################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacII SalI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwNI 349 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 435 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 316 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 279 GGATCC G^GATCC BanI 242 GGTGCC G^G[CT][GA]CC BcnI 267 CCCGG CC^[GC]GG BsgI 421 GTGCAG GTGCAG(16/14) BsmAI 166 GAGAC GTCTC(1/5) BsmI 50 GAATGC GAATGC(1/-1) BspHI 323 TCATGA T^CATGA BsrI 489 CCAGT ACTGG(1/-1) BstAPI 348 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 270 GGATG GGATG(2/0) Cac8I 423 GCAGGC GC[ACGT]^[ACGT]GC DsaI 505 CCACGG C^C[GA][CT]GG EarI 472 CTCTTC CTCTTC(1/4) FauI 283 CCCGC CCCGC(4/6) FokI 270 GGATG GGATG(9/13) HapII 268 CCGG C^CGG HinfI 97 GAATC G^A[ACGT]TC HpaII 268 CCGG C^CGG Ksp632I 472 CTCTTC CTCTTC(1/4) MaeII 105 ACGT A^CGT MaeIII 314 GTGAC ^GT[ACGT]AC MspA1I 349 CAGCGG C[AC]G^C[GT]G MspI 268 CCGG C^CGG NciI 267 CCCGG CC^[GC]GG PflFI 316 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 323 TCATGA T^CATGA SapI 471 GCTCTTC GCTCTTC(1/4) SexAI 361 ACCTGGT A^CC[AT]GGT TaqI 121 TCGA T^CGA TfiI 97 GAATC G^A[AT]TC Tsp45I 314 GTGAC ^GT[GC]AC Tth111I 316 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 121 TCGA T^CGA XmnI 435 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvaII 15 GGTCC G^G[AT]CC 411 GGTCC BpmI 136 CTGGAG CTGGAG(16/14) 163 CTGGAG BsmFI 512 GGGAC GGGAC(10/14) 265 GTCCC BstYI 256 AGATCC [GA]^GATC[CT] 279 GGATCC DpnII 257 GATC ^GATC 280 GATC DraII 14 AGGTCCT [GA]G^G[ACGT]CC[CT] 425 AGGCCCT EcoO109I 14 AGGTCCT [GA]G^G[ACGT]CC[CT] 425 AGGCCCT HphI 114 GGTGA GGTGA(8/7) 387 GGTGA MboI 257 GATC ^GATC 280 GATC MboII 33 GAAGA GAAGA(8/7) 473 TCTTC MflI 256 AGATCC [GA]^GATC[CT] 279 GGATCC NdeII 257 GATC ^GATC 280 GATC PstI 346 CTGCAG CTGCA^G 355 CTGCAG SacI 202 GAGCTC GAGCT^C 469 GAGCTC Sau3AI 257 GATC ^GATC 280 GATC SfcI 346 CTGCAG C^T[GA][CT]AG 355 CTGCAG TspRI 170 CAGTG CA[GC]TG[ACGT][ACGT]^ 419 CAGTG XhoII 256 AGATCC [GA]^GATC[CT] 279 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI AlwI AspHI BanII BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI