################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 507 GACGTC GACGT^C AccI 475 GTCTAC GT^[AC][GT]AC AflIII 539 ACACGT A^C[GA][CT]GT Alw44I 664 GTGCAC G^TGCAC AlwI 988 GATCC GGATC(4/5) Aor51HI 1049 AGCGCT AGC^GCT ApaLI 664 GTGCAC G^TGCAC AspI 310 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2 TGGCCA TGG^CCA BanII 648 GGGCTC G[GA]GC[CT]^C BbsI 295 GAAGAC GAAGAC(2/6) BfaI 85 CTAG C^TAG BglI 551 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 351 GCTGAGC GC^T[ACGT]AGC Bpu1102I 351 GCTGAGC GC^T[ACGT]AGC BpuAI 295 GAAGAC GAAGAC(2/6) BseRI 598 CTCCTC GAGGAG(10/8) BsmBI 59 GAGACG CGTCTC(1/5) BspMI 918 GCAGGT ACCTGC(4/8) BsrBI 804 CCGCTC CCGCTC(-3/-3) BsrFI 196 ACCGGC [GA]^CCGG[CT] BstEII 798 GGTCACC G^GT[ACGT]ACC BstPI 798 GGTCACC G^GT[ACGT]ACC BstXI 422 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 351 GCTGAGC GC^T[ACGT]AGC Cfr10I 196 ACCGGC [GA]^CCGG[CT] DrdI 195 GACCGGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1049 AGCGCT AGC^GCT EcoO65I 798 GGTCACC G^GT[ACGT]ACC EcoT14I 422 CCAAGG C^C[AT][AT]GG MaeI 85 CTAG C^TAG MboII 295 GAAGA GAAGA(8/7) MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MseI 328 TTAA T^TAA NruI 1023 TCGCGA TCG^CGA PflFI 310 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 95 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 944 GGGTCCT [GA]G^G[AT]CC[CT] ScaI 152 AGTACT AGT^ACT SmaI 71 CCCGGG CCC^GGG SmlI 913 CTCAAG C^T[CT][GA]AG StuI 233 AGGCCT AGG^CCT StyI 422 CCAAGG C^C[AT][AT]GG TaqI 308 TCGA T^CGA TfiI 752 GATTC G^A[AT]TC Tru9I 328 TTAA T^TAA Tth111I 310 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 308 TCGA T^CGA Van91I 95 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 71 CCCGGG C^CCGGG XmaI 71 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 635 CGCG CG^CG 1024 CGCG AcyI 8 GACGCC G[GA]^CG[CT]C 507 GACGTC AspHI 664 GTGCAC G[AT]GC[AT]^C 768 GTGCTC BsaHI 8 GACGCC G[GA]^CG[CT]C 507 GACGTC BsgI 253 CTGCAC GTGCAG(16/14) 47 GTGCAG BsiHKAI 664 GTGCAC G[AT]GC[AT]^C 768 GTGCTC BsmAI 59 GAGAC GTCTC(1/5) 878 GAGAC BsmFI 275 GGGAC GGGAC(10/14) 505 GGGAC BstAPI 272 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 953 GCATGAGTTGC BstUI 635 CGCG CG^CG 1024 CGCG DpnII 456 GATC ^GATC 988 GATC DsaI 77 CCGTGG C^C[GA][CT]GG 773 CCACGG EcoNI 912 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1037 CCTGCCAGAGG FauI 53 CCCGC CCCGC(4/6) 803 CCCGC HaeII 817 AGCGCC [GA]GCGC^[CT] 1049 AGCGCT HgaI 8 GACGC GACGC(5/10) 61 GACGC Hin1I 8 GACGCC G[GA]^CG[CT]C 507 GACGTC HphI 692 TCACC GGTGA(8/7) 800 TCACC MaeII 508 ACGT A^CGT 541 ACGT MboI 456 GATC ^GATC 988 GATC MvnI 635 CGCG CG^CG 1024 CGCG NdeII 456 GATC ^GATC 988 GATC PstI 546 CTGCAG CTGCA^G 739 CTGCAG Sau3AI 456 GATC ^GATC 988 GATC SfaNI 67 GATGC GCATC(5/9) 500 GCATC TspRI 180 CAGTG CA[GC]TG[ACGT][ACGT]^ 980 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PvuII RsaI Sau96I ScrFI SfcI TseI Tsp45I