################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MseI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] AfaI 566 GTAC GT^AC ApoI 31 GAATTT [GA]^AATT[CT] AviII 915 TGCGCA TGC^GCA BalI 371 TGGCCA TGG^CCA BbvI 339 GCTGC GCAGC(8/12) BfaI 697 CTAG C^TAG BglII 299 AGATCT A^GATCT BpmI 583 CTGGAG CTGGAG(16/14) BsmAI 897 GAGAC GTCTC(1/5) BsmFI 45 GGGAC GGGAC(10/14) BspHI 404 TCATGA T^CATGA BsrDI 889 GCAATG GCAATG(2/0) BstXI 312 CCATGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 299 AGATCT [GA]^GATC[CT] Cac8I 109 GCGAGC GC[ACGT]^[ACGT]GC CfoI 916 GCGC GCG^C DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 16 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 899 GACTGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 371 TGGCCA [CT]^GGCC[GA] EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT22I 824 ATGCAT ATGCA^T Fnu4HI 339 GCTGC GC^[ACGT]GC FspI 915 TGCGCA TGC^GCA HhaI 916 GCGC GCG^C HinP1I 916 GCGC G^CGC HphI 50 TCACC GGTGA(8/7) ItaI 339 GCTGC GC^[ACGT]GC MaeI 697 CTAG C^TAG MflI 299 AGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA NdeI 375 CATATG CA^TATG NsiI 824 ATGCAT ATGCA^T RcaI 404 TCATGA T^CATGA RsaI 566 GTAC GT^AC TseI 339 GCTGC G^C[AT]GC XcmI 788 CCACATCACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 299 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AluI 71 AGCT AG^CT 723 AGCT AvaII 471 GGTCC G^G[AT]CC 508 GGTCC BanII 408 GAGCCC G[GA]GC[CT]^C 624 GGGCTC BsaBI 154 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 802 GATAAATATC BseRI 550 CTCCTC GAGGAG(10/8) 894 GAGGAG BsgI 163 CTGCAC GTGCAG(16/14) 903 GTGCAG BsrI 582 ACTGG ACTGG(1/-1) 850 CCAGT BssKI 186 CCTGG ^CC[ACGT]GG 798 CCTGG BstF5I 153 GGATG GGATG(2/0) 286 GGATG BstNI 186 CCTGG CC^[AT]GG 798 CCTGG DpnII 300 GATC ^GATC 538 GATC DsaI 149 CCATGG C^C[GA][CT]GG 350 CCATGG EcoRII 186 CCTGG ^CC[AT]GG 798 CCTGG FokI 153 GGATG GGATG(9/13) 286 GGATG MamI 154 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 802 GATAAATATC MboI 300 GATC ^GATC 538 GATC MvaI 186 CCTGG CC^[AT]GG 798 CCTGG NcoI 149 CCATGG C^CATGG 350 CCATGG NdeII 300 GATC ^GATC 538 GATC NlaIV 548 GGCTCC GG[ACGT]^[ACGT]CC 625 GGCTCC PflMI 462 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 576 CCAAGAACTGG PleI 47 GACTC GAGTC(4/5) 611 GACTC Sau3AI 300 GATC ^GATC 538 GATC ScrFI 186 CCTGG CC^[ACGT]GG 798 CCTGG SfaNI 154 GATGC GCATC(5/9) 823 GATGC Tsp45I 595 GTCAC ^GT[GC]AC 730 GTCAC Van91I 462 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 576 CCAAGAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### BmyI BsaJI BsiYI BslI Bsp1286I Cfr13I DdeI EcoT14I HaeIII HinfI MaeIII MboII MnlI MslI NlaIII Sau96I StyI TfiI Tsp509I TspRI