312 GAGTC GAGTC(4/5)
PpuMI 12 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 316 CAGCTG CAG^CTG
SacI 455 GAGCTC GAGCT^C
Sau3AI 306 GATC ^GATC
SbfI 420 CCTGCAGG CCTGCA^GG
SfaNI 415 GATGC GCATC(5/9)
SgrAI 330 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 420 CCTGCAGG CCTGCA^GG
TfiI 168 GAATC G^A[AT]TC
Tru9I 86 TTAA T^TAA
Tsp45I 142 GTGAC ^GT[GC]AC
Tsp509I 219 AATT ^AATT
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# TWO CUT ENZYMES ( 15 enzymes) #
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AspHI 335 GTGCTC G[AT]GC[AT]^C
455 GAGCTC
BsaJI 121 CCTGGG C^C[ACGT][ACGT]GG
462 CCTGGG
BsgI 226 GTGCAG GTGCAG(16/14)
241 GTGCAG
BsiHKAI 335 GTGCTC G[AT]GC[AT]^C
455 GAGCTC
DraII 12 AGGACCT [GA]G^G[ACGT]CC[CT]
147 GGGGCCT
EcoO109I 12 AGGACCT [GA]G^G[ACGT]CC[CT]
147 GGGGCCT
FauI 356 GCGGG CCCGC(4/6)
385 GCGGG
HinfI 168 GAATC G^A[ACGT]TC
312 GAGTC
MaeII 239 ACGT A^CGT
428 ACGT
MwoI 46 GCACCATCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
380 GCATAGCGGGC
NlaIII 117 CATG CATG^
165 CATG
PstI 421 CTGCAG CTGCA^G
450 CTGCAG
SfcI 421 CTGCAG C^T[GA][CT]AG
450 CTGCAG
TspRI 30 CACTG CA[GC]TG[ACGT][ACGT]^
77 CAGTG
XcmI 21 CCAGGCGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
251 CCATCACCTTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I EcoRII Fnu4HI
FokI HaeIII ItaI MnlI MvaI
NlaIV Sau96I ScrFI TseI