(Circular) (Six-base) MAPSORT of: pFN21AE2450.seq Check: 8501 from: 1 to: 5668 pFN21AE2450 5668 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2329 2989 2329 Size: 660 5008 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3731 5115 5383 1632 Size: 2099 1384 268 1917 Fragments arranged by size: 2099 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 3411 4983 3411 Size: 1572 4096 ApaLI G'TGCA_C Cuts at: 2645 3725 4881 2645 Size: 1080 1156 3432 Fragments arranged by size: 3432 1156 1080 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2504 2741 2993 1870 Size: 73 561 237 252 4545 Fragments arranged by size: 4545 561 252 237 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 2584 2989 4444 618 Size: 325 217 627 542 255 405 1455 1842 Fragments arranged by size: 1842 1455 627 542 405 325 255 217 BanII G_rGCy'C Cuts at: 1391 2109 2987 1391 Size: 718 878 4072 Fragments arranged by size: 4072 878 718 BbeI G_GCGC'C Cuts at: 2588 2588 Size: 5668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2275 2884 928 Size: 971 376 609 3712 Fragments arranged by size: 3712 971 609 376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3859 3893 1903 Size: 34 50 34 1838 34 3678 Fragments arranged by size: 3678 1838 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 2364 1548 Size: 816 4852 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2640 4279 136 Size: 107 122 71 631 605 968 1639 1525 Fragments arranged by size: 1639 1525 968 631 605 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 2649 3729 4885 1790 Size: 859 1080 1156 2573 Fragments arranged by size: 2573 1156 1080 859 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2338 3399 4357 Size: 416 53 83 399 24 921 1061 958 Cuts at: 4357 442 Size: 1753 Fragments arranged by size: 1753 1061 958 921 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2792 4328 1239 Size: 1553 1536 2579 Fragments arranged by size: 2579 1553 1536 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2585 3475 3857 4492 Size: 53 83 186 936 1052 890 382 635 Cuts at: 4492 275 Size: 1451 Fragments arranged by size: 1451 1052 936 890 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2936 2966 1178 Size: 30 165 30 1533 30 3880 Fragments arranged by size: 3880 1533 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2089 2789 1186 Size: 903 700 4065 Fragments arranged by size: 4065 903 700 BseYI C'CCAG_C Cuts at: 4871 5367 4871 Size: 496 5172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2708 2890 1163 Size: 573 972 182 3941 Fragments arranged by size: 3941 972 573 182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2649 2718 3729 3814 4885 2190 Size: 459 69 1011 85 1071 2973 Fragments arranged by size: 2973 1071 1011 459 85 69 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2190 2649 2718 2987 3729 3814 Size: 399 319 81 459 69 269 742 85 Cuts at: 3814 4885 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 459 399 319 269 85 81 69 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2619 3023 844 Size: 1334 441 404 3489 Fragments arranged by size: 3489 1334 441 404 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 4163 4337 65 Size: 1183 1539 1376 174 1396 Fragments arranged by size: 1539 1396 1376 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 2616 96 Size: 2520 3148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 2294 3728 4740 2294 Size: 1434 1012 3222 Fragments arranged by size: 3222 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2694 2722 794 Size: 1900 28 3740 Fragments arranged by size: 3740 1900 28 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstXI CCAn_nnnn'nTGG Cuts at: 2770 2941 2770 Size: 171 5497 BstYI r'GATC_y Cuts at: 1972 2766 2998 3365 3750 3767 5219 5305 5317 Size: 794 232 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 794 385 367 346 232 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2580 2629 513 Size: 552 1515 49 3552 Fragments arranged by size: 3552 1515 552 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 3184 3978 3998 3184 Size: 794 20 4854 Fragments arranged by size: 4854 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2559 2683 2760 Size: 54 1425 50 275 18 729 124 77 Cuts at: 2760 3115 4004 5633 8 Size: 355 889 1629 43 Fragments arranged by size: 1629 1425 889 729 355 275 124 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2492 3598 1353 Size: 1139 1106 3423 Fragments arranged by size: 3423 1139 1106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2185 4255 4629 4775 387 Size: 1064 138 596 2070 374 146 1280 Fragments arranged by size: 2070 1280 1064 596 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2792 3731 4328 5115 5383 1239 Size: 393 1160 939 597 787 268 1524 Fragments arranged by size: 1524 1160 939 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2774 2774 Size: 5668 EcoO109I rG'GnC_Cy Cuts at: 2050 2749 2050 Size: 699 4969 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 EcoRV GAT'ATC Cuts at: 2020 2020 Size: 5668 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2551 2580 2588 4815 1678 Size: 873 29 8 2227 2531 Fragments arranged by size: 2531 2227 873 29 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2914 2936 2946 2968 4309 Size: 32 135 32 1342 22 10 22 1341 Cuts at: 4309 4341 4383 4415 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1342 1341 135 42 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KasI G'GCGC_C Cuts at: 2584 2584 Size: 5668 KpnI G_GTAC'C Cuts at: 2333 2993 2333 Size: 660 5008 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2561 2762 10 Size: 54 1425 343 729 201 2916 Fragments arranged by size: 2916 1425 729 343 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2921 3626 3985 4144 518 Size: 648 1755 705 359 159 2042 Fragments arranged by size: 2042 1755 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2545 2631 2805 3084 3761 4909 5154 1606 Size: 939 86 174 279 677 1148 245 2120 Fragments arranged by size: 2120 1148 939 677 279 245 174 86 NarI GG'CG_CC Cuts at: 2585 2585 Size: 5668 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5116 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 3026 4571 1132 Size: 97 1797 1545 2229 Fragments arranged by size: 2229 1797 1545 97 PasI CC'CwG_GG Cuts at: 1348 2251 1348 Size: 903 4765 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5668 PfoI T'CCnGG_A Cuts at: 2769 2769 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2828 2860 3737 3769 5250 5282 2828 Size: 32 877 32 1481 32 3214 Fragments arranged by size: 3214 1481 877 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2050 2749 2050 Size: 699 4969 PshAI GACnn'nnGTC Cuts at: 1613 2456 1613 Size: 843 4825 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2600 2632 2600 Size: 32 5636 PstI C_TGCA'G Cuts at: 830 2230 2409 2555 2834 3020 830 Size: 1400 179 146 279 186 3478 Fragments arranged by size: 3478 1400 279 186 179 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SacII CC_GC'GG Cuts at: 2632 2632 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SanDI GG'GwC_CC Cuts at: 2749 2749 Size: 5668 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 2348 3916 5628 2348 Size: 1568 1712 2388 Fragments arranged by size: 2388 1712 1568 SfcI C'TryA_G Cuts at: 826 1045 2226 2405 2551 2830 3016 4151 4832 Size: 219 1181 179 146 279 186 1135 681 Cuts at: 4832 5023 826 Size: 191 1471 Fragments arranged by size: 1471 1181 1135 681 279 219 191 186 179 146 SfoI GGC'GCC Cuts at: 2586 2586 Size: 5668 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2506 2995 1872 Size: 634 489 4545 Fragments arranged by size: 4545 634 489 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 3107 513 Size: 552 2042 3074 Fragments arranged by size: 3074 2042 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2878 3695 3712 3865 4050 4495 325 Size: 1255 1298 817 17 153 185 445 1498 Fragments arranged by size: 1498 1298 1255 817 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 2616 3914 Size: 274 80 33 51 1443 369 270 1298 Cuts at: 3914 5626 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1298 369 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2855 2887 5628 5660 2855 Size: 32 2741 32 2863 Fragments arranged by size: 2863 2741 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2368 2368 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2504 2993 1870 Size: 634 489 4545 Fragments arranged by size: 4545 634 489 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI