Restriction map for
pFN21AE6380
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeIII MamI MfeI
MluI MluNI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 30 GGTACC G^GTACC
AccII 301 CGCG CG^CG
AccIII 644 TCCGGA T^CCGGA
AcsI 354 GAATTT [GA]^AATT[CT]
AhdI 385 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 398 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 354 GAATTT [GA]^AATT[CT]
Asp718I 30 GGTACC G^GTACC
AspEI 385 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 30 GGTACC G^G[CT][GA]CC
BlpI 904 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 904 GCTGAGC GC^T[ACGT]AGC
BsaI 169 GAGACC GGTCTC(1/5)
BsaWI 644 TCCGGA [AT]^CCGG[AT]
BseAI 644 TCCGGA T^CCGGA
BspDI 838 ATCGAT AT^CGAT
BspEI 644 TCCGGA T^CCGGA
BstUI 301 CGCG CG^CG
CelII 904 GCTGAGC GC^T[ACGT]AGC
ClaI 838 ATCGAT AT^CGAT
Eam1105I 385 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 122 CCCGC CCCGC(4/6)
HinfI 685 GATTC G^A[ACGT]TC
KpnI 30 GGTACC GGTAC^C
MroI 644 TCCGGA T^CCGGA
MslI 805 CACGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 301 CGCG CG^CG
NspI 674 ACATGC [GA]CATG^[CT]
PvuII 115 CAGCTG CAG^CTG
SfaNI 596 GCATC GCATC(5/9)
SmaI 173 CCCGGG CCC^GGG
SmlI 787 CTTGAG C^T[CT][GA]AG
TaqI 839 TCGA T^CGA
TfiI 685 GATTC G^A[AT]TC
Tsp509I 355 AATT ^AATT
TspRI 185 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 839 TCGA T^CGA
XmaCI 173 CCCGGG C^CCGGG
XmaI 173 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 49 GAGCTC G[AT]GC[AT]^C
376 GAGCTC
AvaI 173 CCCGGG C^[CT]CG[GA]G
225 CTCGGG
AvaII 84 GGACC G^G[AT]CC
91 GGACC
BanII 49 GAGCTC G[GA]GC[CT]^C
376 GAGCTC
BfaI 446 CTAG C^TAG
638 CTAG
BglII 524 AGATCT A^GATCT
654 AGATCT
BsiHKAI 49 GAGCTC G[AT]GC[AT]^C
376 GAGCTC
BsiYI 94 CCTGTCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
174 CCGGGAGGAGG
BslI 94 CCTGTCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
174 CCGGGAGGAGG
BsmAI 169 GAGAC GTCTC(1/5)
641 GTCTC
BsmFI 452 GGGAC GGGAC(10/14)
393 GTCCC
BsoBI 173 CCCGGG C^[CT]CG[GA]G
225 CTCGGG
BsrI 63 ACTGG ACTGG(1/-1)
494 ACTGG
BstYI 524 AGATCT [GA]^GATC[CT]
654 AGATCT
EaeI 245 TGGCCG [CT]^GGCC[GA]
462 TGGCCG
HphI 512 TCACC GGTGA(8/7)
740 TCACC
MaeI 446 CTAG C^TAG
638 CTAG
MaeII 19 ACGT A^CGT
317 ACGT
MflI 524 AGATCT [GA]^GATC[CT]
654 AGATCT
NcoI 87 CCATGG C^CATGG
730 CCATGG
NlaIV 30 GGTACC GG[ACGT]^[ACGT]CC
919 GGCCCC
PstI 196 CTGCAG CTGCA^G
364 CTGCAG
SacI 49 GAGCTC GAGCT^C
376 GAGCTC
SapI 774 GAAGAGC GCTCTTC(1/4)
477 GCTCTTC
SfcI 196 CTGCAG C^T[GA][CT]AG
364 CTGCAG
XhoII 524 AGATCT [GA]^GATC[CT]
654 AGATCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI Ksp632I MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI StyI TseI