(Circular) (Six-base) MAPSORT of: pFN21AE2268.seq Check: 7661 from: 1 to: 5089 pFN21AE2268 5089 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2899 278 Size: 53 83 186 936 1363 2468 Fragments arranged by size: 2468 1363 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2432 1937 Size: 495 4594 Acc65I G'GTAC_C Cuts at: 2410 2410 Size: 5089 AclI AA'CG_TT Cuts at: 1563 3216 3589 1563 Size: 1653 373 3063 Fragments arranged by size: 3063 1653 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3152 4536 4804 1632 Size: 1520 1384 268 1917 Fragments arranged by size: 1917 1520 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4892 AflIII A'CryG_T Cuts at: 1203 3988 4965 1203 Size: 2785 977 1327 Fragments arranged by size: 2785 1327 977 AgeI A'CCGG_T Cuts at: 3920 3920 Size: 5089 AhdI GACnn_n'nnGTC Cuts at: 3818 3818 Size: 5089 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5089 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2201 1184 Size: 1017 4072 AlwNI CAG_nnn'CTG Cuts at: 2313 2832 4404 2313 Size: 519 1572 2998 Fragments arranged by size: 2998 1572 519 ApaLI G'TGCA_C Cuts at: 2186 3146 4302 2186 Size: 960 1156 2973 Fragments arranged by size: 2973 1156 960 ApoI r'AATT_y Cuts at: 1493 2398 2624 2776 4885 1493 Size: 905 226 152 2109 1697 Fragments arranged by size: 2109 1697 905 226 152 AseI AT'TA_AT Cuts at: 160 1032 3643 160 Size: 872 2611 1606 Fragments arranged by size: 2611 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5089 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2279 2414 1870 Size: 73 271 65 135 4545 Fragments arranged by size: 4545 271 135 73 65 BamHI G'GATC_C Cuts at: 2055 2419 2786 2055 Size: 364 367 4358 Fragments arranged by size: 4358 367 364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2410 3865 618 Size: 325 217 627 248 375 1455 1842 Fragments arranged by size: 1842 1455 627 375 325 248 217 BanII G_rGCy'C Cuts at: 1391 2408 1391 Size: 1017 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3280 3314 1903 Size: 34 1343 34 3678 Fragments arranged by size: 3678 1343 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2981 4191 2981 Size: 1210 3879 BclI T'GATC_A Cuts at: 1548 1548 Size: 5089 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3700 136 Size: 107 122 71 631 605 2028 1525 Fragments arranged by size: 2028 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5084 5084 Size: 5089 BlpI GC'TnA_GC Cuts at: 2506 2506 Size: 5089 Bme1580I G_kGCm'C Cuts at: 1790 2040 2190 3150 4306 1790 Size: 250 150 960 1156 2573 Fragments arranged by size: 2573 1156 960 250 150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2820 3778 442 Size: 416 53 83 399 24 1403 958 1753 Fragments arranged by size: 1753 1403 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5089 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 5089 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3749 1239 Size: 2510 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3214 4079 4377 3214 Size: 865 298 3926 Fragments arranged by size: 3926 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3752 882 Size: 617 2253 2219 Fragments arranged by size: 2253 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2880 493 Size: 711 1676 2702 Fragments arranged by size: 2702 1676 711 BsaBI GATnn'nnATC Cuts at: 5083 5083 Size: 5089 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2896 3278 3913 275 Size: 53 83 186 936 1363 382 635 1451 Fragments arranged by size: 1451 1363 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3522 3920 4194 4341 4752 3522 Size: 398 274 147 411 3859 Fragments arranged by size: 3859 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4864 Fragments arranged by size: 4864 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2218 1186 Size: 913 119 4057 Fragments arranged by size: 4057 913 119 BseYI C'CCAG_C Cuts at: 2174 2250 4292 4788 2174 Size: 76 2042 496 2475 Fragments arranged by size: 2475 2042 496 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2539 3300 4328 1540 Size: 275 186 538 761 1028 2301 Fragments arranged by size: 2301 1028 761 538 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 3150 3235 4306 2190 Size: 960 85 1071 2973 Fragments arranged by size: 2973 1071 960 85 BsmI GAATG_Cn' Cuts at: 2600 2693 2600 Size: 93 4996 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2190 2408 3150 3235 4306 1391 Size: 399 250 150 218 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 250 218 150 85 BspEI T'CCGG_A Cuts at: 4752 4752 Size: 5089 BspHI T'CATG_A Cuts at: 2978 4708 2978 Size: 1730 3359 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2444 844 Size: 1600 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2976 1394 Size: 429 1153 3507 Fragments arranged by size: 3507 1153 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3584 3758 65 Size: 1183 2336 174 1396 Fragments arranged by size: 2336 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3733 3920 1700 Size: 110 1923 187 2869 Fragments arranged by size: 2869 1923 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5089 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5089 BssSI C'ACGA_G Cuts at: 3149 4161 3149 Size: 1012 4077 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5089 BstBI TT'CG_AA Cuts at: 3893 3893 Size: 5089 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5089 BstXI CCAn_nnnn'nTGG Cuts at: 2066 2066 Size: 5089 BstYI r'GATC_y Cuts at: 1972 2055 2419 2786 3171 3188 4640 4726 4738 Size: 83 364 367 385 17 1452 86 12 Cuts at: 4738 5084 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 364 346 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2329 513 Size: 552 939 325 3273 Fragments arranged by size: 3273 939 552 325 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4350 Fragments arranged by size: 4350 683 56 BtsI GCAGTG_nn' Cuts at: 2211 2605 3399 3419 2211 Size: 394 794 20 3881 Fragments arranged by size: 3881 794 394 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5089 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5054 DraI TTT'AAA Cuts at: 2393 2393 Size: 5089 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4096 809 Size: 507 2780 1802 Fragments arranged by size: 2780 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2233 2332 Size: 54 1425 50 275 18 214 189 99 Cuts at: 2332 2536 3425 5054 8 Size: 204 889 1629 43 Fragments arranged by size: 1629 1425 889 275 214 204 189 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2536 1537 Size: 275 724 4090 Fragments arranged by size: 4090 724 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3019 1353 Size: 1666 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3676 4050 4196 387 Size: 1064 138 2087 374 146 1280 Fragments arranged by size: 2087 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3152 3749 4536 4804 1239 Size: 393 1520 597 787 268 1524 Fragments arranged by size: 1524 1520 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2131 2131 Size: 5089 EcoO109I rG'GnC_Cy Cuts at: 2148 2148 Size: 5089 EcoRI G'AATT_C Cuts at: 2398 2776 2398 Size: 378 4711 FspI TGC'GCA Cuts at: 3595 3595 Size: 5089 HaeII r_GCGC'y Cuts at: 1678 4236 1678 Size: 2558 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3730 3762 3804 3836 1373 Size: 32 135 32 2158 32 42 32 2626 Fragments arranged by size: 2626 2158 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2433 2679 669 Size: 988 281 495 246 3079 Fragments arranged by size: 3079 988 495 281 246 HindIII A'AGCT_T Cuts at: 748 2016 748 Size: 1268 3821 HpaI GTT'AAC Cuts at: 2679 2679 Size: 5089 KpnI G_GTAC'C Cuts at: 2414 2414 Size: 5089 MfeI C'AATT_G Cuts at: 2688 2688 Size: 5089 MluI A'CGCG_T Cuts at: 4965 4965 Size: 5089 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4203 4387 1305 Size: 2898 184 2007 Fragments arranged by size: 2898 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2334 10 Size: 54 1425 343 502 2765 Fragments arranged by size: 2765 1425 502 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 3047 3406 3565 518 Size: 648 1017 864 359 159 2042 Fragments arranged by size: 2042 1017 864 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2254 2300 2356 2505 3182 4330 4575 1606 Size: 648 46 56 149 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 648 245 149 56 46 NcoI C'CATG_G Cuts at: 513 1065 2004 2329 513 Size: 552 939 325 3273 Fragments arranged by size: 3273 939 552 325 NdeI CA'TA_TG Cuts at: 387 387 Size: 5089 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5089 NotI GC'GGCC_GC Cuts at: 2536 2536 Size: 5089 NspI r_CATG'y Cuts at: 1132 1229 2447 3992 1132 Size: 97 1218 1545 2229 Fragments arranged by size: 2229 1545 1218 97 PasI CC'CwG_GG Cuts at: 1348 2155 1348 Size: 807 4282 PciI A'CATG_T Cuts at: 3988 3988 Size: 5089 PflMI CCAn_nnn'nTGG Cuts at: 1108 2313 1108 Size: 1205 3884 PmeI GTTT'AAAC Cuts at: 2393 2393 Size: 5089 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3158 3190 4671 4703 3158 Size: 32 1481 32 3544 Fragments arranged by size: 3544 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2108 1613 Size: 495 4594 PsiI TTA'TAA Cuts at: 2659 2659 Size: 5089 PstI C_TGCA'G Cuts at: 830 2441 830 Size: 1611 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5089 PvuII CAG'CTG Cuts at: 2254 2356 2254 Size: 102 4987 SalI G'TCGA_C Cuts at: 1936 2431 1936 Size: 495 4594 SbfI CC_TGCA'GG Cuts at: 2441 2441 Size: 5089 ScaI AGT'ACT Cuts at: 3337 5049 3337 Size: 1712 3377 SfcI C'TryA_G Cuts at: 826 1045 2437 3572 4253 4444 826 Size: 219 1392 1135 681 191 1471 Fragments arranged by size: 1471 1392 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5089 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5089 SmaI CCC'GGG Cuts at: 1872 2216 2281 2416 1872 Size: 344 65 135 4545 Fragments arranged by size: 4545 344 135 65 SmlI C'TyrA_G Cuts at: 820 1017 1943 3193 4094 4356 820 Size: 197 926 1250 901 262 1553 Fragments arranged by size: 1553 1250 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2880 493 Size: 2387 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5089 SphI G_CATG'C Cuts at: 1132 2447 1132 Size: 1315 3774 SspI AAT'ATT Cuts at: 5 52 3013 5 Size: 47 2961 2081 Fragments arranged by size: 2961 2081 47 StuI AGG'CCT Cuts at: 1517 2065 1517 Size: 548 4541 StyI C'CwwG_G Cuts at: 513 1065 2004 2329 2528 513 Size: 552 939 325 199 3074 Fragments arranged by size: 3074 939 552 325 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2089 3116 3133 3286 3471 3916 325 Size: 1255 509 1027 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1027 509 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3335 5047 96 Size: 274 80 33 51 1443 1358 1712 138 Fragments arranged by size: 1712 1443 1358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5049 5081 5049 Size: 32 5057 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5089 XbaI T'CTAG_A Cuts at: 2425 2425 Size: 5089 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5089 XmaI C'CCGG_G Cuts at: 1870 2214 2279 2414 1870 Size: 344 65 135 4545 Fragments arranged by size: 4545 344 135 65 XmnI GAAnn'nnTTC Cuts at: 3218 3218 Size: 5089 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2897 276 Size: 53 83 186 936 1363 2468 Fragments arranged by size: 2468 1363 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI