################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 125 CGCG CG^CG AfaI 285 GTAC GT^AC Alw44I 181 GTGCAC G^TGCAC AlwNI 303 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 181 GTGCAC G^TGCAC AspHI 181 GTGCAC G[AT]GC[AT]^C AvaII 354 GGTCC G^G[AT]CC BalI 327 TGGCCA TGG^CCA BamHI 50 GGATCC G^GATCC BanI 30 GGTGCC G^G[CT][GA]CC BfaI 25 CTAG C^TAG BsiHKAI 181 GTGCAC G[AT]GC[AT]^C BsrI 302 CCAGT ACTGG(1/-1) BstUI 125 CGCG CG^CG BstXI 54 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 50 GGATCC [GA]^GATC[CT] Cac8I 252 GCCCGC GC[ACGT]^[ACGT]GC DpnII 51 GATC ^GATC DraII 142 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 324 CCATGG C^C[GA][CT]GG EcoNI 122 CCTCGCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 142 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 324 CCATGG C^C[AT][AT]GG FauI 253 CCCGC CCCGC(4/6) HgaI 21 GCGTC GACGC(5/10) HindIII 11 AAGCTT A^AGCTT HphI 96 GGTGA GGTGA(8/7) MaeI 25 CTAG C^TAG MaeIII 97 GTGAC ^GT[ACGT]AC MboI 51 GATC ^GATC MboII 73 GAAGA GAAGA(8/7) MflI 50 GGATCC [GA]^GATC[CT] MluNI 327 TGGCCA TGG^CCA MscI 327 TGGCCA TGG^CCA MslI 174 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 125 CGCG CG^CG NcoI 324 CCATGG C^CATGG NdeII 51 GATC ^GATC NlaIII 325 CATG CATG^ PflMI 302 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 337 GACTC GAGTC(4/5) PshAI 99 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 285 GTAC GT^AC Sau3AI 51 GATC ^GATC StuI 58 AGGCCT AGG^CCT StyI 324 CCATGG C^C[AT][AT]GG TfiI 298 GATTC G^A[AT]TC Tsp45I 97 GTGAC ^GT[GC]AC Van91I 302 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 50 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 51 GATCC GGATC(4/5) 50 GGATC AvaI 209 CCCGGG C^[CT]CG[GA]G 274 CCCGGG BmyI 31 GTGCCC G[AGT]GC[ACT]^C 181 GTGCAC BseRI 222 CTCCTC GAGGAG(10/8) 79 GAGGAG BsoBI 209 CCCGGG C^[CT]CG[GA]G 274 CCCGGG Bsp1286I 31 GTGCCC G[AGT]GC[ACT]^C 181 GTGCAC HinfI 298 GATTC G^A[ACGT]TC 337 GACTC MwoI 4 GCCTCCGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 183 GCACTTGTGGC NlaIV 30 GGTGCC GG[ACGT]^[ACGT]CC 50 GGATCC PvuII 247 CAGCTG CAG^CTG 349 CAGCTG SmaI 209 CCCGGG CCC^GGG 274 CCCGGG TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^ 200 CAGTG XmaCI 209 CCCGGG C^CCGGG 274 CCCGGG XmaI 209 CCCGGG C^CCGGG 274 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI BbvI BcnI BsaJI BsiYI BslI BssKI BstNI Cfr13I EaeI EcoRII Fnu4HI HaeIII HapII HpaII ItaI MnlI MspA1I MspI MvaI NciI Sau96I ScrFI SfaNI TseI