################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspA1I MspI MunI MwoI NaeI NarI NciI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 490 GTAGAC GT^[AC][GT]AC AccII 59 CGCG CG^CG AciI 60 GCGG CCGC(-3/-1) AcsI 805 AAATTC [GA]^AATT[CT] AfaI 177 GTAC GT^AC AlwI 66 GATCC GGATC(4/5) ApoI 805 AAATTC [GA]^AATT[CT] AseI 759 ATTAAT AT^TAAT AsnI 759 ATTAAT AT^TAAT BbsI 896 GAAGAC GAAGAC(2/6) BbvI 339 GCTGC GCAGC(8/12) BglII 299 AGATCT A^GATCT BpuAI 896 GAAGAC GAAGAC(2/6) BsiYI 363 CCGATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 363 CCGATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspHI 404 TCATGA T^CATGA BspMI 494 ACCTGC ACCTGC(4/8) BsrDI 889 GCAATG GCAATG(2/0) BstF5I 3 GGATG GGATG(2/0) BstUI 59 CGCG CG^CG BstXI 312 CCATGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 350 CCATGG C^C[GA][CT]GG EarI 79 CTCTTC CTCTTC(1/4) EcoT14I 350 CCATGG C^C[AT][AT]GG Fnu4HI 339 GCTGC GC^[ACGT]GC FokI 3 GGATG GGATG(9/13) HphI 666 GGTGA GGTGA(8/7) ItaI 339 GCTGC GC^[ACGT]GC Ksp632I 79 CTCTTC CTCTTC(1/4) MvnI 59 CGCG CG^CG NcoI 350 CCATGG C^CATGG NdeI 375 CATATG CA^TATG PshBI 759 ATTAAT AT^TAAT RcaI 404 TCATGA T^CATGA RsaI 177 GTAC GT^AC StyI 350 CCATGG C^C[AT][AT]GG TaqI 546 TCGA T^CGA TseI 339 GCTGC G^C[AT]GC TthHB8I 546 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### BalI 188 TGGCCA TGG^CCA 371 TGGCCA BsaI 571 GAGACC GGTCTC(1/5) 24 GGTCTC BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG 350 CCATGG BsmAI 571 GAGAC GTCTC(1/5) 25 GTCTC BsmFI 45 GGGAC GGGAC(10/14) 168 GTCCC BsrI 558 ACTGG ACTGG(1/-1) 850 CCAGT BssKI 42 CCTGG ^CC[ACGT]GG 443 CCTGG BstNI 42 CCTGG CC^[AT]GG 443 CCTGG BstYI 65 AGATCC [GA]^GATC[CT] 299 AGATCT Cac8I 186 GCTGGC GC[ACGT]^[ACGT]GC 562 GCTTGC Cfr13I 508 GGCCC G^G[ACGT]CC 754 GGGCC DraIII 16 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 463 CACTCAGTG EaeI 188 TGGCCA [CT]^GGCC[GA] 371 TGGCCA EcoRII 42 CCTGG ^CC[AT]GG 443 CCTGG MflI 65 AGATCC [GA]^GATC[CT] 299 AGATCT MluNI 188 TGGCCA TGG^CCA 371 TGGCCA MscI 188 TGGCCA TGG^CCA 371 TGGCCA MseI 634 TTAA T^TAA 760 TTAA MvaI 42 CCTGG CC^[AT]GG 443 CCTGG PleI 47 GACTC GAGTC(4/5) 55 GACTC Sau96I 508 GGCCC G^G[ACGT]CC 754 GGGCC ScrFI 42 CCTGG CC^[ACGT]GG 443 CCTGG SfaNI 823 GATGC GCATC(5/9) 902 GATGC Tru9I 634 TTAA T^TAA 760 TTAA Tsp45I 145 GTGAC ^GT[GC]AC 667 GTGAC TspRI 95 CAGTG CA[GC]TG[ACGT][ACGT]^ 467 CAGTG XcmI 11 CCAATCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 200 CCATCATCAATTTGG XhoII 65 AGATCC [GA]^GATC[CT] 299 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI BseRI DdeI DpnII HaeIII HinfI MaeIII MboI MboII MnlI MslI NdeII NlaIII Sau3AI TfiI Tsp509I