(Circular) (Six-base) MAPSORT of: pFN21AE4068.seq Check: 5756 from: 1 to: 5770 pFN21AE4068 5770 bp With 182 enzymes: SgfI * March 28, 2012 13:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2914 2914 Size: 5770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2516 3580 278 Size: 53 83 186 936 980 1064 2468 Fragments arranged by size: 2468 1064 980 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2481 3113 1937 Size: 544 632 4594 Fragments arranged by size: 4594 632 544 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3833 5217 5485 1632 Size: 2201 1384 268 1917 Fragments arranged by size: 2201 1917 1384 268 AfeI AGC'GCT Cuts at: 3056 3056 Size: 5770 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 2544 4669 5646 1203 Size: 1341 2125 977 1327 Fragments arranged by size: 2125 1341 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 2034 2283 2842 3513 5085 2034 Size: 249 559 671 1572 2719 Fragments arranged by size: 2719 1572 671 559 249 ApaLI G'TGCA_C Cuts at: 2669 3827 4983 2669 Size: 1158 1156 3456 Fragments arranged by size: 3456 1158 1156 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2153 2878 3095 1870 Size: 73 133 77 725 217 4545 Fragments arranged by size: 4545 725 217 133 77 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 2423 2486 3091 4546 Size: 325 217 627 404 232 63 605 1455 Cuts at: 4546 618 Size: 1842 Fragments arranged by size: 1842 1455 627 605 404 325 232 217 63 BanII G_rGCy'C Cuts at: 1391 2657 3089 1391 Size: 1266 432 4072 Fragments arranged by size: 4072 1266 432 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2307 928 Size: 971 408 4391 Fragments arranged by size: 4391 971 408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2562 4381 136 Size: 107 122 71 631 605 890 1819 1525 Fragments arranged by size: 1819 1525 890 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 2357 3187 2357 Size: 830 4940 Bme1580I G_kGCm'C Cuts at: 1790 2196 2426 2673 3831 4987 1790 Size: 406 230 247 1158 1156 2573 Fragments arranged by size: 2573 1158 1156 406 247 230 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2116 2178 2326 Size: 416 53 83 399 24 699 62 148 Cuts at: 2326 3501 4459 442 Size: 1175 958 1753 Fragments arranged by size: 1753 1175 958 699 416 399 148 83 62 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2819 2912 2967 4430 1239 Size: 1580 93 55 1463 2579 Fragments arranged by size: 2579 1580 1463 93 55 Bpu10I CC'TnA_GC Cuts at: 2954 2954 Size: 5770 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2903 3895 4760 5058 2903 Size: 992 865 298 3615 Fragments arranged by size: 3615 992 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2014 2513 3577 3959 Size: 53 83 186 936 481 499 1064 382 Cuts at: 3959 4594 275 Size: 635 1451 Fragments arranged by size: 1451 1064 936 635 499 481 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 1178 Size: 30 165 30 726 30 4789 Fragments arranged by size: 4789 726 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 1186 Size: 1408 4362 BseYI C'CCAG_C Cuts at: 2225 2434 2560 2580 2941 2946 3020 4973 5469 Size: 209 126 20 361 5 74 1953 496 Cuts at: 5469 2225 Size: 2526 Fragments arranged by size: 2526 1953 496 361 209 126 74 20 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2073 2243 1163 Size: 573 337 170 4690 Fragments arranged by size: 4690 573 337 170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2673 2777 3831 3916 4987 2673 Size: 104 1054 85 1071 3456 Fragments arranged by size: 3456 1071 1054 104 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 BsmBI CGTCTCn'nnnn_ Cuts at: 2058 2058 Size: 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2426 2657 2673 2777 3089 3831 Size: 399 406 230 231 16 104 312 742 Cuts at: 3831 3916 4987 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 312 231 230 104 85 16 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2914 3125 844 Size: 2070 211 3489 Fragments arranged by size: 3489 2070 211 BsrBI CCG'CTC Cuts at: 1394 1823 2811 3657 1394 Size: 429 988 846 3507 Fragments arranged by size: 3507 988 846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2201 4414 4601 1700 Size: 110 391 2213 187 2869 Fragments arranged by size: 2869 2213 391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 2283 2964 794 Size: 1489 681 3600 Fragments arranged by size: 3600 1489 681 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 2803 1184 Size: 1619 4151 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2434 Size: 5770 BstYI r'GATC_y Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765 Size: 1128 367 385 17 1452 86 12 346 Cuts at: 5765 1972 Size: 1977 Fragments arranged by size: 1977 1452 1128 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2082 2778 513 Size: 552 939 78 696 3505 Fragments arranged by size: 3505 939 696 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 3286 4080 4100 3286 Size: 794 20 4956 Fragments arranged by size: 4956 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2206 4777 809 Size: 507 890 2571 1802 Fragments arranged by size: 2571 1802 890 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2412 2647 Size: 54 1425 50 275 18 177 405 235 Cuts at: 2647 2870 3217 4106 5735 8 Size: 223 347 889 1629 43 Fragments arranged by size: 1629 1425 889 405 347 275 235 223 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3700 1353 Size: 2347 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3001 4357 4731 4877 387 Size: 1064 138 1412 1356 374 146 1280 Fragments arranged by size: 1412 1356 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2819 2912 2967 3833 4430 5217 5485 Size: 393 1187 93 55 866 597 787 268 Cuts at: 5485 1239 Size: 1524 Fragments arranged by size: 1524 1187 866 787 597 393 268 93 55 EcoNI CCTnn'n_nnAGG Cuts at: 2921 3046 2921 Size: 125 5645 EcoO109I rG'GnC_Cy Cuts at: 2031 2584 2950 2031 Size: 553 366 4851 Fragments arranged by size: 4851 553 366 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2398 2647 2398 Size: 249 5521 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 2826 3058 4917 1678 Size: 1148 232 1859 2531 Fragments arranged by size: 2531 1859 1148 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2458 2490 4411 Size: 32 135 32 557 32 297 32 1921 Cuts at: 4411 4443 4485 4517 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1921 557 297 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2172 2496 4884 5068 1305 Size: 867 324 2388 184 2007 Fragments arranged by size: 2388 2007 867 324 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3771 Fragments arranged by size: 3771 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2498 2666 3728 4087 4246 518 Size: 648 1326 6 168 1062 359 159 2042 Fragments arranged by size: 2042 1326 1062 648 359 168 159 6 MspA1I CmG'CkG Cuts at: 1606 2289 2400 2411 2580 2866 2905 3186 3863 Size: 683 111 11 169 286 39 281 677 Cuts at: 3863 5011 5256 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 683 677 286 281 245 169 111 39 11 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NruI TCG'CGA Cuts at: 3030 3030 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 3128 4673 1132 Size: 97 1899 1545 2229 Fragments arranged by size: 2229 1899 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 2106 1108 Size: 998 4772 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2950 2950 Size: 5770 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 2555 2748 3122 830 Size: 1725 193 374 3478 Fragments arranged by size: 3478 1725 374 193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 2400 2580 2866 2400 Size: 180 286 5304 Fragments arranged by size: 5304 286 180 RsrII CG'GwC_CG Cuts at: 3066 3066 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 2159 4018 5730 2159 Size: 1859 1712 2199 Fragments arranged by size: 2199 1859 1712 SfcI C'TryA_G Cuts at: 826 1045 2248 2551 2744 3118 4253 4934 5125 Size: 219 1203 303 193 374 1135 681 191 Cuts at: 5125 826 Size: 1471 Fragments arranged by size: 1471 1203 1135 681 374 303 219 193 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 2078 3097 1872 Size: 206 1019 4545 Fragments arranged by size: 4545 1019 206 SmlI C'TyrA_G Cuts at: 820 1017 1943 2918 3874 4775 5037 820 Size: 197 926 975 956 901 262 1553 Fragments arranged by size: 1553 975 956 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SrfI GCCC'GGGC Cuts at: 2078 2078 Size: 5770 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 2240 1517 Size: 723 5047 StyI C'CwwG_G Cuts at: 513 1065 2004 2427 3209 513 Size: 552 939 423 782 3074 Fragments arranged by size: 3074 939 782 552 423 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3797 3814 3967 4152 4597 325 Size: 1255 2217 17 153 185 445 1498 Fragments arranged by size: 2217 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 2394 4016 Size: 274 80 33 51 1443 180 237 1622 Cuts at: 4016 5728 96 Size: 1712 138 Fragments arranged by size: 1712 1622 1443 274 237 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 2318 1316 Size: 1002 4768 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 2076 3095 1870 Size: 206 1019 4545 Fragments arranged by size: 4545 1019 206 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2514 3578 276 Size: 53 83 186 936 980 1064 2468 Fragments arranged by size: 2468 1064 980 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI